3-1
Techniques of Sterilization
Operating Instructions
129373-496
The information in this section is intended as a general guide to steam
sterilization techniques.
STERIS also recommends reference to the standards of Association for the
Advancement of Medical Instrumentation (AAMI).
Prior to sterilization, all materials and articles must be thoroughly cleaned.
After sterilization, most goods should be stored for no longer than 30 days,
depending on wrapping materials.
For sterilization of articles or materials not covered in this section, contact the
manufacturer of the article for recommended procedure. Cycle times and
temperatures not covered in this manual should always be validated for
efficacy before processing loads.*
* For in-depth training, STERIS offers a wide range of education/training programs
designed to meet the educational needs of scientific industries. Contact STERIS for
details.
3.1 General
TECHNIQUES OF STERILIZATION
3
3.2.1 Prevacuum Cycle
Table 3-1. Prevacuum Cycle Parameters
Pressure
Minimum Recommended
Temperature
Point
Sterilize Time*
psig (psia)
Minutes at Temperature
132°C (270°F)
26-28 (40-42)
4
* Minimum sterilize times are based on obtaining a 10
-6
Sterility Assurance Level
(SAL) with standard test loads. Your specific loads may require different sterilize
times to achieve this level of sterility, or you may require a different SAL.
3.2 Recommended
Sterilization
Variables
Prevacuum cycle is recommended to process heat- and moisture-stabile
goods, except liquids, which are capable of being sterilized with steam. This
cycle can also be used to decontaminate wastes, including wastes containing
liquids, provided the materials are properly contained.
Refer to
Table 3-1
for recommended Prevacuum cycle parameters.
