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1100 Series Nano Pump Service Manual

259

Specifications

7

Safety and maintenance

Extensive diagnostics, error detection and display (through control
module and Agilent ChemStation), leak detection, safe leak handling,
leak output signal for shutdown of pumping system. Low voltages in
major maintenance areas.

GLP features

Early maintenance feedback (EMF) for continuous tracking of
instrument usage in terms of seal wear and volume of pumped mobile
phase with user-settable limits and feedback messages. Electronic
records of maintenance and errors.

Housing

All materials recyclable.

Table 64

Performance Specification Agilent 1100 Series Micro Degasser

Type

Specification

Maximum flow rate

5 ml/min per channel

Number of channels

4

Internal volume

Typically 1 ml per channel

Internal design

One chamber per channel with optimized flow path for fastest solvent
change over

Material in contact with
solvents

PTFE, FEP, PEEK

Table 63

Performance Specification Agilent 1100 Series Nano Pump, continued

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Summary of Contents for 1100 Series

Page 1: ...s1 Agilent 1100 Series Nano Pump Service Manual ...

Page 2: ... document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in...

Page 3: ...aterials Detailed illustrations and lists for identification of parts and materials 5 Introduction to the Nano Pump An introduction to the nano pump instrument overview theory of operation external communication and internal connectors 6 Control Module Screens for the Nano Pump Introduction to the screens available for operation of the Agilent 1100 Series nano pump with the control module 7 Specif...

Page 4: ...iv 1100 Series Nano Pump Service Manual ...

Page 5: ...ling the Nano Pump 12 Installing the Nano Pump 13 Install the Degasser G1379A 15 Install the Solvent Cabinet 15 Get the System Ready for the First Injection 17 Manually Priming the Solvent Channels 17 Purging the Pump 18 2 Troubleshooting and Test Functions Status Indicators 23 Power Supply Indicator 23 Instrument Status Indicator 24 Error Messages Indication 24 Error Messages 25 Time out 27 Shutd...

Page 6: ...r Limit 41 Pressure Signal Missing 42 Valve Failed 43 Missing Pressure Reading 44 Pump Configuration 45 Valve Fuse 46 Inlet Valve Fuse 47 Temperature Out of Range 48 Temperature Limit Exceeded 49 Motor Drive Power 50 Encoder Missing 51 Inlet Valve Missing 52 Electro Magnetic Proportional Valve EMPV Missing 53 Flow sensor missing 54 Unsupported Flow Sensor 55 Leak Sensor Missing 56 Servo Restart Fa...

Page 7: ...lly Low 93 System Pressure Abnormally High 94 EMPV failed to initialize micro mode only 95 Unstable column flow and or system pressure 96 Poor peak shape 98 Failure to produce peaks or abnormally small peaks after injection 99 Wandering Detector Baseline 100 User interface displays error messages for specific modules 101 3 Repairing the Pump Introduction 105 Simple Replacements 105 Exchanging Inte...

Page 8: ...he EMPV Assembly 117 Exchanging the Solvent Selection Valve 118 Removing and Disassembling the Pump Head Assembly 120 Exchanging the Pump Seals and Seal Wear in Procedure 122 Exchanging the Plungers 125 Exchanging the Flow Sensor 126 Reassembling the Pump Head Assembly 127 Exchanging the Optional Interface Board 129 Exchanging Internal Parts 130 Removing the Top Cover and Foam 131 Exchanging the N...

Page 9: ... Bottle Head Assembly 160 Nano Pump Hydraulic Path 161 Pump Head Assembly 163 Electro Magnetic Proportional Valve EMPV 165 Flow Sensor Assembly 166 Power and Status Light Pipes 167 Leak Parts 168 Cover Parts 169 Sheet Metal Kit 170 Foam Parts 171 Nano Pump Accessory Kit G2226 68705 172 Control Module G1323B 173 Cable Overview 174 Analog Cables 176 Remote Cables 179 BCD Cables 184 Auxiliary Cable 1...

Page 10: ...204 Early Maintenance Feedback EMF 205 EMF Counters 205 Using the EMF Counters 206 The Electronics 207 Nano pump main board NPM 207 Firmware Description 213 Resident System 213 Main System 213 Firmware Updates 214 Optional Interface Boards 215 BCD Board 215 LAN Board 217 Interfaces 218 Analog Signal Output 219 GPIB Interface 219 CAN Interface 219 Remote Interface 220 RS 232C 221 Setting the 8 bit ...

Page 11: ...lable from the Analysis view 233 Settings screens 236 Method screens 244 Screens available from the System view 245 Screens available from the Records screen 248 Diagnostics and Tests 254 7 Specifications Performance Specifications 258 A Safety Information General 262 Operation 262 Safety Symbols 264 Lithium Batteries Information 265 Danish Information 265 Radio Interference 266 Test and Measureme...

Page 12: ...xii 1100 Series Nano Pump Service Manual Agilent Technologies on Internet 269 Index 271 ...

Page 13: ...ano Pump Service Manual Agilent Technologies 1 Installing the Pump Site Requirements 2 Unpacking the Nano Pump 5 Optimizing the Stack Configuration 7 Installing the Nano Pump 12 Get the System Ready for the First Injection 17 ...

Page 14: ...rds are offered as options with the nano pump The female end of each of the power cords is identical It plugs into the power input socket at the rear of the nano pump The male end of each of the power cords is different and designed to match the wall socket of a particular country or region WARNING To disconnect the nano pump from line unplug the power cord The power supply still uses some power e...

Page 15: ...e humidity as described in Table 1 WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations NOTE The pump should be operated in a horizontal position CAUTION Do not store ship or use your nano pump under conditions where temperature fluctuations could cause condensation within the nano pump Condensati...

Page 16: ...Maximum Ambient operating temperature 4 55 C 41 131 F Ambient non operating temperature 40 70 C 4 158 F Humidity 95 at 25 40 C 77 104 F Non condensing Operating Altitude Up to 2000 m 6500 ft Non operating altitude Up to 4600 m 14950 ft For storing the nano pump Safety standards IEC CSA UL Installation Category II Pollution Degree 2 Table 1 Physical Specifications continued ...

Page 17: ...sure all parts and materials have been delivered with the nano pump For this compare the shipment content with the checklist included in the instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office Accessory Kit Each shipment contents an accessory kit with the necessary tools to install the pump and to have an operating system CAUTION If the...

Page 18: ...ey 4 0 mm 15 cm long T handle x 1 8710 2392 Torque adapter G1315 45003 CAN cable 1 m long 5181 1519 Purge valve assembly G1311 60009 Purge valve holder G1312 23200 Screw for the purge valve holder 0515 0175 FS to Inj valve cap 350 mm 25 µm G1375 87322 FS to Inj valve cap 550 mm 25 µm G1375 87323 Flow sensor accuracy calibration capillary 8000 mm 25 µm G2226 67300 ESD wrist strap 9300 1408 Table 2 ...

Page 19: ... the following configuration This configuration optimizes the system flow path ensuring minimum delay volume NOTE If a single stack configuration becomes too high e g if an additional module like a G1330B ALS Thermostat is added or if your bench is too high a two stack configuration may be a better setup Separate the stack between pump and sampler and place the stack containing the pump on the rig...

Page 20: ...8 1100 Series Nano Pump Service Manual 1 Installing the Pump Figure 1 Recommended Stack Configuration Front View Solvent cabinet Vacuum Pump Column compartment Well plate sampler Detector Control module ...

Page 21: ...ies Nano Pump Service Manual 9 Installing the Pump 1 Figure 2 Recommended Stack Configuration Rear View GPIB or LAN to LC Analog signal to recorder Remote cable CAN Bus cable AC power Analog signal to recorder ...

Page 22: ...0 Series Nano Pump Service Manual 1 Installing the Pump Figure 3 Recommended stack configuration front view Autosampler ALS Thermostat Column Compartment Detector Control Module Pump Degasser Solvent Cabinet ...

Page 23: ...nual 11 Installing the Pump 1 Figure 4 Recommended stack configuration rear view GPIB or LAN to LC ChemStation Analogsignal to recorder AC power Remote cable CAN bus cable Autosampler Thermostat cable AC power Analogsignal to recorder ...

Page 24: ...her cables see text below and Cable Overview on page 174 ChemStattion and or Control module G1323A B WARNING When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous ...

Page 25: ...he power socket at the rear of the pump Do not connect the power cable to power until you have finished the hardware installation of all modules in the stack 3 Connect the free end of the Can bus cable from the micro sampler to one of the Can bus connectors at the rear of the pump Figure 5 Rear of Nano Pump GPIB Security Lever Configuration switch Power Plug RS232 Remote Analog output Slot for int...

Page 26: ...le can be connected to the CAN bus at any of the modules in the system except for the degasser The Agilent ChemStation can be connected to the system through one GPIB or LAN requires the installation of a LAN board cable at any of the modules except for the degasser preferably at the detector MUST for the DAD For more information about connecting the control module or Agilent ChemStation refer to ...

Page 27: ...er Connect the other end of the solvent tube to its intended port at the pump solvent selection valve Follow the guide below Install the Solvent Cabinet 1 The solvent cabinet accessory kit has 4 bottle head assemblies G1311 60003 2 Connect a bottle head assembly to each of the degasser INLET ports Use the labels provided with each bottle head assembly to appropriately label each bottle head assemb...

Page 28: ...ection of the capillary pump Table 3 Connecting capillaries for the Nano Pump Item Part Number 1 G1311 67304 2 G1312 67300 3 G1312 67302 4 G1312 67304 5 01090 87308 6 G1375 87400 7 220 mm 25 µm G1375 87321 8 350 mm 25 µm G1375 87322 8 550 mm 25 µm G1375 87323 1 1 2 2 3 3 4 5 6 7 8 ...

Page 29: ...ttles and install the bottles on the desired solvent channels Install Isopropanol on channels which will not be used right away 3 Put a paper towel over the leak sensor in the pump leak tray 4 Disconnect the channel A solvent tube from the A1 port of the pump solvent selection valve WARNING When opening capillary or tube fittings solvents may leak Please observe appropriate safety precautions such...

Page 30: ...tem All system parameters should be set to default The degasser should also be turned on at this time 3 Initialize the system Then access the pump controls and make sure the pump mode is set to Normal 4 Access the pump Purge control Set up a purge table which will purge all channels for 5 minutes each at a flow of 2500 µl min Then start the purge NOTE When the pump has been turned off for a certai...

Page 31: ...er a solvent change Bidistilled water Best solvent to re dissolve salts After the installation of normal phase seals P N 0905 1420 Hexane 5 Isopropanol Good wetting properties To clean the capillaries Acetone Best solvent to remove impurities from the capillaries Table 4 Choice of Priming Solvents for Different Purposes continued Activity Solvent Comments ...

Page 32: ...20 1100 Series Nano Pump Service Manual 1 Installing the Pump ...

