6
Method Parameters
Set up a Peak-Based Experiment Graphically
2D-LC User Guide
168
Set up a Peak-Based Experiment Graphically
Figure 84
Peak-based experiment based on a prediction
In peak-based mode, the
1
D detector triggers sampling/parking of cuts in
dependence on a UV-threshold (or slope) A peak appears in the detector, the
threshold is reached (= peak start), the 2D-LC modulator starts sampling. A
sampling time can be defined, which determines the max. sampling time for
peak-based cuts. If peak-end is detected before the sampling time has finished,
sampling is stopped. The event that comes first will define the time for
peak-based sampling. Adjust the peak-based area either by grabbing start and
end bracket and moving along in the preview or by adjusting the times in the
sampling table.
1
Upload chromatogram into preview.
2
Define UV-threshold and mark threshold symbol
for display.
