Agilent Technologies 5973N, Hardware Installation

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Installation
Hardware Installation 29
Conditioning the Column
1 Remove the septum covering the free end of the GC column
and cut about 1 cm off the end of the column.
2 Place the free end of the column into a beaker of solvent and
check for bubbles to verify helium flow. Do not leave the
beaker in the oven.
3 Set the injection port temperature to 300 °C.
4 Turn on the oven and ramp the oven temperature from 40 °C
to 300 °C (or 10 °C above the maximum temperature used for
your analysis) at 15 °C per minute. Hold for 10 minutes, then
reset to 35 °C.
While the oven is heating, proceed with the next steps.
5 Once the oven temperature exceeds 80 °C, inject 5 µL
methanol into GC; repeat two more times at 5-minute
intervals.
This will help remove any contamination from the column
before it is installed into the MSD.
6 When the temperature program is complete, set the injection
port to 250 °C.
7 Turn the GC power off.