Agilent Technologies BioCel 1200 User Manual Download Page 65

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Chapter 4: Performing a run

BioCel User Guide

Performing a run

This chapter describes the hardware procedures associated with
performing a run. All of the procedures in this chapter can be performed
by someone with operator privileges.

This chapter contains the following topics:

❑

“Pausing and stopping a run” on page 60

❑

“Comparing interlock and emergency circuits” on page 62

❑

“Monitoring the status of a run” on page 63

❑

“Cleaning up after a run” on page 65

❑

“Turning Off the BioCel” on page 66

Summary of Contents for BioCel 1200

Page 1: ...Agilent Technologies BioCel System User Guide...

Page 2: ...and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Te...

Page 3: ...ort 1 800 979 4811 or 1 408 345 8011 service automation agilent com Customer Service 1 866 428 9811 or 1 408 345 8356 orders automation agilent com European Service 44 0 1763853638 euroservice automat...

Page 4: ...Letter to our Customers 2 Agilent Technologies Inc Automation Solutions Division...

Page 5: ...nections 19 Power panel 21 Hardware control components 22 Power system 27 Air system 30 Vacuum system 31 Computers and networking 32 Adjusting the keyboard shelf 33 Docking the mobile device table to...

Page 6: ...66 Chapter 5 Diagnostics 69 About Diagnostics software 70 Using Bio I O Diagnostics 71 Using Robot Diagnostics 72 About fine tuning robot teachpoints 79 Fine tuning a robot teachpoint 83 Chapter 6 Ma...

Page 7: ...ontains the following topics Who should read this guide on page 2 What this guide covers on page 3 What is new in this version of the user guide on page 4 Accessing Velocity11 user guides on page 5 Re...

Page 8: ...erials and standard operating procedures for operators Operator Someone who performs the daily production work on the BioCel and solves routine problems Your organization may choose to create its own...

Page 9: ...n This guide covers the hardware for the BioCel 1200 1600 and 1800 This includes accessories such as the Weigh Pad and plate hotel BioCel customization Because the BioCel is a custom product with many...

Page 10: ...1600 BioCel 1600 and 1800 external features on page 18 Modifications to the BioCel 1600 Changes in the power panel Changes to the air panel Power panel on page 21 Hardware control components on page 2...

Page 11: ...ny PDF file from our website at www velocity11 com All Velocity11 user documentation can be searched from the website at www velocity11 com Online help The online help is the best format to use when y...

Page 12: ...use the viewer that is built into Adobe Acrobat or you can download the free Adobe Reader application from http www adobe com support downloads main html Printing and searching We provide user guides...

Page 13: ...om Calling Velocity11 Technical Support at 1 800 979 4811 or 1 650 846 6611 Reporting user guide problems If you find a problem with this user guide or have suggestions for improvement please use the...

Page 14: ...ty information topic of the user guide Chemical hazards Some chemicals used when working with the BioCel might be hazardous Make sure you follow the recommendations in the MSDS Material Safety Data Sh...

Page 15: ...to have the braces adjusted Overhead door damage hazard The overhead door hazards apply only if you have a BioCel 1600 or 1800 that has overhead doors DAMAGE HAZARD Always open and close overhead door...

Page 16: ...be moving With the interlock overridden it is possible to be hit by the robot when it is moving If this happens the robot will immediately stop minimizing injury and the likely outcome is minor bruis...

Page 17: ...of 60 centered perpendicularly to the reader With these measurements you can establish the space that is illuminated on occasions when there is no plate in the robot gripper INJURY HAZARD Class II las...

Page 18: ...Chapter 1 Introduction BioCel User Guide 12...

Page 19: ...17 BioCel 1600 and 1800 external features on page 18 Utilities connections on page 19 Power panel on page 21 Hardware control components on page 22 Power system on page 27 Air system on page 30 Vacuum...

