AST 550
（
Version
：
AMOSAST550OP20130402
）
Fully-automatic Cryostat Microtome
Operation Manual
www.amos-scientific.com
AMOS SCIENTIFIC PTY.LTD. ABN37 159 778 140
Page 1: ...AST 550 Version AMOSAST550OP20130402 Fully automatic Cryostat Microtome Operation Manual www amos scientific com AMOS SCIENTIFIC PTY LTD ABN37 159 778 140 ...
Page 2: ...Presented in this Operation manual are the structure functions and using notice Reading carefully prior to operating the instrument ...
Page 3: ...rably it is necessary to read the operation manual carefully Our company are not only responsible for the repair but also train distributors ability of repairing To repair the product smoothly in time please contact your local distributor Notice With the development of technology and constantly updating of products this manual will make the corresponding changes Please forgive us not to notify the...
Page 4: ...uirement of Installation 7 3 2 Standard Delivery 7 3 3 Installation 8 3 4 Electrical Connection 10 4 Operation 11 4 1 Operate Panel Function Control 11 4 2 Installation of Blade Holder 16 4 3Clamping The Specimen Inserting The Blade 18 4 4 Temperature Setting 19 4 5 Time Setting 20 4 6 Section Setting 22 4 7 Retraction Setting 24 4 8 Section Reference 24 5 Clearance Maintenance 25 5 1 cleaning 25 ...
Page 5: ...ilt angle should not exceed 45 Prior to removing the microtome remove the blade holder The input voltage has been preset at the factory please check this setting complies with your local power requirement before connecting to the main power Please use the power cord provided If need to replace it please use the power cord with earth wire Do not operate this instrument in the room with the danger o...
Page 6: ...Turn the instrument off and unplug the power cord before changing the fuse Please use the fuse according to the requirement and specification in operation manual 1 3 Safety Device Handwheel locking mechanism As showed in Fig it is the lock lever Clockwise push the lever 1 until it lock the handwheel at the upright position To unlock the handwheel rotate counterclockwise the lever 1 As showed in Fi...
Page 7: ...gency stop The emergency stop function is activated with the red switch 2 pressed while the main power off and all action stops To deactivate this function turn the switch in the direction of the arrow Correct use in order Activate emergency switch turn off power switch deactivate turn on power switch ...
Page 8: ...rs 2 1Overview Instrument Components Display Control Panel Control Panel Handwheel Blade Holder Specimen Clamp Lock Lever Waste Container Tool Shelf Heating Glass Stationary Heat Extractor Quick Freeze Shelf Peltier on freeze shelf freezing Area Control Panel E stop switch ...
Page 9: ...ate Adopt UV and O3 to sterilize for 30 minutes every time The specimen clamp semiconductor refrigerating function is enabled or disabled Defrosting at fixed time or manually Large freezing shelf could load 8 cassettes simultaneously Two running way manual and automatic Emergency stop function Press the red button to stop running when any emergency happens 2 3Technical Parameter Environment requir...
Page 10: ...ents 20 to 100µm in 5µm increments Trimming thickness range 10 to 600µm 10 to 50µm in 5µm increments 50 to 100µm in 10µm increments 100 to 600µm in 50µm increments Retraction 0 80µm in 5µm increments Specimen feed 15mm 0 2mm Vertical stroke 48mm 1mm Specimen Max 35 35mm Repositioning of blade holder base left right 50 1mm Motorize cutting speed 75 230mm s 10 Dimension Length 805mm Width 715mm Heig...
Page 11: ... ventilation and heat dissipation Ensure the working temperature and humidity must be in accord with the specification in manual Ensure leaving enough running space for rotating the handwheel Notice Do not operate the instrument in the room with explosion hazard 3 2 Standard Delivery Cryostat Microtome 1 set Blade Holder 1 set Hand wheel 1 pc Specimen Clamp 10 pcs M5 Allen Key 1 pc M3 Allen Key 1 ...