Page 33: ...r Messages In the event of an electronic mechanical or hydraulic failure the nano pump generates an error message in the user interface The following pages describe the meaning of the error messages For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided Error Messages on page 25 Micro Pressure Tes...

Page 34: ...test to help troubleshoot the nano pump and its pumping performance Leak Test on page 73 EMPV Test The test is designed to verify the performance of the EMPV The test must always be done when the EMPV valve is exchanged The test should also be done if column flow stability problems occurs in micro mode see The EMPV test is not a substitute for the leak test or pressure test The leak and pressure t...

Page 35: ...per right one indicates the instrument status Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON When the indicator is off the module is turned off Otherwise check power connections availability of power or check functioning of the power supply Figure 7 Location of Status Indicators Status indicator Pow...

Page 36: ...nternal problem which affects correct operation of the instrument Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis A flashing yellow status indicator indicates that the module is in its resident mode Call your local service provider for assistance upon observing this error condition A flashing red status ...

Page 37: ...nchronization Lost 30 Leak 31 Leak Sensor Open 32 Leak Sensor Short 33 Compensation Sensor Open 34 Compensation Sensor Short 35 Fan Failed 36 Open Cover 37 Restart Without Cover 38 Zero Solvent Counter 39 Pressure Above Upper Limit 40 Pressure Below Lower Limit 41 Pressure Signal Missing 42 Valve Failed 43 Missing Pressure Reading 44 Pump Configuration 45 Valve Fuse 46 Inlet Valve Fuse 47 Temperat...

Page 38: ...oting and Test Functions Flow sensor missing 54 Unsupported Flow Sensor 55 Leak Sensor Missing 56 Servo Restart Failed 57 Pump Head Missing 58 Index Limit 59 Index Adjustment 60 Index Missing 61 Stroke Length 62 Initialization Failed 63 Wait Time out 64 ...

Page 39: ...sis was completed successfully and the time out function switched off the nano pump as requested A not ready condition was present during a sequence or multiple injection run for a period longer than the time out threshold Suggested Actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required ...

Page 40: ...cted in another module with a CAN connection to the system Leak detected in an external instrument with a remote connection to the system Shut down in an external instrument with a remote connection to the system The degasser failed to generate sufficient vacuum for solvent degassing Suggested Actions Fix the leak in the external instrument before restarting the nano pump Check external instrument...

Page 41: ...lysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable Causes Not ready condition in one of the instruments connected to the remote line Defective remote cable Defective components in the instrument showing the not ready condition Suggested Actions Ensure the instrument showing the not ready condition is installed correctly and i...

Page 42: ...the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable Causes CAN cable disconnected Defective CAN cable Defective main board in another module Suggested Actions Ensure all the CAN cables are connected correctly Switch off the system Restart the system and determine which module or modules are not reco...

Page 43: ...eak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the CSM board Probable Causes Loose fittings Broken capillary Loose or leaking active inlet valve outlet ball valve or EMPV Defective pump seals Suggested Actions Ensure all fittings are tight Exchange defective capillaries Ensure pump components are seated correctly If ...

Page 44: ... is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable Causes Leak sensor not connected to the CSM board Defective leak sensor Leak sensor incorrectly routed being pinched by a metal component Suggested Actions Ensure the leak sensor is connected correctly E...

Page 45: ...ak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable Causes Defective leak sensor Leak sensor incorrectly routed being pinched by a metal component Suggested Actions Exchange the leak sensor ...

Page 46: ...uit The resistance across the temperature compensation sensor NTC on the CSM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable Causes Defective CSM board Suggested Actions Exchange the CSM board ...

Page 47: ...ircuit The resistance across the temperature compensation sensor NTC on the CSM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable Causes Defective CSM board Suggested Actions Exchange the CSM board ...

Page 48: ...onitor the fan speed If the fan speed falls below 2 revolutions second for longer than 5 seconds the error message is generated Probable Causes Fan cable disconnected Defective fan Defective CSM board Improperly positioned cables or wires obstructing fan blades Suggested Actions Ensure the fan is connected correctly Exchange fan Exchange the CSM board Ensure the fan is not mechanically blocked ...

Page 49: ...place If the foam is removed the fan is switched off and the error message is generated Probable Causes The top foam was removed during operation Foam not activating the sensor Sensor defective Rear of the module is exposed to strong direct sunlight Suggested Actions Replace the top foam Exchange the CSM board Ensure that the rear of the module is not exposed to strong sunlight ...

Page 50: ...n the top foam is in place If the nano pump is restarted with the foam removed the nano pump switches off within 30 s and the error message is generated Probable Causes The nano pump started with the top cover and foam removed Rear of the module is exposed to strong direct sunlight Suggested Actions Replace the top cover and foam Ensure that the rear of the module is not exposed to strong sunlight...

Page 51: ...set solvent bottle fillings at the ChemStation revision 5 xx and higher If the volume level in the bottle falls below the specified value the error message appears when the feature is configured accordingly Probable Causes Volume in bottle below specified volume Incorrect setting of limit Suggested Actions Refill bottles and reset solvent counters ...

Page 52: ...ceeded the upper pressure limit Probable Causes Upper pressure limit set too low Blockage in the flowpath after the damper Defective damper Defective CSM board Suggested Actions Ensure the upper pressure limit is set to a value suitable for the analysis Check for blockage in the flowpath Exchange the damper Exchange the CSM board ...

Page 53: ...Leak Defective damper Defective CSM board Suggested Actions Ensure the lower pressure limit is set to a value suitable for the analysis Ensure solvents are degassed Purge the nano pump Ensure solvent inlet filters are not blocked Inspect the pump head capillaries and fittings for signs of a leak Purge the nano pump Run a pressure test to determine whether the seals or other pump components are def...

Page 54: ...ing The pressure signal from the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120mV across the damper connector Probable Causes Damper disconnected Defective damper Suggested Actions Ensure the damper is connected correctly to the CSM board Exchange the damper ...

Page 55: ...ve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable Causes Solvent selection valve disconnected Connection cable inside instrument not connected Connection cable inside instrument defective Solvent selection valve defective Suggested Actions Ensure the solvent selection valve is connected correctly Ensure the connecti...

Page 56: ...converter are missing The ADC reads the pressure readings from the damper every 1ms If the readings are missing for longer than 10 seconds the error message is generated Probable Causes Damper not connected Defective damper Defective CSM board Suggested Actions Ensure the damper connector is clean and seated correctly Exchange the damper Exchange the CSM board ...

Page 57: ...f the active inlet valve and pump encoder of channel B are disconnected and the nano pump is rebooted the error message is generated However the nano pump will function as an isocratic pump in this configuration The error message reappears after each switch on Probable Causes Active inlet valve and pump encoder of channel B disconnected Suggested Actions Reconnect the active inlet valve and pump e...

Page 58: ...es in the nano pump has drawn excessive current causing the selection valve electronic fuse to open Probable Causes Defective solvent selection valve Defective connection cable front panel to CSM board Defective CSM board Suggested Actions Restart the nano pump If the error message appears again exchange the solvent selection valve Exchange the connection cable Exchange the CSM board ...

Page 59: ...inlet valves in the nano pump has drawn excessive current causing the inlet valve electronic fuse to open Probable Causes Defective active inlet valve Defective connection cable front panel to CSM board Defective CSM board Suggested Actions Restart the nano pump If the error message appears again exchange the active inlet valve Exchange the connection cable Exchange the CSM board ...

Page 60: ...erature Out of Range 1 Pump channel B One of the temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable Causes Defective CSM board Suggested Actions Exchange the CSM board ...

Page 61: ... the circuits increase If the temperature exceeds the upper limit of 95 ºC the error message is generated Probable Causes High friction partial mechanical blockage in the pump drive assembly Partial blockage of the flowpath in front of the damper Defective drive assembly Defective CSM board Suggested Actions Ensure the capillaries and frits between the pump head and damper inlet are free from bloc...

Page 62: ...r the nano pump will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the nano pump is switched off and the error message is generated Probable Causes Flow path blockage in front of the damper Blocked outlet ball valve High friction partial mechanical blockage in the drive assembly Defective drive assembly Defective CSPM board Sugg...

Page 63: ...n the nano pump is missing or defective The processor checks the presence of the pump encoder connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable Causes Defective or disconnected pump encoder connector Defective pump drive assembly Suggested Actions Ensure the connector is clean and seated correctly Exchange the pump drive assembly ...

Page 64: ...e connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable Causes Disconnected or defective cable Disconnected or defective connection cable front panel to CSPM board Defective active inlet valve Suggested Actions Ensure the pins of the active inlet valve connector are not damaged Ensure the connector is seated securely Ensure the connecti...

Page 65: ...Electro Magnetic Proportional Valve EMPV Missing EMPV Missing The EMPV in the micro pump is missing or defective Probable Causes Disconnected or defective cable Defective solenoid Suggested Actions Ensure the connection cable is seated correctly Exchange the solenoid of the EMPV ...

Page 66: ... Pump Service Manual 2 Troubleshooting and Test Functions Flow sensor missing Probable Causes Flow sensor disconnected Defective flow sensor Suggested Actions Ensure the sensor is seated correctly Exchange the flow sensor ...

Page 67: ...o Pump Service Manual 55 Troubleshooting and Test Functions 2 Unsupported Flow Sensor Probable Causes A 20 µl min or a 100 µl min flow sensor is used with the nano pump Suggested Actions Change to the nano flow sensor ...

Page 68: ...vice Manual 2 Troubleshooting and Test Functions Leak Sensor Missing Probable Causes Disconnected or defective cable Defective leak sensor Suggested Actions Ensure the connection cable is seated correctly Exchange the leak sensor ...

Page 69: ... required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable Causes Disconnected or defective cables Mechanical blockage of the nano pump Defective pump drive assembly Defective CSM board Suggested Actions Ensure the pump assembly cables are not damaged or...

Page 70: ...es forward to the mechanical end stop Normally the end stop is reached within 20 seconds indicated by an increase in motor current If the end point is not found within 20 seconds the error message is generated Probable Causes Pump head not installed correctly screws not secured or pump head not seated correctly Broken plunger Suggested Actions Install the pump head correctly Ensure nothing e g cap...

Page 71: ...to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable Causes Irregular or sticking drive movement Defective pump drive assembly Suggested Actions Remove the pump head and examine the seals plungers and internal components for signs of wear...

Page 72: ...nical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the time to reach the index position is too long the error message is generated Probable Causes Irregular or sticking drive movement Defective pump drive assembly Suggested Actions Remove the pump head and examine the seals plungers and internal components for signs of wear c...

Page 73: ...ved to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable Causes Disconnected or defective encoder cable Defective pump drive assembly Suggested Actions Ensure the encoder cable are not damaged or dirty Make sure the ca...