Page 20: ...ry BioCel includes A frame A table A robotic arm A controlling computer a monitor a keyboard and a mouse A main power panel and electrical supply circuits Communication hubs and cables A Bio I O conso...

Page 21: ...ding Amendment 2 1995 Environmental specifications Dimension 1200 no hood 1600 with hood 1800 with hood Height 196 8 cm 77 5 in 259 1 cm 102 in 259 1 cm 102 in Width 205 7 cm 81 in 152 4 cm 60 in 182...

Page 22: ...about See BioCel features Basic description on page 14 BioCel 1200 external features on page 17 BioCel 1600 and 1800 external features on page 18 BioCel systems Power system on page 27 Air system on p...

Page 23: ...might be different Related topics 00242 Biocel 1200 00242 Interlock sensor Enclosure panels Monitor Keyboard Power panel Side door Foot Frame post External connections For information about See BioCe...

Page 24: ...ents on your BioCel may be different Related topics 00002 External connections Power panel Overhead door Foot Side door Status light Monitor Keyboard Frame post Hood with lights For information about...

Page 25: ...onnect into the BioCel behind the side doors These connections are visible when you open the side doors on the BioCel The illustration below shows where the utilities are connected behind the side doo...

Page 26: ...ect plumbing Any BioCel can be customized to have direct plumbing from an external water source In this case the water lines enter through the frame corners Related topics 00001 Main air line Ethernet...

Page 27: ...e power panel BioCel 1200 Control Panel 00243c INTERLOCK RESET AIR 0 0 MAIN POWER EMERGENCY STOP 00243c 0 0 WARNING BioCel 1200 ROBOT HOOD LIGHTS ON OFF Reset button Interlock keyhole Air switch Robot...

Page 28: ...ernet hub RS 232 hub Optional components include the following Bar code power supply Weigh Pad hub Pump modules The placement and in some cases the physical attributes of these control components depe...

Page 29: ...ator Controls how firmly the robot grips a plate Air manifold Houses air supply connectors for the BioCel devices Main air line Directs the external air source Main shutoff valve Controls the air supp...

Page 30: ...r they might be towers The model used depends on what other devices and equipment are on your BioCel The illustration below shows the two possible models Communication hubs The Ethernet and RS 232 hub...

Page 31: ...optional trash and enclosure doors Provides 24V power to some devices such as the Lid Hotel and Vacuum Delidder Many of the thin gray low voltage cables that run through the BioCel go to the Bio I O Y...

Page 32: ...pump modules may be mounted inside a side door underneath the BioCel deck Here they do not take up deck space and are easily accessible for maintenance Related topics 00255 BioCel Reader Power Supply...

Page 33: ...ule Device Monitor PC RS 232 hub UPS Robot DC power supply Robot Bio I O console Device Post emergency stop power strip Emergency stop relay Pre emergency stop block AC power in Cooling fans Main swit...

Page 34: ...are also not buffered by the UPS from power outages UPS connections If there is more than one UPS they are connected in series so that current output from UPS 1 supplies the current input for UPS 2 Th...

Page 35: ...t be pushed to reset the emergency stop relay to a position that allows current to flow The door interlock system is fed AC electricity from the emergency stop relay If an overhead door is opened duri...

Page 36: ...rs Many devices have their own built in regulators Air pressure to other parts is controlled by regulators in the air panel of the BioCel The diagram above shows how air to the BioCel can be cut off d...

Page 37: ...rd party devices The vacuum can be supplied by a pump or house vacuum system If a house vacuum system is used the main vacuum line enters the BioCel with the other external connections If a pump is us...

Page 38: ...ate readers have their own computer for storing data Communicate with other computers on your organization s network for the exchange of files reporting of errors and accessing email Network cards If...

Page 39: ...pivot joints to make the adjustments Use the labeled illustration below to locate the appropriate joint for adjusting 2 To loosen or tighten the joints pry off the joint covers and use a wrench to mak...