Page 12: ...er Support 2 6 Corner Support 3 7 Handwheel 8 Blade Holder 9 Carton Base 10 Cryostat Microtome 11 Corner Support 4 12 Remove the cover 1 and carton body 4 then take the following things in order Upholder 2 Corner Support 1 3 Corner Support 2 6 Corner Support 3 7 Corner Support 4 12 Handwheel 8 Blade Holder 9 Cryostat Microtome 11 Glass Support 5 Then take out other accessories in the inner box pac...
Page 13: ...l lever 14 into handwheel 13 with 7mm wrench Insert bearing 15 handwheel cover 16 bearing 15 and handwheel washer 17 in order Tighten them with M5 X 16 hexagon socket screw 18 3 3 3 Blade holder Take out the Blade holder 19 from the box push it along the track as it is showed in the Fig and then rotate the Blade holder lock lever 20 to lock the blade ...
Page 14: ... need to replace it please use the power cord with earth wire Insert the fuse 23 into the fuse socket 22 as it showed in the Fig and insert the whole fuse socket into the socket 21 Attention Before changing the fuse please cut off the power supply and pull the plug Use the fuse according to the specification in operation manual As Fig shows power switch Turn off as the left fig and turn on as righ...
Page 15: ...rea show main information such as section thickness trimming thickness retraction cryo chamber temperature specimen clamp temperature and time The area is to set the value of section thickness trimming thickness retraction temperat ure and time Setting Key Indicator light RUN Auto running indicator light LOCK Locking light for hand wheel Defrosting light SECT TRIM Alternative select key RETRA Retr...
Page 16: ...o activate setting of the section trimming thickness retraction temperature and time Indicator light on Section mode RUN When this light is lit up it means this instrument is under auto running mode LOCK if its led is lit up it shows the handwheel is locked and it is disabled at this moment to slice section if its led lit up it shows it is defrosting now SECT TRIM is the alternating key between se...
Page 17: ...on ZZ is the sleep time indicator light The sleep time setting is ready when the sleep time light is on WAKE UP is the wake up time indicator light The wake up time setting when the wake up time light is on is the key to start defrosting immediately Hold on this button for about 5 seconds to start defrosting immediately Meanwhile the defrosting indicator light is on The process lasts about 30 minu...
Page 18: ... Fig the keyboard has been locked All other keys are disabled at this moment and release the lock by long press the button 4 1 2 Left control panel Fast forward and fast backward key Adjust the specimen position rapidly the speed could reach 900 30µm s Slow forward and fast backward key Adjust the specimen position slowly the speed could reach 300 30µm s Specimen feed distance is 15mm Once exceedi...
Page 19: ...omatic Select the mode by pressing this button shortly Autorun Stop button in motorized mode Press and hold this button about three seconds while the lights into autorun When the motorized mode starts the operation speed is slowest in default Press the button to stop running while goes out Speed adjusting button The button for adjusting speed of section can be activated only during running and the...
Page 20: ...11 anti roll plate height adjusting knob 12 Insert the longitudinal movable jaw 2 into the track of blade holder base 1 and rotate lever 2 8 to lock firmly Then adjust the handle of lever 2 8 to horizon position Adjust the rotating movable jaw 3 into longitudinal movable jaw 2 and rotate the lever 4 10 to lock firmly Put movable knife clamp 4 and fixed knife clamp 5 on rotating movable jaw 3 rotat...
Page 21: ...e as requirement before tightening it The rotating angle is from 0 to 10 degree see the picture the user can adjust the angle in this range according to the requirement Anti roll plate adjustment Anti roll plate is rectangular organic glass do as follows Insert the blade and rotate the fixed pole 7 to vertical position for tightening it Lock the two anti roll plate knobs 11 Adjust the bolts 15 wit...
Page 22: ...er 5 and push the blade 6 into blade holder 4 then rotate the lever 5 to clamp the blade It uses blade in the picture blade can be divided into two types low high profile If the high profile blade is needed twist the four screws 7 out with 3mm Allen key then take out anti roll plate module 8 twist out the two screws 9 on base plate 10 to take out the base plate 10 After inserting the blade refix t...
