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2
Concepts of 2D-LC
Concepts of 2D-LC
2D-LC User Guide
16
Concepts of 2D-LC
In a 2D-LC-System,
1
D pump generates the
1
D gradient. An autosampler injects
the sample and separates it by
1
D column. A 2D-LC Valve (Injector) connects the
first dimension to the second dimension and stores sample peaks
intermediately. These sample peaks are re-injected to the second dimension,
separated by a
2
D column and measured by the
2
D detector.
Figure 1
Concept of 2D-LC
Figure 2
Conceptual illustration of heart-cutting 2D-LC principle
D
1
D
2