3
Getting Started
Sample Preparation and Loading
20
Agilent SureCycler 8800 Setup and User’s Guide
Sample Preparation and Loading
WA R N I N G
To prevent damage to the block and the heated lid, use only
temperature- stable sample tubes and plates. Unsuitable tubes and plates
can become damaged during cycling. See
on page 21 for a list of recommended plasticware.
To prepare samples
Keep the following guidelines in mind when preparing samples in reaction
plates and tubes:
•
Place caps on tubes before loading samples into the thermal block.
•
For best results, just before loading the samples into the thermal block,
spin them briefly in a centrifuge.
•
If using sealing film to cover a reaction plate, use a compression mat
during cycling to ensure the film stays firmly adhered to the plate. See
“Agilent Plasticware and Consumables”
on page 21 for ordering
information on a compression mat.
To load samples
The instrument can be loaded with individual PCR tubes or strip tubes or,
depending upon the thermal block installed, one 96- well PCR plate or one
384- well PCR plate.
1
Lift the lid of the thermal block by pressing firmly on the button on the
top of the instrument to unlatch the lid.
2
Put your plate or tubes on the block and check that they are correctly
positioned.
On the 96- well block, seat the tubes in the round holes on the thermal
block. On the 384- well block, seat the tubes in the larger round holes.
3
Close the lid until it latches into place.
Danger of Burns: The thermal block, sample tubes and plates may reach
temperatures as high as 100°C. Keep hands away until temperature is 30°C or less.
Summary of Contents for SureCycler 8800
Page 1: ...Agilent Technologies SureCycler 8800 Setup and User s Guide Version A March 2011...
Page 4: ...4 Agilent SureCycler 8800 Setup and User s Guide...
Page 8: ...8 Agilent SureCycler 8800 Setup and User s Guide Contents...
Page 64: ...5 Maintenance and Updates Software Updates 64 Agilent SureCycler 8800 Setup and User s Guide...
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