Amersham Biosciences Hoefer DALT, User Manual, Page: 46 / 70

Recipes Hoefer DALT System
42
Amersham Biosciences
10% TEMED
Displacing Solution
(0.375 M Tris-Cl, pH 8.8, 50% glycerol, bromophenol blue, 200 ml)
Water-saturated n -Butanol
Gel Storage Solution
(0.375 M Tris-Cl, pH 8.8, 0.1% SDS, 2.0 liters)
Final Conc. Amount
TEMED (v/v, MW 116.2) 10% 0.5 ml
Water 4.5 ml
Prepare fresh, in glass vessel.
Amount
Tris-Cl (1.5M, pH 8.8) 50 ml
Glycerol 100 ml
Bromophenol blue 2 mg
Water 50 ml
Prepare fresh. Stored solution may support microbial growth.
Amount
n -butanol 50 ml
Double-distilled water 5 ml
Combine in a bottle and shake. Use the top phase to overlay gels. Store at room temperature
indefinitely.
Final Conc. Amount
1.5 M Tris-Cl, pH 8.8 500 ml
10% SDS 0.1% 20 ml
Double distilled H
2
O to 2000 ml
Store at 4 °C.