Page 74: ...op is out of limits nano pump During initialization the nano pump monitors the drive current If the plunger reaches the upper mechanical stop position before expected the motor current increases as the nano pump attempts to drive the plunger beyond the mechanical stop This current increase causes the error message to be generated Probable Causes Defective pump drive assembly Suggested Actions Exch...

Page 75: ...lize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable Causes Blocked active inlet valve Defective pump drive assembly Defective CSM board Suggested Actions Exchange the active inlet valve Exchange the pump drive assembly Exchange the CSM...

Page 76: ... B did not reach the delivery phase Pump channel A did not reach the take in phase Pump channel B did not reach the take in phase Solvent volume not delivered within the specified time Probable Causes System still in purge mode Leak at fittings EMPV active inlet valve outlet ball valve or plunger seals Flow changed after starting test Defective pump drive assembly Suggested Actions Ensure that pur...

Page 77: ...e pump is operating in the pressure control mode at 380 bar for several minutes The remaining flow in the column flow path between the EMPV and the blank nut is measured Running the Test 1 Select the ChemStation s Diagnosis screen from the nano pump tests selection box select Micro Mode Pressure Test 2 Start the test and follow the online instructions NOTE Make absolutely sure that all parts of th...

Page 78: ...ions Micro Mode Pressure Test Results The test results are evaluated automatically The sum of all leaks within the column flow path from the EMPV to the blank nut must be lower than100 nl min NOTE Small leaks with no visible leaks in the flow path can cause the test to fail ...

Page 79: ...t the pressure test to confirm the system is tight Potential Cause Pump Corrective Action Loose or leaky fitting Tighten the fitting or exchange the capillary Untight EMPV Run the EMPV test Damaged pump seals or plungers Run the leak test to confirm the leak High flow sensor offset Run the flow sensor accuracy calibration and correct the flow sensor offset Potential Cause Autosampler Corrective Ac...

Page 80: ...ce outlet port 6 is blocked with a blank nut and then the test is run using isopropyl alcohol IPA while monitoring the pressure profile using an integrator on the analog output or in the plot screen in the ChemStation The pressure profile is shown in Figure 8 Step 1 The test begins with the initialization of both pumpheads After initialization plungers A1 and B1 are both at the top of their stroke...

Page 81: ...ion box in the Diagnosis screen 2 Start the test and follow the instructions NOTE For this test only channel A2 is active To test the pressure tightness of the pump component use the leak test see Leak Test on page 73 Tools required Wrench 1 4 inch Parts and materials required Blank nut 01080 83202 Isopropanol 500 ml NOTE This test requires the manual purge valve installed and the Electronic Flow ...

Page 82: ...le 1 Place a bottle of LC grade isopropyl alcohol in the solvent cabinet and connect it to channel A2 2 Block the injection device port 6 with a blank nut 01080 83202 Positioning the blank nut on page 69 3 Set flow for channel A2 to 2500 µl min and flush the degasser channel for about 5 minutes 4 Set flow to 0 µl min 5 Connect the signal cable to the analog output at the rear of the nano pump only...

Page 83: ...sure all fittings between the pump and the blank nut are tight Repeat the pressure test If the test fails again insert the blank nut at the outlet of the previous module in the stack and repeat the pressure test Exclude each module one by one to determine which module is leaking If the pump is determined to be the source of the leak run the EMPV cleaning procedure repeat the pressure test and if t...

Page 84: ...tion Loose or leaky fitting Tighten the fitting or exchange the capillary Damaged pump seals or plungers Run the leak test to confirm the leak Potential Cause Autosampler Corrective Action Loose or leaky fitting Tighten or exchange the fitting or capillary Needle seat Exchange the needle seat Rotor seal injection valve Exchange the rotor seal Damaged metering seal or plunger Exchange the metering ...

Page 85: ...alcohol IPA while monitoring the pressure profile using an integrator on the analog output or in the plot screen in the Control Module or the ChemStation The pressure profile is shown in Figure 9 Ramp 1 The test begins with the initialization of both pumps After initialization plungers A1 and B1 are both at the top of their stroke Next the nano pump begins pumping solvent with a flow rate of 150 µ...

Page 86: ...es a leak rate greater than 3 µl min Ramp 4 Plunger B2 delivers 50 µl min for approximately 8 seconds Plateau 2 Plunger B2 delivers with a flow rate of 3 µl min for 30 seconds The slope should be horizontal or slightly positive A negative slope indicates a leak rate greater than 3 µl min Ramp 5 Plunger A1 delivers 50 µl min for approximately 8 seconds Plateau 3 Plunger A1 with a flow rate of 3 µl ...

Page 87: ...he Diagnosis screen 2 Start the test and follow the instructions NOTE Make absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with IPA before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail NOTE This test requires the manual purge valve installed a...

Page 88: ...ry with blank nut 01080 83202 4 Connect the signal cable to the analog output at the rear of the nano pump only if an integrator is used 5 Press Execute to initialize the leak test Once the test is started the nano pump increase the pressure and run each plunger at low flow rate The control module displays a graphical representation of the pressure in the plateau windows Evaluating the Results of ...

Page 89: ...r in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test where not within the specified limits NOTE Often it is only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure...

Page 90: ... all fittings are tight or exchange capillary Large leaks visible at the pump seals Exchange the pump seals Large leaks visible at active inlet valve outlet valve Ensure the leaky components are installed tightly Exchange the component if required Pressure limit not reached but plateaus horizontal or positive Potential Cause Corrective Action Degasser and pump channels A and or B not flushed suffi...

Page 91: ...ary Leaky mixer if installed Tighten the mixer fittings and nuts Loose pump head screws in channel A or B Ensure the pump head screws in channels A and B are tight Leaking seal or scratched plunger in channel A2 or B2 Exchange the pump seals in both channels Check the plungers for scratches Exchange if scratched Leaking outlet valve in channel A or B Exchange the outlet valve Leaky damper Exchange...

Page 92: ...on Leaking outlet valve in channel A Clean the outlet valve in channel A Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Loose pump head screws in channel A Ensure the pump head screws in channel A are tight Leaking seal or scratched plunger in channel A2 Exchange the pump seals in channel A Check the plunger for scratches Exchange if scratched ...

Page 93: ...on Leaking outlet valve in channel B Clean the outlet valve in channel B Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Loose pump head screws in channel B Ensure the pump head screws in channel B are tight Leaking seal or scratched plunger in channel B2 Exchange the pump seals in channel B Check the plunger for scratches Exchange if scratched ...

Page 94: ...inlet valve in channel A Tighten the active inlet valve in channel A 14mm wrench Do not overtighten Loose pump head screws in channel A Ensure the pump head screws in channel A are tight Loose outlet valve in channel A Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve Leaking seal or scratched plunger in channel A1 Exchange the pump seals in channel A Check the p...

Page 95: ...tive inlet valve in channel B Tighten the active inlet valve in channel B 14mm wrench Do not overtighten Loose pump head screws in channel B Ensure the pump head screws in channel B are tight Loose outlet valve in channel B Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve Leaking seal or scratched plunger in channel B1 Exchange the pump seals in channel B Check ...

Page 96: ...est or pressure test The leak and pressure tests should also be done when leaks within the pump heads might be the problem Step 1 The test starts with a short flushing sequence and a cleaning procedure for the EMPV Step 2 Afterwards low and high pressure is controlled by the EMPV and the appropriate current is monitored Step 3 Finally a linear pressure ramp is performed Running the test 1 Select t...

Page 97: ...tomatically Two pressure plateaus are checked one at 20 bar and the other at 300 bar The appropriate current is measured A further criterion is the difference between the two plateaus The limits are as follows 85 EMPV current for the 20 bar plateau 15 EMPV current for the 300 bar plateau 15 difference between both EMPV current values ...

Page 98: ...The pump is operating in the flow control mode and is delivering 2 µl min for approximately 3 minutes At the end of this step the pump is switched to pressure control mode The actual pressure is the initial pressure Step 4 The pump is delivering solvent for 3 minutes at a pressure equal to the initial pressure divided by 2 At the end of this step the corresponding flow sensor response is measured ...

Page 99: ...ests selection box select Flow Sensor Accuracy Calibration Test 7 Start the test and follow the online instructions NOTE Make absolutely sure that all parts of the flow path that are included in the test are very thoroughly flushed with WATER before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path will definitely cause the test to fail N...

Page 100: ... values are evaluated automatically A table with the measured pressure drop over the inlet filter the measured flow and pressure offset and the correction factors for all steps are printed You have now the possibility to accept and store all the correction factors and the measured offset accept only the measured offset reset no correction factor or offset are applied ...

Page 101: ...aluation Pressure drop over filter The measured value should not be higher than 10 Bar If higher we recommend to change the filter in front of the EMPV and restart the test again Offset flow This value can be positive or negative and should be between 500 nL min and 90 nL min If higher or lower replace the nano flow sensor Offset pressure sensor This value has not to be considered in the evaluatio...

Page 102: ...he test If value still out of range accept the offset only at the end of the test If the flow sensor has a negative offset the minimum flow rate the pump can operate is the numeric value of the measured offset For example If offset flow is 0 030 µL min the minimum flow rate the pump can operate is 30 nL min The offset of the nano flow sensor has a more significant influence on the flow sensor accu...

Page 103: ... of being leaky or contaminated with particles Step 1 The outlet of the EMPV is plugged with a blank nut After a short flushing routine the EMPV is closed and the pressure is increased to approximately 380 bar Step 2 The EMPV is then opened and the pressure is released very quickly Step 3 This procedure is repeated several times in a sequence Running the Cleaning procedure 1 Select the ChemStation...

Page 104: ...mally High 94 EMPV failed to initialize micro mode only 95 Unstable column flow and or system pressure 96 Poor peak shape 98 Failure to produce peaks or abnormally small peaks after injection 99 Wandering Detector Baseline 100 User interface displays error messages for specific modules 101 NOTE This troubleshooting guide deals with systemic problems of the capillary LC For detailed diagnostic trou...

Page 105: ...roughout the system At very low flow rates a leak may never accumulate enough liquid to trigger the module leak sensors Low flow rate leaks are also very hard to see Solvent channels are not correctly purged Ripple might also be too high Perform a 4 minute purge at 2500 µl min for each solvent channel This is especially likely if the system has been unused for more than one day Dirty solvent inlet...

Page 106: ...bly or injection valve ports Using the sampler maintenance positions switch the sampler injection valve from mainpass to bypass If pressure is significantly reduced Backflush or change the needle seat assembly Replace the needle Backflush or replace the sample loop capillary Replace the injection valve rotor seal Clean the stator head with acetone and make sure the stator head ports are free of pa...

Page 107: ...al This problem typically causes a permanent EMPV Initialization not ready message The inlet to the EMPV has been blocked or partially restricted The EMPV cannot take in sufficient flow to deliver the correct flow output The EMPV initialization routine cannot be done within the required 2 minute period Make sure the solvent channels are well purged Check the EMPV filter Perform a pump purge at 100...

