7
Method Development of Active Solvent Modulation (ASM)
Include the ASM Phase to the 2D Gradient
2D-LC User Guide
190
Include the ASM Phase to the
2
D Gradient
Figure 103 Programming the
2
D gradient table (example)
The dilution during the ASM phase takes time. That's why the ASM phase shifts
the analytical gradient start.
An ASM phase of, for example, 0.68 min (based on selected ASM capillary, flush
factor and
2
D flow rate) shifts all times by 0.68 min compared to a
2
D gradient
without ASM
•
Gradient ends later
•
2
D cycle time is increased accordingly
•
One line is added to the gradient table for the ASM phase
•
All analytical gradient times shift by 0.68 min
This rule is true for shifted gradient steps as well (if applicable).
