30
Agilent 1260 Infinity II Preparative Autosampler User Manual
2
Site Requirements and Specifications
Performance Specifications
Performance Specifications
Table 2
Performance Specifications 1260 Infinity II Preparative Autosampler (G7157A)
Type
Specification
Comment
Settable injection
range
0.1 – 900 µL, up to 3600 µL with seat extension
Injection precision
1 µL:
<
5 %
5 µL:
<
2 %
10 µL, 50 µL:
<
1 %
500 – 3600 µL:
<
0.25 %
Measured caffeine
Pressure range
0 – 40 MPa (0 – 400 bar, 0 – 5801.51 psi)
Sample viscosity
range
0.2 – 5 cp
Sample capacity
132 x 2 mL vial (two trays default)
100 x 2 mL vial (two classic trays optional)
36 x 6 mL vials (two trays optional)
Carry over
<
0.005 % (50 ppm)
Method
•
Column: Agilent 5 Prep-C18, 21.2 mm x
50 mm, 5 µm (446905-102)
•
Eluent: Water/ACN (85/15) isocratic
•
Flow rate: 30 mL/min
•
Temperature: RT
•
Detection: 273/4 nm Ref. 360/100 nm,
10 Hz
•
Injection volume: 500 µL
•
Needle wash: 10 s in flush port with
Water/ACN (85/15)
•
Draw speed: 900 µL/min
•
Eject speed: 3000 µL/min
•
Wait time after draw: 5 s
Sample
•
Caffeine 5 g/L in Water /ACN (98/2)
Injection cycle time
<
60 s for injection volume 900 µL