Page 108: ...iable flow control micro mode Make sure that there is at least 20 bar pressure being developed after the pump Add an additional capillary after the pump if required Leaks somewhere in the system Use a flashlight and absorbent tissue to search for leaks throughout the system Check for leaks after the pump and inside the pump valves fittings etc If operating in the micro mode perform the micro mode ...

Page 109: ... restriction to the pump Replace the analytical column Replace the filter frit in front upstream of the EMPV The vacuum micro degasser is off or has become defective Try another vacuum micro degasser or experiment to determine the performance using different degasser channels If the mobile phase is very sensitive to gaseousness use the micro degasser continuous mode Basic performance problems in t...

Page 110: ...apter 3 for information on connecting capillaries Make sure the capillary connections are correctly made throughout the system especially in the following areas a All micro sampler valve ports b Column inlet and outlet c Flow cell inlet capillary at the capillary cell body junction At very low flow rates a leak may never accumulate enough liquid to trigger the module leak sensors Low flow rate lea...

Page 111: ... very low flow rates a leak may never accumulate enough liquid to trigger the module leak sensors Low flow rate leaks are also very hard to see The 40 µl chamber of the micro samplermeteringhead has developed bubbles In the user interface diagnostics access the Change Piston function of the micro sampler maintenance positions This function draws the metering piston fully inward clearing the chambe...

Page 112: ... Noise when pumping a binary mobile phase from two channels Try premixing the mobile phase in one bottle and pumping 100 from that one solvent channel If baseline performance improves a solution must be found which is a compromise between mixing volume and other chromatographic requirements For an isocratic analysis premixing and pumping 100 one channel is the best solution This problem occurs whe...

Page 113: ...ecific modules Possible causes and suggested actions Possible Causes Suggested Actions Notes A module has experienced a specific hardware failure during operation Refer to the Reference Manual supplied with the module Follow the advice on troubleshooting and repair for the error message displayed A specific error message for that module is displayed The status indicator of that module is red ...

Page 114: ...102 1100 Series Nano Pump Service Manual 2 Troubleshooting and Test Functions ...

Page 115: ...xchanging the Purge Valve Frit or the Complete Manual Purge Valve 115 Exchanging the EMPV Assembly 117 Exchanging the Solvent Selection Valve 118 Removing and Disassembling the Pump Head Assembly 120 Exchanging the Pump Seals and Seal Wear in Procedure 122 Exchanging the Plungers 125 Exchanging the Flow Sensor 126 Reassembling the Pump Head Assembly 127 Exchanging the Optional Interface Board 129 ...

Page 116: ...eries Nano Pump Service Manual 3 Repairing the Pump Exchanging Status Light Pipe 152 Assembling the Main Cover 153 Replacing the Top Cover and Foam 154 This chapter includes Instruction on how to repair the pump ...

Page 117: ... the pump cover is taken off when line power is still connected WARNING When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used WARNING To prevent ...

Page 118: ...er to prevent damage always use an ESD strap supplied in the standard accessory kit see Nano Pump Accessory Kit G2226 68705 on page 172 when handling electronic boards and components Using the ESD Strap 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Atta...

Page 119: ... exchange and can be accessed from the front simple repairs Replacing internal parts will require removing the module from its stack and to open the top cover Figure 13 Overview of Repair Procedures 1 Pumpdrive A 8 Main Board 2 Damper 9 Power Supply 3 EMPV 10 Fan 4 Outlet Ball Valve A 11 Flow Sensor 5 Pumphead A 12 Pumphead B 6 Solvent Selection Valve 13 Active Inlet Valve B 7 Leak Sensor 8 9 10 1...

Page 120: ...lling the Manual Purge Valve on page 114 Unstable column flow or system pressure Installing the Manual Purge Valve on page 114 Column flow and system pressure drops from time to time A pressure drop of 10 bar across the frit 2 5 ml min H2O with purge open indicates blockage Exchanging the Pump Seals and Seal Wear in Procedure on page 122 If pump performance indicates seal wear Leaks at lower pump ...

Page 121: ...tube at the inlet valve Beware of leaking solvents 3 Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head Exchanging the Valve Cartridge 1 Using a pair of tweezers remove the valve cartridge from the actuator assembly Frequency If internally leaking backflow Tools required Wrench 14 mm Parts required Active inlet valve G1312 60010 Valve Cartridge 5062 8562 Figure ...

Page 122: ...t 2 Position the valve that the solvent inlet tube connection points towards the front 3 Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn Do not overtighten the valve The solvent inlet tube connection should point to the right corner of the pump head 4 Reconnect the inlet tube and the active inlet valve cable to the connector at the Z p...

Page 123: ...1100 Series Nano Pump Service Manual 111 Repairing the Pump 3 Figure 15 Exchanging the Active Inlet Valve Active inlet valve Solvent inlet tube Plug ...

Page 124: ...s present Frequency Sieve whenever the pump seals will be exchanged Valve if internally leaking Tools required Wrench 1 4 inch Wrench 14 mm Parts required Outlet ball valve G1312 60008 Sieve pack of 10 5063 6505 NOTE Before exchanging the outlet ball valve you can try to clean it in a sonic bath Remove the gold seal and the sieve Place the valve in upright position onto the plastic cap in a small ...

Page 125: ...g the Pump 3 7 Reinstall the outlet ball valve and tighten the valve 8 Reconnect the valve capillary Figure 16 Outlet Ball Valve Parts Figure 17 Exchanging the Outlet Ball Valve Valve body Gold seal Plastic cap Sieve Valve capillary Outlet ball valve ...

Page 126: ...r 6 Insert the purge valve assembly into the purge valve holder and locate the outlet and waste outlet as shown below 7 Use the 14 mm wrench to tighten the purge valve assembly 8 Connect the waste tube from the EMPV assembly to the waste outlet of the purge valve 9 Connect the pump outlet capillary into the outlet of the purge valve Frequency When performing a leak test for verifying pump tightnes...

Page 127: ...n plunger seals are exchanged or when contaminated or blocked pressure drop of 10 bar across the frit at a flow rate of 2500 µl min of H20 with purge valve opened Purge valve if internally leaking Tools required Wrench 1 4 inch Wrench 4 mm depending on pump outlet capillary Wrench 14 mm Pair of tweezers or toothpick Parts required PTFE Frit pack of 5 01018 22707 Purge valve G1311 60009 Figure 18 E...

Page 128: ... frit into the purge valve with the orientation of the frit as shown above 7 Replace the cap with the gold seal 8 Insert the purge valve into the purge valve holder and locate the pump outlet and the waste outlet as shown below 9 Tighten the purge valve reconnect outlet capillary and waste tubing Figure 19 Purge Valve Parts Valve body Gold seal PTFE frit Plastic cap NOTE Before reinstallation alwa...

Page 129: ...olvents due to hydrostatic pressure 5 Disconnect the EMPV connector P9 on the main board see Figure 22 on page 135 6 Using a hex key unscrew the complete EMPV and remove it 7 Screw a new one in place 8 Reconnect the capillaries going to the flow sensor coming from the filter 9 Run the EMPV test see EMPV Test on page 84 Frequency If valve internally leaking If unstable column flow in micro mode If ...

Page 130: ...lvent tubes into the solvent cabinet to prevent leaks due to hydrostatic flow Frequency If internally leaking crossfoot between the ports or if one of the channels is blocked Tools required Screwdriver Pozidriv 1 Parts required Solvent selection valve PN gives half of a complete solvent selection block G1312 60000 Figure 20 Exchanging the solvent selection valve Connecting tubes Solvent selection ...

Page 131: ... the two plastic bodies of the valves and pull the two solvent selection valves apart 5 Exchange the defective solvent selection valve Press the exchanged valve new half together with the properly working old half 6 Connect the valve module to its electrical connectors and fix the assembly with the two holding screws 7 Reinstall solvent tubes and the active inlet valve connection tubes ...

Page 132: ...g the plungers Exchanging seals of the seal wash option Tools required Wrench 1 4 inch 3 mm hexagonal key 4 mm hexagonal key Preparations for this procedure Switch off nano pump at power switch Remove the front cover to have access to the pump mechanics 1 Disconnect the capillary at the pumphead adapter and the tube at the active inlet valve Beware of leaking solvents Disconnect the active inlet v...

Page 133: ...rom the pump drive 3 Place the pump head on a flat surface Loosen the lock screw two revolutions While holding the lower half of the assembly carefully pull the pump head away from the plunger housing 4 Remove the support rings from the plunger housing and lift the housing away from the plungers Pumphead screws Lock screw Pump head Support rings Plunger housing Plunger ...

Page 134: ...st Check both pump heads individually Tools required 3 mm hexagonal key 4 mm hexagonal key 1 4 inch wrench Parts required Seals pack of 2 5063 6589 standard or 0905 1420 for normal phase application For the seal wear in procedure Restriction capillary 5022 2159 1 Disassemble the pump head assembly of the leaky pump head see Removing and Disassembling the Pump Head Assembly on page 120 ...

Page 135: ...ee cloth Ensure all particulate matter is removed Best cleaning results will be achieved by removing all valves see pages 109 112 and the capillary Inject solvent into each chamber 4 Insert seals into the pump head and press firmly in position 5 Reassemble the pump head assembly see Reassembling the Pump Head Assembly on page 127 Reset the seal wear counter and liquimeter as described in the User ...

Page 136: ...in 5 Turn the system to standard mode set the flow to a rate adequate to achieve a pressure of 350 bar Pump 15 minutes at this pressure to wear in the seals The pressure can be monitored at your analog output signal with the handheld controller Chemstation or any other controlling device connected to your pump 6 Turn OFF the pump slowly disconnect the restriction capillary from the EMPV to release...

Page 137: ...Parts Plunger 5063 6586 1 Disassemble the pump head assembly see Removing and Disassembling the Pump Head Assembly on page 120 2 Check the plunger surface and remove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace plunger if scratched 3 Reassemble the pump head assembly see Reassembling the Pump Head Assembly on page 127 Plunger surface ...

Page 138: ... sensor accuracy calibration capillary G2226 67300 on the flow sensor outlet 8 Run the flow sensor accuracy calibration from the ChemStation Follow the step by step procedure 9 At the end of the procedure disconnect the flow sensor accuracy calibration capillary 10 Reconnect the flow sensor to sampler capillary on the flow sensor outlet Frequency Leak on the flow sensor Unstable column flow Flow s...

Page 139: ... PTFE lubricant 79841 65501 1 Place the support rings on the plunger housing plungers notinstalled andsnapthe pump head and plunger housing together 2 Tighten the lock screw 3 Carefully insert the plungers into the pump head assembly and press them completely into the seals Pump head Support ring plunger housing Lock screw Plunger Pump head assembly ...

Page 140: ...small amount of pump head grease to the pumphead screws and the balls of the spindle drive Tighten screws stepwise with increasing torque 5 Reconnectthe capillaries tubing and the active inlet valve cable to the connector Balls of spindle drive Pump head screws AIV connector Inlet tube Adapter capillary Active inlet valve ...