Page 40: ...d shaft are all permanently mounted to the mobile table Two docking slots that receive the alignment rods and a T nut that receives the threaded shaft are permanently mounted on the BioCel The followi...

Page 41: ...re many third party devices that can be used on a BioCel For a comprehensive list see the Device Driver User Guide If there is product that you would like to add to your BioCel please contact Velocity...

Page 42: ...on page 14 For information about See Accessory Description and Comments Auxiliary bar code reader One or more bar code readers attached to a platepad or other device Environmental Enclosure Glass enc...

Page 43: ...e used with the VWorks inventory management system For information about setting up and using the VWorks inventory management system see the VWorks User Guide Transfer station The transfer station is...

Page 44: ...The vacuum based lid remover sits over the waste opening in the table It uses one or more suction cups to remove plate lids The vacuum used to hold the lids is created by a vacuum ejector which uses a...

Page 45: ...ept developed by Novartis Pharma AG NIBR DT IAT Basel Switzerland VShuttle The VShuttle has two decks each with a pair of rotating plate pads It allows plates to move between robots while maintaining...

Page 46: ...rent sizes They report the percentage of liquid in a container that sits on the Weigh Pad Related topics 00110 Weighpads For information about See Adding and removing plates from the Plate Hotel Placi...

Page 47: ...is chapter contains the following topics Workflow for preparing the BioCel for a Run on page 42 Turning on the BioCel on page 43 Performing a BioCel ready state check on page 45 Preparing devices and...

Page 48: ...ng a ready state check Performing a BioCel ready state check on page 45 7 Preparing labware and accessories Preparing devices and accessories on page 46 8 Preparing the plate hotel Placing and removin...

Page 49: ...button The UPS LEDs illuminate to indicate the UPS power status If you have more than one UPS on your BioCel make sure you turn on all of them If the UPSs are connected in series turn on the UPS that...

Page 50: ...s open and close overhead doors using their handles Opening and closing overhead doors by pulling and pushing on their corners may crack the glass and damage the pneumatic braces 9 Press the green res...

Page 51: ...ave status lights All devices are on pneumatic devices are supplied with air and a vacuum is supplied where used The computer is on and ready for a user to log in to the operating system The UPSs are...

Page 52: ...herefore be set up the night before the run Leave the more complex preparations which might use expensive and unstable reagents until last Note If the protocol includes User Message tasks to prompt yo...

Page 53: ...waste bin from its hanger 1 Open the side door nearest the bin 2 Hold the bin at the top right corner with your right hand 3 Lifting the front side of the bin upwards with your right hand place your...

Page 54: ...iver plates according to the protocol you will run As the racks can be positioned either way round make sure that the A1 wells of the plates are in the top left corner as viewed by the robot 5 After o...

Page 55: ...lose the VSpin door If you are using robot loaded counterweights make sure you have correctly set up counterweights in VWorks placed the counterweights in the correct plate positions and checked to ma...

Page 56: ...dowel pins and hotel bases Removing and replacing a hotel IMPORTANT If you remove a plate hotel you must put it back in the same place to maintain accurate teachpoints To remove a plate hotel 1 Holdi...

Page 57: ...n time is 45 seconds or longer the protocol will probably run fastest if you load two protocol plates and don t use a counterweight Options for loading plates A counterweight should be the same weight...

Page 58: ...match the type of sample plate Note The administrator who creates a protocol can add user messages to the protocol to remind the operator before a run which types of plate to put on which platepad Se...

Page 59: ...r head This button turns on the laser creating the possibility of a long exposure to the light Procedure To align and test a bar code reader 1 Place a plate labeled with a bar code on the plate pad th...

Page 60: ...ide the rack out 3 Fill the racks and slide them back into position 4 Make sure rack locations and other PlateHub parameters are set correctly in the PlateHub Diagnostics Related topics For informatio...

