1260 Infinity Binary LC - System User Guide
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Optimization of the Agilent 1260 Infinity Binary LC
3
How to Achieve Higher Sensitivity
Optimum Instrument Configuration for High Sensitivity
General Considerations
•
To achieve lowest baseline noise, the standard delay volume
configuration is recommended for the 1260 Infinity Binary Pump .
•
Here also, the injection volume and the sample dissolution solvent are
important. Care must be taken that the compounds are focused at the
top of the column, to avoid peak dispersion due to the injection, which
would cause a reduced peak height. In order to achieve this, the sample
should be dissolved in a solvent composition of lower elution strength
than the mobile phase.
•
The column temperature should not be too low, to avoid long retention
of the peaks on the column. This also creates peak dispersion and a
lower peak height.
•
The data rate of the UV detector should be selected according to the
actual peak width. Higher than necessary data rates should be avoided
because of higher noise levels.
•
Available UV detectors are the Agilent 1260 Infinity Diode Array
Detector (G4212B) and the Agilent 1260 Infinity Variable Wavelength
Detector (G1314F), with data rates of 80 Hz and significantly lower
noise and drift levels.
•
on page 63 gives an overview of the flow cells
available with the G4212B DAD . For information on flow cells used
with the G1314F VWD , please refer to the User Manual of the Agilent
1260 Infinity Variable Wavelength Detector .
Chromatographic Conditions
For an optimum signal- to- noise ratio, it is of advantage if the peaks elute
quickly.
•
Elevated flow rates should be used to elute the peaks at low k’values.
•
Faster gradients can also be applied to elute the peaks at low k’values.