Page 141: ...osen the screws Slide out the interface board from the nano pump 4 Install the new interface board Secure screws 5 Reconnect the cables to the board connector CAUTION The interface board is sensitive to electrostatic discharge Always use the ESD kit when handling electronic boards When required Board defective Part required BCD Interface board see Optional Interface Boards on page 215 Figure 21 Ex...

Page 142: ...er supply still uses some power even if the switch on the front panel is turned off WARNING When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used...

Page 143: ...ect the solvent inlet tubes from the solvent selection valve or the adapter at the active inlet valve Beware of leaking solvents due to hydrostatic flow Remove leak funnel with the waste tube Remove the solvent cabinet from the nano pump 1 Remove the front cover by pressing the clip fastener on both sides of the cover 2 Unplug the power cord and move the lever towards the power socket Clip fastene...

Page 144: ...e top cover 1 Remove the top cover 2 4 Unscrew the screws on the top plate and remove the plate by lifting its back first and then sliding to the front 5 If an optional interface board is installed remove it from the nano pump 6 Remove the top foam Cover Cover clip 1 1 2 2 Screws Safety light switch ...

Page 145: ... the nano pump module after removing the top covers A safety light switch on the main board will prevent operation when the covers are removed The next figure shows the position of the light switch on the board 7 Position of the safety switch on the main board Light switch Top foam Foam lever ...

Page 146: ...ive Tools required Wrench 14 mm Wrench 7 mm Wrench 5 mm Parts required NPM board G2226 66530 exchange part number G2226 69530 Connector Description Connector Description S1 Configuration switch J1 GPIB P11 AIV A J2 CAN connector P15 Leak sensor J3 CAN connector P16 Damper J4 RS232 P17 Fan J5 Remote P18 Interface board J6 Analog output P21 Encoder B P2 Power supply P20 AIV B P8 Motor B P22 Solvent ...

Page 147: ... ambient remains on the interface connectors of the board Carefully remove the spring plate from the old board and slide onto the new board before installing the new board in the pump the RFI spring plate is NOT part of an exchange board Figure 22 Board Layout of the NPM Board S1 J1 J2 J3 J4 J6 P2 P8 P20 P22 P21 P16 P17 P15 P18 P9 P11 J5 P19 P14 P12 WARNING The RFI spring plate sitting on the boar...

Page 148: ...e Replacing the Top Cover and Foam on page 154 12 Reinstall the pump in the stack Reconnect all cables Turn on the nano pump If the status indicator turns red continue with the section Entering NOTE An incorrect switch setting e g TEST BOOT may cause the pump to turn into a basic mode yellow or red flashing status light In such a case turn off the pump re set the address switches and turn on the p...

Page 149: ...at the nano pump is highlighted and press Enter The Tests screen for the nano pump should now be displayed 4 While in the Tests screen press m m m dot m From the box now displayed select the Command line and press Enter 5 Into the box labeled Instr enter the command TYPE G2226A NOTE After the installation of a new mainboard the TYPE binary of the module is normally automatically detected The speci...

Page 150: ...nt sendmodule lpmp TYPE G2226A 2 The reply line will respond with RA 0000 Type G2226A 3 Turn the nano pump off then on again Then re boot the ChemStation Boot up and subsequent control of the system should be normal 4 The TYPE of a module can also be identified by typing the following command into the command line print sendmodule lpmp TYPE The reply line will give the module TYPE NOTE Letters and...

Page 151: ... to highlight the complete serial number Then press Done F6 6 Turn the nano pump off then on again The Records screen should display the correct serial number 7 If a ChemStation is also connected re boot the ChemStation now as well Entering the Serial Number using the ChemStation Module serial numbers are entered by typing specific commands into the command line at the bottom of the main user inte...

Page 152: ...e screen The serial number of the nano pump can also be seen by typing the following command into the command line print sendmodule lpmp ser The reply line will give the serial number of the nano pump Replacing the Nano Pump s Firmware The installation of new firmware is required if a new version solves problems of the currently installed version if the version of firmware on the new main board NP...

Page 153: ...ion device port 1 5 Unclip the mixing chamber from its holder 6 Remove the damper and the Z panel For this disconnect first the restriction capillary the capillary going to the mixer the connector P16 P11 P20 at the main board see Figure 22 on page 135 7 Unclip the mixing chamber from its holder 8 Loosen the screws of the Z panel fold it forward and remove the damper 9 Re install the Z panel and t...

Page 154: ...install the flow sensor Reconnect the capillaries and the cable 12 Re install the EMPV Reconnect the capillaries the waste tube and the cable 13 Reconnect both active inlet valve cables 14 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 154 ...

Page 155: ... mixing chamber from its holder 5 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 135 6 Remove pump assembly B For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solven...

Page 156: ...mbly B Reconnect all capillaries and connectors P8 P21 10 Re install the Z panel and the damper Reconnect the capillaries and connectors P16 P11 P20 11 Re install the mixing chamber to its holder 12 Re install the flow sensor Reconnect the capillaries and the cable 13 Re install the EMPV Reconnect the capillaries the waste tube and the cable 14 Replace the top foam section optional interface board...

Page 157: ...he flow sensor For this disconnect first the capillary coming from the EMPV the capillary on the injection device port 1 4 Remove the pump head For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solvent selection valve the active inlet valve cable at the Z panel 5 Unclip the mixing chamber from its holder 6 Remove the damper and the Z panel F...

Page 158: ...drive into the recess Reconnect the connectors at the main board 9 Re install the Z panel and the damper Reconnect the capillaries and connectors P16 P11 P20 10 Re install the mixing chamber to its holder 11 Re install the pump head Reconnect the capillaries and the cable 12 Re install the flow sensor Reconnect the capillaries and the cable 13 Re install the EMPV Reconnect the capillaries the wast...

Page 159: ...the injection device port 1 the connector 4 Unclip the mixing chamber from its holder 5 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 135 6 Remove both pump assemblies For this disconnect first the capillary coming from th...

Page 160: ...the two screws at the rear panel 13 Place the coupler onto the switch in the power supply and clip the light pipe back onto the coupler 14 Slide the leak sensor cable through the bottom foam and fix the leak sensor cable in the foam Make sure the leak sensor cable is not damaged by the sheet metal 15 Re install the CSM board Reconnect all the cables 16 Re install the solvent selection valve 17 Re ...

Page 161: ...device port 1 the connector 4 Unclip the mixing chamber from its holder 5 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 135 6 Remove both pump assemblies For this disconnect first the capillary coming from the mixing chamb...

Page 162: ...ee Figure 24 and replace the pan Make sure that the cable is located in the recess of the bottom metal plate 14 Reinstall the bottom foam Make sure that the leak senor cable is guided through the foam to the top side 15 Replace the main board see Exchanging the Nano Pump Main Board NPM Board on page 134 16 Re install the solvent selection valve 17 Re install the two pump head Reconnect the capilla...

Page 163: ...1100 Series Nano Pump Service Manual 151 Repairing the Pump 3 Figure 24 Exchanging the Leak Sensor Leak sensor Leak pan To main board J23 ...

Page 164: ...Status light pipe 5041 8384 Preparation for this procedure Remove the front cover and top cover see Removing the Top Cover and Foam on page 131 1 The status light pipe is clipped into the top cover 2 Replace the top cover see Replacing the Top Cover and Foam on page 154 3 Replace the nano pump into the stack and reconnect the cables and capillaries 4 Turn on the nano pump ...

Page 165: ...OTE The cover kit contains all parts but it is not assembled WARNING In case you insert the left or right side in the opposite position you may not be able to remove the side from the top part 1 Place the top part on the bench and insert the left and right side into the top part 2 Replace the cover 3 Replace the nano pump into the stack and reconnect the cables and capillaries 4 Turn on the nano p...

Page 166: ...es cables capillaries and connectors are located in its correct place 1 Place the damper cable in a curve to the right side towards the active inlet valve cable of pump B Replace the top foam section 2 Make sure that the foam is installed correctly and is located in the safety light switch 3 Location of foam in the light switch 4 Replace the optional interface board or the board cover plate Light ...

Page 167: ... in the front then lower the back of the metal plate 2 and fix the two holding screws 6 Replace the top cover 7 Ensure clips are seated correctly move lever back 8 Replace the leak funnel with the waste tube Locate the lower end of the waste tube in the holder of the leak pan Holding screws 2 1 Clip Lever Waste funnel Leak pan Waste tube holder ...

Page 168: ... the nano pump back to its position in the stack replace solvent cabinet and reconnect all tubes and capillaries figure shows configuration with solvent selection valve 10 Replace the front covers and reconnect the power cable to the nano pump 11 Switch on the nano pump ...

Page 169: ...embly 163 Electro Magnetic Proportional Valve EMPV 165 Flow Sensor Assembly 166 Power and Status Light Pipes 167 Leak Parts 168 Cover Parts 169 Sheet Metal Kit 170 Foam Parts 171 Nano Pump Accessory Kit G2226 68705 172 Control Module G1323B 173 Cable Overview 174 Analog Cables 176 Remote Cables 179 BCD Cables 184 Auxiliary Cable 187 CAN Cable 188 External Contact Cable 189 RS 232 Cable Kit 190 LAN...

Page 170: ...158 1100 Series Nano Pump Service Manual 4 Parts and Materials Nano Pump Main Assemblies Figure 25 Nano pump main assembly 1 2 3 4 5 6 7 8 9 10 11 12 13 ...

Page 171: ...ble G1312 61602 4 Flow sensor G1376 60004 5 Leak pan pump 5041 8390 6 Solvent selection valve half of a complete valve G1312 60000 Solvent selection valve screw 5022 2112 7 Pump drive assembly Exchange pump drive assembly G1311 60001 G1311 69001 8 Pump head see page 163 G1311 60004 9 EMPV holding screw 0515 0850 10 EMPV complete assembly valve and solenoid G1361 60000 11 AIV connecting cable G1311...

Page 172: ...063 6599 3 Ferrules with lock ring pack of 10 5063 6598 4 Bottle amber Bottle transparent 9301 1450 9301 1420 5 Solvent inlet filter SST 01018 60025 6 Leak pan solvent cabinet 5042 1307 7 Front panel solvent cabinet 5062 8580 8 Name plate Agilent 1100 5042 1381 Bottle head assembly includes items 1 2 3 5 The assy comes with a glass solvent inlet filter Remove and replace it with the SST solvent in...

Page 173: ... to inj valve cap 350 mm 25 µm for nanoflow sensor FS to inj valve cap 550 mm 25 µm for nanoflow sensor G1375 87322 G1375 87323 4 Mixing capillary G1312 67302 5 Connection tube G1311 67304 6 Restriction capillary G1312 67304 7 Filter assembly includes frit Frit 5064 8273 5022 2185 8 Filter to EMPV cap 280 mm 170 µm G1375 87400 9 Damper to filter capillary 130 mm 250 µm 01090 87308 10 Solvent tube ...

