2D-LC User Guide
179
6
Method Parameters
Additional Information
Dynamic Peak Parking
In certain cases, small variations of parameters can influence changes in the
retention time (RT) mechanism. This can happen, for example, with certain types
of analytes such as peptides. As a solution to compensate for such effects in
Time-based (M)HC 2D-LC experiments, the Dynamic Peak Parking is used.
“Dynamic Peak Parking” uses an internal RT-standard (IRTS), which is detected
by using peak-based mode. If the “expected time” of this IRTS shifts to earlier or
later, subsequent time-based cuts linked to the IRTS will be adjusted accordingly.
Setup an IRTS experiment for heart cutting mode
1
Upload the chromatogram into the preview.
2
Define the UV-threshold (peak trigger) such that the expected IRTS is
predicted to being sampled, see
How to setup the peak-based experiment
.
3
Define the peak-based area (start and end peak -based) in which the IRTS is
expected.
This can be done for instant via Sampling table or by selecting the icons peak
based plus/ MHC or HiRes in the preview UI and double clicking on the
triangle of the peak of interest (IRTS), see
How to setup the peak-based
experiment
4
If needed, adjust the peak-based area either by grabbing start and end bracket
and moving along in the preview or by adjusting the times in the sampling
table.
Figure 95
Peak-based heart cutting experiment