Page 61: ...Platepads VPrep shelves VStack plate stages VSpin buckets VCode plate stages PlateLoc plate stages Plate hotel shelves PlateHubs Lid hotel bays Lid removal devices Third party devices You can also se...

Page 62: ...ware 6 For each VStack open the VStack BioNet Diagnostics software to check that the grip pressure is appropriate for the type of plates that you are using For more information about adjusting the gri...

Page 63: ...57 Chapter 3 Preparing for a run BioCel User Guide Setting the robot to check all accessible plate positions VWorks User Guide For more information about See...

Page 64: ...Chapter 3 Preparing for a run BioCel User Guide 58...

Page 65: ...run All of the procedures in this chapter can be performed by someone with operator privileges This chapter contains the following topics Pausing and stopping a run on page 60 Comparing interlock and...

Page 66: ...at will cause an error or cause samples to be switched around when the run continues For example errors can be caused when manually moving a plate to a location that should not have a plate Stopping a...

Page 67: ...a robot error after which you can either continue or abort the run or enter diagnostics When you close the door an error dialog box opens If a VPrep was in motion when the door was opened a VPrep Erro...

Page 68: ...ency stop The following table shows how different components are affected by the door interlock circuit and emergency stop circuit Related topics Component Interlock cuts Emergency stop cuts Bio I O p...

Page 69: ...displayed Add and remove labware Empty the waste bin Empty liquid waste containers Fill liquid reservoirs Replace an empty roll of PlateLoc seal or bar code labels IMPORTANT No errors are reported wh...

Page 70: ...l conditions Light Pattern Status All on solid Run progressing normally All off Not currently running All blinking simultaneously Run error One light on each post blinking twice followed by the other...

Page 71: ...such as reservoirs tubes and manifolds that were contacted by liquid 5 Empty the waste bin 6 Check run logs Red Error During operation an error occurred and the system cannot continue without user int...

Page 72: ...ure you have performed a clean up after the last run 2 Exit VWorks 3 If you have a secondary computer a Exit any software running b Shut down the secondary computer through Windows Wait for the operat...

Page 73: ...ion about turning off the UPS see the user manual for the UPS 10 On the power panel turn the main power switch counterclockwise to the off position 11 If moving the BioCel unplug the air Ethernet and...

Page 74: ...Chapter 4 Performing a run BioCel User Guide 68...

Page 75: ...ices see the user guide for the device For information about how to use the diagnostics software for third party devices and Velocity11 devices not sold as standalone products see the Device Driver Us...

Page 76: ...VStack stage out of its home position you can use Robot Diagnostics to move the plate and VStack Diagnostics to return the VStack stage to its home position Types of diagnostics software Instruments a...

Page 77: ...Diagnostics This topic describes how to open the Bio I O Diagnostics in VWorks3 and test an output If you are running VWorks4 the procedure might be different Procedure To use the Bio I O Diagnostics...

Page 78: ...peed of robot movement 1 In the Robot Speed area select Slow Medium or Fast Note We recommend using the Slow or Medium setting for manual control For information about See What the Bio I O does Hardwa...

Page 79: ...n move the robot in increments in either the z axis r axis or t axis The process of moving in increments is called jogging DAMAGE HAZARD Before you jog the robot make sure that there is nothing on the...

Page 80: ...rely need to change this value from the values automatically entered for the plate type However you can type a value into the Gripper offset text box if you need to Be aware however that this is a tem...

Page 81: ...t coordinates of 0 0 0 Provided the home coordinates have not changed this position is the same as the home position Resting the robot is faster than homing the robot because it moves at the speed se...

Page 82: ...h 5 Click Pick Place 1 2 The robot moves to Position 1 and picks up the plate The bar code is read and displayed in the Bar Code Testing group box Ignoring robot errors You have the option of ignoring...

Page 83: ...re is a plate in the gripper the plate might contact a lip on the device causing a robot crash Also if there is a plate at the position and the robot grippers are closed there will be a robot crash Mo...

