Agilent Technologies Agilent 1260 Infinity, System User'S Manual

Page: 79 / 140

1260 Infinity Binary LC - System User Guide 79
System Setup and Installation
4
Installing the Modules
Changing Solvents
1 If the channel is not filled with buffer, proceed to step 4.
2 Place the solvent intake filter into a bottle of water.
3 Flush the channel at a flow rate suitable for the installed tubing
(typically 3 – 5 mL/min) for 10 min.
4 Modify the flow path of your system as required for your application.
5
Replace the solvent bottle by a bottle of isopropanol.
6
Flush the channel at a flow rate suitable for the installed tubing
(typically 3 – 5 mL/min) for 5 min.
7
Swap the bottle of isopropanol with a bottle of solvent for your
application.
8
Repeat steps 1 to 7 for the other channel(s) of the pump.
9
Install the desired column, set the required composition and flow rate
for your application and equilibrate the system for approx. 10 minutes
prior to starting a run.
When When the solvent of a channel is to be replaced by another solvent that is not compatible (solvents
are immiscible or one solvent contains a buffer), it is necessary to follow the procedure below to
prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system.
Parts required # p/n Description
1 Purging solvent(s), see Table 8 on page 80
1 5022-2184 Union ZDV
Preparations Remove the column and replace it by a ZDV fitting
Prepare bottles with appropriate intermediate solvents (see Table 8 on page 80)
C A U T I O N
Buffer salt of aqueous buffers may precipitate in residual isopropanol.
Capillaries and filter may be clogged by precipitating salt.
➔
Flush solvent lines containing high concentration of salts first with water before
introducing organic solvent.
➔
Do not perform steps 5 to 7 for channels running with aqueous buffer as solvent.