Page 174: ...162 1100 Series Nano Pump Service Manual 4 Parts and Materials Figure 27 Nano pump hydraulic path 1 9 3 6 5 8 7 10 4 2 ...

Page 175: ... 5042 1303 3 Screw M5 60 mm 0515 2118 4 Apdater G1312 23201 5 Pump chamber housing G1311 25200 6 Active inlet valve complete with cartridge Replacement cartridge for active inlet valve G1312 60010 5062 8562 7 Seal pack of 2 Seal pack of 2 for normal phase applications 5063 6589 0905 1420 8 Plunger housing including springs G1311 60002 9 Sapphire plunger 5063 6586 10 Support ring 5001 3739 11 Outle...

Page 176: ...164 1100 Series Nano Pump Service Manual 4 Parts and Materials Figure 28 Pump Head Assembly 1 2 3 5 6 7 11 9 4 8 10 ...

Page 177: ... Magnetic Proportional Valve EMPV Table 19 EMPV Assembly Item Description Part Number 1 EMPV complete assembly G1361 60000 2 Filter to EMPV capillary G1375 87400 3 EMPV to flow sensor capillary nanoflow sensor G1375 87321 4 Screw 0515 0850 Figure 29 EMPV Assembly 1 2 3 4 ...

Page 178: ...nsor Assembly Item Description Part Number 1 Nanoflow sensor assembly G1376 60004 EMPV to flow sensor capillary nanoflow sensor G1375 87321 FS to inj valve cap 350 mm 25 µm for nanoflow sensor FS to inj valve cap 550 mm 25 µm for nanoflow sensor G1375 87322 G1375 87323 Figure 30 Flow Sensor Assembly 1 ...

Page 179: ...nd Status Light Pipes Table 21 Power and Status Light Pipes Item Description Part Number 1 Light pipe power switch 5041 8382 2 Power switch coupler 5041 8383 3 Light pipe status lamp 5041 8384 4 Power switch button 5041 8381 Figure 31 Power and Status Light Pipes 1 2 3 4 ...

Page 180: ...k Parts Table 22 Leak Parts Item Description Part Number 1 Holder leak funnel 5041 8389 2 Leak funnel 5041 8388 3 Tube clip 5041 8387 4 Leak plane pump 5041 8390 5 Leak sensor 5061 3356 6 Corrugated waste tube reorder pack 5m 5062 2463 Figure 32 Leak Parts 1 2 3 5 4 6 ...

Page 181: ...9 Parts and Materials 4 Cover Parts Table 23 Cover Parts Item Description Part Number 1 Plastic cover kit includes top base and both sides G1312 68703 2 Front plate G1376 60011 3 Logo plate Agilent 1100 5042 1381 Figure 33 Cover Parts 3 1 2 ...

Page 182: ...ials Sheet Metal Kit Table 24 Sheet Metal Kit Item Description Part Number 1 Sheet metal kit includes top base and Z panel G1376 68701 2 Included in item 1 Screw for cover and Z Panel 5022 2112 3 Board cover 5001 3772 Figure 34 Sheet Metal Kit Z panel 3 2 2 1 ...

Page 183: ... and Materials 4 Foam Parts Table 25 Foam Parts Item Description Part Number 1 Foam kit including upper and lower foam G1312 68702 2 Bushing for pump drive 1520 0404 3 Board guide 5041 8395 Figure 35 Foam Parts 1 upper foam 3 2 1 lower foam 3 2 2 2 ...

Page 184: ... x1 8710 0510 Wrench open end 14 mm x 1 8710 1924 Wrench open end 4 mm x 1 8710 1534 Hex key 2 5 mm 15 cm long straight handle x 1 8710 2412 Hex key 3 0 mm 12 cm long x 1 8710 2411 Hex key 4 0 mm 15 cm long T handle x 1 8710 2392 Torque adapter G1315 45003 CAN cable 1 m long 5181 1519 Purge valve assembly G1311 60009 Purge valve holder G1312 23200 Screw for the purge valve holder 0515 0175 FS to I...

Page 185: ...ule G1323B Table 27 Control Module Parts Item Description Part Number Control Module replacement part including cable G1323 67001 Plastic Housing Kit includes front back and a clamp 5062 8583 CAN cable Agilent 1100 module to control module G1323 81600 Figure 36 Control Module ...

Page 186: ...rter 35900 60750 General purpose spade lugs 01046 60105 Remote cables 3390 integrator 01046 60203 3392 3 integrators 01046 60206 3394 integrator 01046 60210 3396A Series I integrator 03394 60600 3396 Series II 3395A integrator see page 181 3396 Series III 3395B integrator 03396 61010 HP 1050 modules HP 1046A FLD 5061 3378 HP 1046A FLD 5061 3378 Agilent 35900A A D converter 5061 3378 HP 1040 diode ...

Page 187: ...1323 81600 External contacts Agilent 1100 Series interface board to general purpose G1103 61611 GPIB cable Agilent 1100 module to ChemStation 1 m 10833A Agilent 1100 module to ChemStation 2 m 10833B RS 232 cable Agilent 1100 module to a computer This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter 34398A LAN cable Twisted pair cross over LAN cable 10 feet long ...

Page 188: ... to Agilent 1100 Series modules The other end depends on the instrument to which connection is being made Table 29 Agilent 1100 to 3390 2 3 Integrators Connector 01040 60101 Pin 3390 2 3 Pin Agilent 1100 Signal Name 1 Shield Ground 2 Not connected 3 Center Signal 4 Connected to pin 6 5 Shield Analog 6 Connected to pin 4 7 Key 8 Not connected ...

Page 189: ...ent 1100 to 3394 6 Integrators Table 31 Agilent 1100 to BNC Connector Connector 35900 60750 Pin 3394 6 Pin Agilent 1100 Signal Name 1 Not connected 2 Shield Analog 3 Center Analog Connector 8120 1840 Pin BNC Pin Agilent 1100 Signal Name Shield Shield Analog Center Center Analog ...

Page 190: ...1100 Series Nano Pump Service Manual 4 Parts and Materials Table 32 Agilent 1100 to General Purpose Connector 01046 60105 Pin 3394 6 Pin Agilent 1100 Signal Name 1 Not connected 2 Black Analog 3 Red Analog ...

Page 191: ...t 1100 Series modules The other end depends on the instrument to be connected to Table 33 Agilent 1100 to 3390 Integrators Connector 01046 60203 Pin 3390 Pin Agilent 1100 Signal Name Active TTL 2 1 White Digital ground NC 2 Brown Prepare run Low 7 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start requ...

Page 192: ...w 11 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 9 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low Connector 01046 60210 Pin 3394 Pin Agilent 1100 Signal Name Active TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 5 14 7 Red Ready High...

Page 193: ...wise the integrator prints START not ready NOTE START and STOP are connected via diodes to pin 3 of the 3394 connector Connector 03394 60600 Pin 3394 Pin Agilent 1100 Signal Name Active TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request...

Page 194: ...n Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Connector 5061 3378 Pin HP 1050 Pin Agilent 1100 Signal Name Active TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connecte...

Page 195: ...igital ground NC 2 Brown Prepare run Low 4 3 Gray Start Low 7 4 Blue Shut down Low 8 5 Pink Not connected NC 6 Yellow Power on High 3 7 Red Ready High 6 8 Green Stop Low NC 9 Black Start request Low Connector 01046 60201 Pin Universal Pin Agilent 1100 Signal Name Active TTL 1 White Digital ground 2 Brown Prepare run Low 3 Gray Start Low 4 Blue Shut down Low 5 Pink Not connected 6 Yellow Power on H...

Page 196: ...d depends on the instrument to be connected to Table 41 Agilent 1100 to 3392 3 Integrators NOTE The BCD output for the well plate sampler does not work with the integrators 3392 3 6 Connector 18584 60510 Pin 3392 3 Pin Agilent 1100 Signal Name BCD Digit 10 1 BCD 5 20 11 2 BCD 7 80 3 3 BCD 6 40 9 4 BCD 4 10 7 5 BCD 0 1 5 6 BCD 3 8 12 7 BCD 2 4 4 8 BCD 1 2 1 9 Digital ground 2 15 5 V Low 6 Key ...

Page 197: ...erials 4 Table 42 Agilent 1100 to 3396 Integrators Connector 03396 60560 Pin 3392 3 Pin Agilent 1100 Signal Name BCD Digit 1 1 BCD 5 20 2 2 BCD 7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCD 0 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5 V Low ...

Page 198: ... 81600 Wire Color Pin Agilent 1100 Signal Name BCD Digit Green 1 BCD 5 20 Violet 2 BCD 7 80 Blue 3 BCD 6 40 Yellow 4 BCD 4 10 Black 5 BCD 0 1 Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Pink 10 BCD 11 100 Red Blue 11 BCD 10 200 White Green 12 BCD 9 400 Brown Green 13 BCD 8 800 14 n c 15 n c ...

Page 199: ... a modular plug to be connected to the Agilent 1100 Series vacuum degasser The other end is for general purpose Table 44 Agilent 1100 Series Degasser to general purposes Connector G1322 81600 Color Pin Agilent 1100 Signal Name White 1 Ground Brown 2 Pressure signal Green 3 Yellow 4 Grey 5 DC 5 V IN Pink 6 Vent ...

Page 200: ...AN Cable Both ends of this cable provide a modular plug to be connected to Agilent 1100 Series module s CAN bus connectors Agilent 1100 module to module 0 5m lg 5181 1516 Agilent 1100 module to module 1m lg 5181 1519 Agilent 1100 module to control module G1323 81600 ...

Page 201: ... Table 45 Agilent 1100 Series Interface Board to general purposes 5 10 15 1 11 6 Connector G1103 61611 Color Pin Agilent 1100 Signal Name White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connect...

Page 202: ... 9 pin female Null Modem printer cable and one adapter Use the cable and adapter to connect Agilent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent 1100 module to PC Table 46 RS 232 Cable Kit 34398A QVWUXPHQW 5 7 75 1 65 576 76 5 5 7 75 1 65 576 76 5 3 0DOH HPDOH HPDOH 0DOH ...

Page 203: ...erials 4 LAN Cables Recommended Cables For point to point connection not using a network hub use a twisted pair cross over LAN cable P N 5183 4649 10 feet long For standard network connections using a hub use category 5 UTP cables P N G1530 61480 8 m long ...

Page 204: ...192 1100 Series Nano Pump Service Manual 4 Parts and Materials ...

Page 205: ...ano Pump Introduction to the Nano Pump 194 Electrical Connections 202 Instrument Layout 204 Early Maintenance Feedback EMF 205 The Electronics 207 Firmware Description 213 Optional Interface Boards 215 Interfaces 218 Setting the 8 bit Configuration Switch 223 The Main Power Supply Assembly 228 ...

