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2D-LC User Guide
25
2
Concepts of 2D-LC
Multiple Heart-Cutting and High Resolution Sampling 2D-LC
High Resolution Sampling - Peak Parking Principles
In the
HiRes sampling
mode, the multiple heart-cutting (MHC) valve is switched
before and after
parking the peak. This has the following consequences:
•
Each loop for consecutive snips stores the same sample volume.
•
First and last loop cannot be used for parking.
•
Solvent transfer from
1
D to
2
D can be reduced.
•
Cut number 5 cannot be parked entirely in the sample loop. Otherwise cut 6
would got partially to the transfer capillary and would therefore be lost or spoil
cut 5.
Cut 5 stays partially in the transfer line and is immediately being analyzed in
2
D.
•
For parking cut 6 in the sample loop, the cut first needs to be moved from the
2D-LC valve to the deck valve.