Page 84: ...Settings 1 Type the new value into the text box for the parameter you want to change using the following table as a guide Parameter Description Robot COM port The communications port to which the rob...

Page 85: ...ever you might occasionally need to fine tune teachpoints For information about device teachpoints see the relevant user manual This topic covers only robot teachpoints Robot teachpoints defined When...

Page 86: ...on is 1 mm less than the optimum the end result is that the plate is placed accurately at the position Also if the teachpoint of the picking position has an r value that is low by 1 mm and the teachpo...

Page 87: ...diagram the teachpoint is incorrect The back of the robot gripper stops before aligning with the back of the teachplate calibration tool and the teachplate is held too far back in the gripper The resu...

Page 88: ...an incorrect z teachpoint Related topics 00260 Incorrect Teachpoint 00260 Optimal teachpoint Back of plate 00262 Z off Teachpoint 00262 For information about See Fine tuning procedure Fine tuning a r...

Page 89: ...ures of this software before working with teachpoints Before you start Before you start make sure you have a teachplate calibration tool to use for the fine tuning Procedure To fine tune a robot teach...

Page 90: ...n to the sounds it makes If the plate catches the lip of the plate position because the r or t value is incorrect it can make a double click sound If the z value is incorrect the plate will make a dro...

Page 91: ...in good working order and what to do when you encounter a problem This chapter contains the following topics Routine maintenance on page 86 Resolving robot errors on page 87 Overriding the safety int...

Page 92: ...1 Open the side door behind the air panel 2 Look at the front of the air filter container If there are any oil droplets or general dirt visible on the filter the filter should be changed Contact the V...

Page 93: ...VStack to another 2 Close the overhead door that was opened 3 Make sure that the interlock light on the power panel is on 4 In the Robot Error dialog box select Retry If you are an administrator you c...

Page 94: ...t Error dialog box that is already open 5 Activate the interlock override by turning the key in the power panel 6 If the protocol includes a pipette task in the VPrep EStop detected dialog box click R...

Page 95: ...ly at the pickup location reposition the plate and click Retry in the Robot Error dialog box If the plate was positioned correctly at the pickup location there may be a problem with the approach or de...

Page 96: ...erride the safety interlock so you can perform tasks on the BioCel deck This topic describes how to temporarily override the safety interlock This procedure should only be performed by an administrato...

Page 97: ...ncluding approach height In this case see for more information about fine tuning teachpoints Device because it was moved or reconfigured by someone else who did not re teach the teachpoints Plate In t...

Page 98: ...Bar code labels are not applied to the lids connecting them to plates Stacks of labels are not allowed to build up on the plates The lids are free of sticky residue from labels Solving problems If yo...

Page 99: ...t which time VWorks closes and the computer performs an orderly shutdown Recommended actions during a power outage If you are running a protocol and are near the end you might consider letting it cont...

Page 100: ...Chapter 6 Maintenance and troubleshooting BioCel User Guide 94...

Page 101: ...otically loaded about 52 setting up robotic loading 52 D damage hazard computer monitor 9 overhead door 9 DC power supply 28 diagnostics software about 70 Bio I O 71 robot 72 disposing of waste 8 door...

Page 102: ...sensor checking 76 sensor errors 89 plate hotel 36 description placing and removing 50 PlateLoc 35 PlatePierce 35 power main supply 19 panel 21 pre emergency stop current 28 pre run checks performing...

Page 103: ...onnections 28 UV lights 28 V vacuum creating 30 system 31 VCode 35 VPrep 29 35 brakes 29 pumps 29 VSpin 35 VSpin counterweight see counterweight plate VStack 35 W warranty 8 waste bottle 31 disposal 8...

Page 104: ...Index BioCel User Guide 98...

Page 105: ......

Page 106: ...Agilent Technologies User Guide G5500 90002...

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