Page 206: ...low produced by the two pumping units is proportioned in an electomagnetical proportional valve EMPV The remaining column flow is measured in a mass flow sensitive flow sensor The measured flow is compared with the user entered column flow setpoint The flow sensor controls the EMPV current causing the EMPV to correctly proportion the column flow The primary flow in excess of the required column fl...

Page 207: ...re Delivery Solvent Compressibility Compensation Variable Stroke Volume Column Flow Measurement and Control Low pressure solvent metering and high pressure solvent delivery are accomplished by two pump channels each capable of delivering a maximum of 2 5 ml min flow at up to 400 bar pressure Figure 37 Overview of the Nano Pump NPM board Pump drive Outlet valve Leak sensor Power supply Active inlet...

Page 208: ...hich sends system pressure information to the user interface This output flow called primary flow is connected to the Electronic Flow Control EFC system The EFC system consists of an Electro Magnetic Proportioning Valve EMPV in series with a Flow Sensor The EMPV is protected from particles in the mobile phase by a solvent filter frit Responding to user entered column flow setpoint the EFC system d...

Page 209: ...ives in opposite directions 180 degree out of phase The gear ratios are designed such that the first ball screw drive constantly moves at twice the speed of the second ball screw drive The servo motor includes a high resolution shaft position encoder which continuously reports the speed and direction of the motor in real time This speed and direction information is used by the pump control electro...

Page 210: ...he pump The initialization routine occurring for both pump heads first determines the precise movement limits for both pistons These limits are then stored in the pump controller memory Then both pistons are set to their default initial positions When pumping begins the active inlet valve is opened and piston 1 begins its intake stroke sucking solvent into chamber 1 At the same time piston 2 begin...

Page 211: ...h mobile phase Pump head SST gold sapphire ceramic Active Inlet Valve SST gold sapphire ruby ceramic PTFE Outlet Valve SST gold sapphire ruby tantalum Adapter SST gold EMPV SST ruby sapphire PEEK Flow Sensor SST Damping Unit Gold SST Capillaries Fused Silica From solvent bottle Inlet valve Outlet valve Tomixing chamber Seal Piston Ball screw drive Motor with encode Gear ...

Page 212: ...sibility compensation value for a pump head is set the pump processor calculates a compensation volume that depends on the system pressure and the selected compressibility value This compensation volume is added to the delivery stroke of the first piston How Does Variable Stroke Volume Work Due to the compression of the pump chamber volume each piston stroke of the pump will generate a small press...

Page 213: ...disabled This mode is for non capillary LC applications In the micro mode the flow sensor measures and controls column flow in the range of 0 1 µl min to 4 µl min Flow measurement is based on the principle of mass flow temperature sensitivity The flow sensor consists of a heated tube with two temperature sensors As the mobile phase passes through the heated tube the temperature characteristic dist...

Page 214: ...o control the nano pump from a computer via RS 232 connection using appropriate software This connector needs to be activated by the configuration switch module next to the GPIB connector The software needs the appropriate drivers to support this communication See your software documentation for further information The power input socket accepts a line voltage of 100 240 volts AC 10 with a line fr...

Page 215: ...ctions to the Nano Pump GPIB Security Lever Configuration switch Power Plug RS232 Remote Analog output Slot for interface board CAN Bus WARNING To disconnect the nano pump from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off ...

Page 216: ...used in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers...

Page 217: ...the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The nano pump provides a series of EMF counters for the left and right pump heads Each counter increments with pump use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been ...

Page 218: ...user requirements The wear of pump components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When performance indicates maintenance ...

Page 219: ...B or RS 232C connected to the user interfaces Figure 41 and Figure 42 show block diagrams of this board ASIC Application Specific Integrated Circuit The application specific integrated circuit ASIC includes all digital logic for the core processor functions and also for module specific functions Motor Drive There are two identical motor drives for the drive assemblies Each drive comprises motor co...

Page 220: ...n resistance results in a leak signal Fan Drive The revolution of the fan is controlled by the main processor depending on the internal heat distribution in the nano pump The fan provides a PWM signal which is proportional to the revolution This fan status signal is used for diagnostics Electronic Fuses The valve circuits are electronically fused on the board Any error on the board or shortages of...

Page 221: ...o the Nano Pump 5 one GPIB connector as interface to the Agilent ChemStation one RS 232C as interface to a computer one REMOTE connector as interface to other Agilent products one analog output for pressure signal output and one optional interface board LAN ...

Page 222: ... B Drive B Current control B Active inlet valve B Valve drive B SSV A SSV B Active inlet valve A Valve drive A 4 valve drives Drive A Motor A Encoder A Motor control A ASIC J 2 Control Data address Data address Control Motor drive B Motor drive A Processor 1 J Motor control B Current control A EMPV Pump head A Pump head B Option Drive ...

Page 223: ...rd ϑ Multiplexer A D converter Filter amplifier Offset correction Analog output Pressure Damper Amplifier ambient measurement Drive Fan Leak sensor Integrator Senso Pressure converter Control signal Data address Diagnose signals digital Diagnose signals analog 1 2 1 1 Temperature of hybrid Bd temperature control Flow ...

Page 224: ...B Motor drive A Inlet valve drive A SSV valve drive B SSV valve drive A Electronic fuse Electronic fuse Electronic fuse Electronic fuse Hardware switch Hardware switch Leak converter Fan drive Damper converter CSM power supply Leak sensor Fan Damper Analog Power supply 36V P20 P8 P21 P12 P14 P11 P22 P15 P17 P16 J6 P2 15V 5V 24V 24V 24V 24V 24V 24V 24V 24V 24V 15V 15V 15V 15V 36V Processor 36V 36V ...

Page 225: ...pabilities GPIB CAN LAN and RS 232C memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities GPIB CAN LAN and RS 232C memory management ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like run synchronization v...

Page 226: ...e file naming conventions are xxxx vvv DLB where xxxx is the product number e g 2226 for the G2226A Nano Pump and vvv is the revision number for example 503 is revision 5 03 For instructions refer to your user interface NOTE Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Figure 44 Firmware Update Mechanism Resident Sy...

Page 227: ...100 Series well plate sampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused There is a general purpose cable available to connect the BCD output see BCD Cables on page 184 and the external outputs see External Contact Cable on page 189 to external devices Table 48 Optional Interface Boards Description Part Numbe...

Page 228: ...ure 46 Block Diagram BCD Board Vial 1 Vial 10 Pos P2 P24 Vial 985 Plate 2 Pos P2 A1 Vial 601 Pos P2 B1 Vial 625 Pos P1 H12 Vial 297 Pos P1 A1 Vial 201 Pos P1 B1 Vial 213 Plate 1 Processo r Board identificatio BCD register External contacts Line driver 12 4 x 250 mA BCD connector External contact RFI filter RFI filter ...

Page 229: ...P cables P N G1530 61480 8 m long NOTE One board is required per Agilent 1100 stack It is recommended to add the LAN board to the detector with highest data rate NOTE The LAN board can only be used together with a main board version G13XX 66520 DAD MWD VWD Pump ALS or G13XX 66500 FLD RID and above an Agilent ChemStation software revision A 06 01 or above Table 49 LAN Boards Agilent Order Number Su...

Page 230: ...lent 1100 Series Interfaces Interface Type Pumps Autosampler Well plate Sampler DA Detector MW Detector FL Detector VW Detector RI Detector Thermostatted Column Compartment Vacuum Degasse r CAN Yes Yes Yes Yes Yes Yes No GPIB Yes Yes No Yes Yes Yes No RS 232C Yes Yes Yes Yes Yes Yes No Remote Yes Yes Yes Yes Yes Yes Yes Analog Yes No No 2 1 No Yes Interface board Yes Yes Yes Yes Yes No No The well...

Page 231: ...e GPIB address of your module The switches are preset to a default address and recognized by the operating software from Agilent Technologies CAN Interface The CAN is an intermodule communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement NOTE A chemstation cannot be connected directly to the well plate sampler by GPIB Table 51 Def...

Page 232: ...properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level is defined as standard TTL levels 0 V is logic true 5 V is false fan out is...

Page 233: ...ted to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle...

Page 234: ...ump Service Manual 5 Introduction to the Nano Pump 9 In RI Figure 47 RS 232 Cable Table 53 RS 232C Connection Table continued Pin Direction Function QVWUXPHQW 5 7 75 1 65 576 76 5 5 7 75 1 65 576 76 5 3 0DOH HPDOH HPDOH 0DOH ...

Page 235: ...nt specific initialization procedures Switches 1 and 2 define which set of parameters for example for GPIB RS 232C and so on will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory Figure 48 8 bit Configuration Switch Table 54 8 bit Configuration Switch Mode Select 1 2 3 4 5 6 7 8 GPIB 0 0 GPIB Address RS 23...

Page 236: ...just want to change the GPIB address and need a detailed procedure refer to the Installing Your Agilent ChemStation System handbook Default GPIB address is set to the following addresses where 0 means that the switch is down and 1 means that the switch is up Communication Settings for RS 232C Communication The communication protocol used in this instrument supports only hardware handshake CTS RTS ...

Page 237: ...nt to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Table 56 Communication Settings for RS 232C Communication Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baud rate Data Bits Parity Table 57 Baud Rate Settings Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1...

Page 238: ...If you use the following switch settings and power the instrument up again a forced cold start has been completed To return to normal operation set switches back to your GPIB or RS 232 configuration settings Stay Resident Settings Firmware update procedures may require this mode in case of firmware loading errors 1 0 Odd Parity 1 1 Even Parity Table 59 Parity Settings continued CAUTION Forced cold...

Page 239: ...normal operation If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident part that is it is not operable as a detector It only uses basic functions of the operating system for example for communication To return to normal operation set switches back to your GPIB or RS 232C configuration settings Table 61 Stay Resident Settings Mode S...

Page 240: ...he detectors The line voltage can vary in a range from 100 240 volts AC 10 and needs no manual setting To disconnect the instrument from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off Figure 49 Main Power Supply MPS Block diagram primary circuits 24 V supply 5 V switching regulator linear voltage regulators 12 V supply fl...

Page 241: ...urn off output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrument off wait until it is approximately at ambient temperature and turn the instrument on again The following table gives the specifications of the main power supply Table 62 Main Power Supply Spe...

Page 242: ...230 1100 Series Nano Pump Service Manual 5 Introduction to the Nano Pump ...

Page 243: ...hnologies 6 Control Module Screens for the Nano Pump Major keys on the Agilent 1100 Control Module 232 Screens available from the Analysis view 233 Screens available from the System view 245 Screens available from the Records screen 248 Diagnostics and Tests 254 ...

Page 244: ...ivate settings of current screen On Off Switch on individual Instrument s or complete System Start Start a location range or sequence Plot View online signals Views Change between analysis samples status system views NOTE The screens shown on the next pages are based on the following firmware revisions Control Module firmware revision B 02 0x G1323B HPLC Module firmware revision 5 0x NOTE In case ...

