Device User’s Guide
4.
Instrument setting for absorbance / transmittance measurement
19
①
First, set the spectrometer and light source as shown in Figure4-1. Connect the fiber
collimator to the cuvette holder and connect it to the spectrometer. Fix it with
platform screws on the platform. Connect the SMA905 adaptor for light shielding to
the side of the cuvette holder. When placing the light source on the platform, fix with
the platform screw at the position where the light irradiation port is closest to the
cuvette holder.
②
Turn on the light source and turn on the D2 and halogen lamps. Wait from 20 to 30
minutes until the light source stabilize.
③
Open the SpectraSmart software, and then open the shutter, set the cuvette
containing solvent or gas to the cuvette holder, and acquire the reference spectrum
(
※
)
.
④
While setting the cuvette, close the shutter (do not turn off the light source),
and acquire the dark spectrum
(
※
)
.
⑤
Open the shutter and set the cuvette containing the sample to be measured
into the cuvette holder and perform the measurement.
※
For the acquisition method, please refer to SpectraSmart User's Guide.
Example: 2. Using thin film
Set the thin film in the cuvette holder and measure the absorbance and transmittance.
Configuration example
PC (sold separately)
SEC2021 Spectrometer
SEC2022 Deuterium halogen light source
SEC2023 Cuvette holder
SEC2024 Platform
Platform screw
Fiber collimator
SMA905 adaptor for light shielding
Figure 4-2: Absorbance/Transmittance measurement example thin film