Page 23: ...mperature setting Press shortly and the line of temperature board area gets thicker When the display shows it has entered into cryo chamber temperature display status Then press to enter into temperature setting situation The value can be adjusted by as showed in left Fig After setting up press again to exit or leave it alone for 5 seconds to exit automatically The temperature range is between 50 ...
Page 24: ... as in left Fig its working time is 15 minutes the specimen clamp peltier function is able to be activated when the temperature of cryo chamber is below 8 C 4 5 Time setting 4 5 1 Real time setting Press shortly and the line of time board area gets thicker When the light is on which means the item real time has been selected as in left Fig press to enter into real time setting The value can be set...
Page 25: ...r into disinfection time setting The value can be set through Press to set minute after hour setting then press again to set week After finishing the disinfection time setting press to enter into sleep time setting or leave it alone for 5 seconds to exit automatically When the disinfection is on O3 icon shown at left will be displayed above the time zone And the disinfection function is activated ...
Page 26: ... time which can not wake up automatically After finishing the sleep time setting press to exit or leave it alone for 5 seconds to exit automatically 4 6 Section Trimming setting Section setting Press SECT TRIM shortly and the line of section board area gets thicker When the Letter before progress bar becomes S which means it has enter into section situation press to enter into section setting The ...
Page 27: ...e edge for section When the blade is far away from specimen press to move the specimen clamp forward or backward quickly When the blade is close to the blade press to adjust the position slowly 修片值 进度条 Trimming thickness Progress bar Board area Counter Section in motorized mode Long press the button about three seconds while the LED illuminates and automatic to run Short press the button to stop t...
Page 28: ...rature the lower the temperature is the harder the tissue will be It needs work through practice to identify appropriate temperature for better quality section The sectioning temperature range of most fat free without formalin fixed is best between 13 and 23 3 To obtain high quality section please pay attention to the following aspects 1 Proper cryo chamber temperature selection 2 Correct section ...
Page 29: ...h is often touched when operation and position 2 which always contacts with the specimens These two positions especially position 2 are very easy to be polluted so need to be cleaned frequently to ensure normal operation Cleaning the blade holder Take down all the parts of blade holder as it is showed in the picture ad then clean all the parts separately especially the following easily polluted pa...
Page 30: ...e cleaning Open the glass after turning off the instrument to keep the chamber dry Do not use any corrosive solvent for cleaning Ensure no liquid enters the interior of the instrument during cleaning Keep cleaning the cryo chamber frequently Do not turn on the instrument before it is completely dry 5 2 Maintenance Fuse replacement Put the fuse 1 in the installation part 2 and then insert the insta...
Page 31: ... section angle of the blade is too small The clamping setting is not steady The space between anti roll plate and blade is too small or anti roll plate is lower than the blade The temperature of blade or anti roll plate is too high The blade or anti roll plate is polluted Temperature too low for tissue cut Reconnect the main cable or replace it Replace the fuse If it is not matched please call for...
Page 32: ...e microtome The blade produces sounds when section and the section are scratched and show vibrated mark The gradient of the blade is not proper It is effected by static The gradient of the blade is not proper Readjust the gradient Increase the surrounding humidity o get rid of the static Reinstall the blade and adjust the gradient of the blade If there any other troubles can not be resolved please...
Page 33: ...ryostat Microtome 1 set 2 Blade Holder 1 set 3 Handling wheel 1 pc 4 Specimen clamp 10 pcs 5 M5 Allen Wrench 1 pc 6 M3Allen Wrench 1 pc 7 7mm Wrench 1 pc 8 Disposable blade 1 box 9 Power cord 1 pc 10 Brush 1 pc 11 Fuse 2 pcs 220V 8A 110V 15A 12 Operation Manual 1 pc ...
Page 34: ...Published by AMOS SCIENTIFIC PTY LTD 7 Gabrielle Court Bayswater North VIC 3153 Melbourne Australia Tel 61 410190188 Fax 61 410190188 ABN 37 159 778 140 www amos scientific com ...