Page 245: ...lent 1100 module It is used to enter the most common pump method parameters The m key allows access to the context sensitive menus Date Time allows you to change time settings Print Screen gives access to the print screen About tells you the current firmware revision and the serial of your control module Setup view leads you to analysis view configuration for additional Agilent 1100 modules Restar...

Page 246: ... thermostatted column compartment parameters are shown on the display as well The number of parameters for each module is reduced as additional modules are added to the display A maximum of 4 modules can be shown simultaneously If more modules are connected to the system you can choose up to 4 of them in Setup view On Off From the Analysis screen use the F7 key On Off to proceed to the turn on scr...

Page 247: ...p Service Manual 235 Control Module Screens for the Nano Pump 6 Settings With the F1 key Settings you open a pull down menu where you can select the capillary pump module with the help of the selection or alphanumeric keys ...

Page 248: ...tion you have access to a different set of parameters available through the F1 5 navigation keys The F7 key Default resets the pump to default values F8 On Off opens a window to turn on the pump Changes must be acknowledged with F6 Done Use the m key for the context sensitive menu Reset will load the pump default parameters The Status command pulls up the module specific setup screen Use the F1 ke...

Page 249: ... binary solvent mixtures Please note that the left mobile phase in the line must be pumped from channel A the right one must be connected to channel B Changes must be activated with F6 Done Select Channel Selection to choose one of the A channels 1 or 2 and one of the B channels 1 or 2 as the sources for solvent delivery Changes must be activated with F6 Done ...

Page 250: ...nual 6 Control Module Screens for the Nano Pump Settings More Select Auxiliary to access the pumps performance optimization parameters Compressibility and Minimum Strike for each channel individually Changes must be activated with F6 Done ...

Page 251: ...tivated with F6 Done Select Mode to toggle between Micro Flow and Normal Flow Changes must be activated with F6 Done Settings Timetable With the F2 key Timetable you can list and edit the timetable for the pump Press F7 key Insert to add entries F6 key Delete to remove or Enter to edit selected highlighted entries NOTE The following screen is only available in Micro Flow mode see below ...

Page 252: ...ne Use the pull down menus if available Use the F6 key Done to view the entered lines of the timetable or F7 Enter to acknowledge current settings Use the m key for the context sensitive menu It gives you additional tools for the timetable Settings Pressure With the F3 key Pressure you can change the settings for the pressure limits ...

Page 253: ...ill to refill All bottles or a Selected bottle to total volume By selecting the respective tick boxes you can also choose whether and when to have Not Ready or Error conditions Changes must be activated with F6 Done Settings Runtimes With the F5 key Runtimes you can change the stop time and the post run time for this module individually Changes must be activated with F6 Done Analysis Status Press ...

Page 254: ...ate mobile phase composition pressure and Ripple elapsed run time and the pressure plot are shown Press key F8 Start to start a run key F7 Rescale to maximize the signal Signal plot Press F6 key Plot to enter the plot screen available also from all other views Here you can observe online signal s If more than one signal is configured use the 1 2 3 numeric keys to switch between the signals ...

Page 255: ...grams or temperature signals from other modules Use the Right Left selection keys to switch between Available Signals and Selected Signals Use the F8 key Move or Enter to move available signals into the box for selected signals or vice versa Use F7 to Setup ranges for each individual highlighted signal Press F6 Done to activate the changed settings and to return to the Plot screen ...

Page 256: ... is only active when a PCMCIA card is inserted in the control module Use F2 key PC Card to transfer a method from to a PCMCIA card Use the Right Left selection keys to switch between PC Card and Instrument window Use the Up Down selection keys to select the method Use the F7 F8 keys Copy or Enter to move available methods between PCMCIA card and Modules F6 Deletes a selected method NOTE The PCMCIA...

Page 257: ...no Pump 6 Screens available from the System view System view Use the Esc key to until you receive Views on the F5 key Choose System from the pull down menu This screen shows the last activities in the system System Control Use the F1 key Control and select the capillary pump ...

Page 258: ... information about the not ready conditions if needed Use F1 Purge Task to access the pump s Purge Task After selecting a channel you can edit its Purge Time and Flow Rate Use F8 Purge to start stop the Purge Task as defined or define the settings for the next Purge Task Changes must be activated with F6 Done System configuration On the System screen use the F2 key Configure and select the pump ...

Page 259: ...Pump 6 This screen gives you information about installed options and allows you to configure your pump to the filter and mixer type that is actually installed in the pump Changes must be activated with F6 Done Press F2 to edit and enable disable Immediate Fast Composition Change ...

Page 260: ...Use the F4 key Records and select the pump Errors are reported either into the F2 System Log or F3 Error Log EMF Early Mainte nance Feedback Use the F1 key EMF to set EMF Limits Choose menu item Setup Limits to set the amount of pumped solvent or seal wear when you want to receive a warning for exceeded limits Press F7 Reset to reset the counters after exchanging parts that are subject to wear Cha...

Page 261: ... box will pop up If you press Reset the limits will be removed Ignore will continue to keep the EMF flag set System Error Log Use the F2 key System Log or F3 key Error Log to look for errors on the selected highlighted module NOTE An exceeded limit will not stop a sequence or run information only to plan maintenance activities ...

Page 262: ...ce Log Use the F4 key Maint Log to review and enter maintenance activities in the maintenance logboo0k Use the F7 key Add to add new maintenance activities You can add a comment to the activity into the line Add using the control modules alphanumeric keys Changes must be activated with F6 Done ...

Page 263: ... RESnnnn DLB and press execute If you want to update the main firmware press F7 key Transfer to turn the module into the resident mode LED on module should blink yellow Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F3 key Records to select the Generic module In this screen the resident firmware revision is shown Use the F5 key FW Update to enter the U...

Page 264: ...overwritten during the update process Changing the serial number In case the serial number of the module has to be added use the m key to open the menu Enter Serial The serial number becomes active after restart of the module Type in the module s Serial as indicated on the front or rear label of the module using the alphanumeric keys Changes must be activated with F6 Done ...

Page 265: ...1100 Series Nano Pump Service Manual 253 Control Module Screens for the Nano Pump 6 ...

Page 266: ...ests screen Use the Esc key until you receive Views on the F5 key Choose System from the pull down menu Use the F3 key Tests to select the pump Calibration Press F1 Calibrate to access the pump s calibration screen Select one of the calibration curves stored on the module with the help of the selection keys ...

Page 267: ...Load Save a calibration curve from to the PCMCIA Card by pressing F8 PC Card Leak Test Press F2 Leak Test on the Test screen to perform a leak test Several steps like purging the system setting up Isopropanol as solvent on the channel that is supposed to be tested and blocking the purge valve outlet with a blank nut have to be performed before operating the leak test For details use the i key to a...

Page 268: ...sopropanol as solvent on channel A or A2 if solvent selection valve is installed and blocking the column outlet with a blank nut have to be performed before operating the pressure test For details use the i key to achieve context sensitive help follow the instructions on the screen and refer to Running the Normal Mode Pressure Test on page 69 For evaluating the pressure test refer to Evaluating th...

Page 269: ...gilent 1100 Series Nano Pump Service Manual Agilent Technologies 7 Specifications Performance Specification Agilent 1100 Series Nano Pump 258 Performance Specification Agilent 1100 Series Micro Degasser 259 ...

Page 270: ...primary flow pump channel is 5 µl min Delay volume Typically 300 nl from the electronic flow control to the pump outlet for flow rates up to 4 µl min For flow rates up to 4 µl min and electronic flow control active primary flow path 180 480 µl system pressure dependent default settings calculated volume Typically 180 to 480 µl system pressure dependent for flow rates up to 2 5 ml min default setti...

Page 271: ...n terms of seal wear and volume of pumped mobile phase with user settable limits and feedback messages Electronic records of maintenance and errors Housing All materials recyclable Table 64 Performance Specification Agilent 1100 Series Micro Degasser Type Specification Maximum flow rate 5 ml min per channel Number of channels 4 Internal volume Typically 1 ml per channel Internal design One chamber...

Page 272: ...260 1100 Series Nano Pump Service Manual 7 Specifications ...

Page 273: ...68 Agilent Technologies on Internet 269 The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer...

Page 274: ...onal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuseholders must be avoided Do not operate the...

Page 275: ...rized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting ...

Page 276: ... by the Xenon flash lamp used in this product Always turn the xenon flash lamp off before removing it WARNING A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met CAUT...

Page 277: ...ATA ICAO ADR RID IMDG is not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries WARNING Lithiumbatteri Eksplosionsfare ved fejlagtic handtering Udskiftning ma kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverandoren WARNING Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes ku...

Page 278: ...ogies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises ...

Page 279: ...This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position 70 dB Sound Pressure Lp 70 dB A At Operator Position Normal Operation According to ISO 7779 1988 EN 27779 1991 Type Test ...

Page 280: ...stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl3 O2 2COCl2 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filte...

Page 281: ...t Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1100 series modules for download ...

Page 282: ...270 1100 Series Nano Pump Service Manual A Safety Information ...

Page 283: ...141 damping unit 141 degreaser spray 122 delay volume 7 delivery checklist 5 description leak test 73 dimensions 3 disassembling the pump head 120 E early maintenance feedback EMF 204 electrical connections 202 electronic fuses 202 208 electronics HPM board 207 electrostatic discharge ESD 105 EMF on control module 251 EMF counter 205 EMF flag 205 EMF limits 206 EMPV cleaning procedure 91 EMPV part...

Page 284: ... board 134 humidity 4 hydraulic path parts 161 hydraulic system 258 I index adjustment 60 index limit 59 index missing 61 indicator power supply 23 initialization failed 63 injection first 17 inlet valve fuse 47 inlet valve missing 52 installation pump module 12 instrument status indicator 24 interface board 129 interface board BCD LAN 215 interfaces 208 analog signal output 219 APG remote 220 CAN...

Page 285: ...ults pressure test 71 RS 232 cable kit to PC 190 RS 232C communication settings 224 interface 221 settings 225 RS 232C connector 202 run mode 24 running the leak test 74 running the pressure test 69 S safety standards 4 safety information 261 on lithium batteries 265 safety light switch 154 screwdriver pozidriv 1 118 126 141 143 145 147 149 152 154 seal wear counter 123 206 seals 108 security leve...

Page 286: ...274 1100 Series Nano Pump Service Manual Index wrench 14 mm 109 112 114 115 117 134 149 wrench 5 mm 134 149 wrench 7 mm 134 149 Z zero solvent counter 39 ...

Page 287: ......

Page 288: ...e information about the Agilent 1100 Series Nano pump The manual describes the following installation optimizing performance diagnostics and troubleshooting repairing parts and materials introduction to the pump theory of operation screens of the local control module and specifications ...

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