Advanced Bilirubin Stat-Analyzer BR2, Service Manual, Page: 16 / 69

16
The Advanced® Model BR2 Service Manual
NOTE
When the instrument is first turned
on, the measuring circuit requires
thirty minutes’ warm-up (to avoid
frequent re-zeroing to compensate
for initial thermal drift). It should be
left on during STAT duty (electrical
consumption is less than 20 watts).
Calibration Procedure
Verify the stated bilirubin values on the labels
of calibration standards and control sera,
preferably by using an acceptable manual
method. Record the actual values. Then cali-
brate the BR2:
1. Press the Total button.
2. Pipette 1 mL of pH-adjusted saline diluent
from an Advanced Bilirubin Test Kit into
one of the cuvettes provided. Open the
cuvette chamber and load the cuvette into
it so that the light beam passing from right
to left passes through the
clear , not the
ribbed sides. Close the cuvette chamber
cover against extraneous light.
3. Adjust the readout to 00.0 with the Zero
knob.
4. Add 30 µL of calibration standard or con-
trol serum to the saline diluent in the
cuvette and mix thoroughly.
NOTE A positive-displacement pipette is
recommended for optimum accu-
racy in pipetting these fluids; at
high TBR concentrations, small
pipetting errors can lead to signif-
icant falsely-low readings. It is
further suggested that the same
pipettes be used each time, to
assure sample-to-sample and day-
to-day consistency.
5. Adjust the readout as necessary, by means
of the Calibrate knob, to the actual value
of the standard or control serum used (as
determined in step 0 above). Repeat
steps 0 through 4 with enough additional
aliquots to verify instrument calibration.
The BR2 is now calibrated and ready for
operation with unknown bilirubin samples.
Calibration