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Anoxomat® III User’s Guide

Foreword

Intended use

The Anoxomat uses the widely known McIntosh and Fildes system of evacuation and replacement to create anaerobic 
environments. The Anoxomat® quickly creates anaerobic, microaerophilic, or capnophilic and hypoxic environments to 
cultivate bacteria in jars using a reliable automated evacuation/replacement method. The Anoxomat® can create exact, 
repeatable conditions with gas mixtures within 0.5% of the required value.

The Anoxomat® is designed to cultivate oxygen-sensitive organisms in anaerobic jars using a system that reliably sustains 
environments for proper growth. The Anoxomat is useful to the following disciplines:

• 

Microbiological and bacteriological research

• 

Clinical microbiology laboratories

• 

Medical research

• 

Biotechnology research and manufacturing

• 

Pharmaceutical research and manufacturing

• 

Food safety microbial testing

• 

Environmental monitoring

• 

Academic research

• 

Industrial applications

Summary of Contents for Anoxomat III

Page 1: ...Anoxomat III User s Guide AN3005EN Rev 5 ...

Page 2: ...ter 3 Optional functions 17 3 1 Barcode scanner 17 3 2 Registration input screen 17 3 3 Pre programmed recipes 17 3 4 Additional jar connections 18 3 5 Additional gas connections 18 3 6 User programming function 18 3 7 Activating pre programmed or user defined recipes 20 3 8 Thermal dot matrix printer 20 3 9 Data Interface 20 Chapter 4 Maintenance 22 4 1 Maintaining the Anoxomat 22 4 2 Maintaining...

Page 3: ...r to the presence of important operating and maintenance servicing instructions in the literature accompanying this product The lightning flash with arrowhead symbol within an equilateral triangle is intended to alert the user to the presence of uninsulated dangerous voltage within the product s enclosure that may be of sufficient magnitude to constitute risk of electric shock to persons The stati...

Page 4: ...short term temperature variations or locations that exceed the specified operating environment temperatures Never use this product in a wet area To avoid injury or fire hazard do not operate this product in an explosive atmosphere Do not install or use the product on an unstable non level work surface Do not operate this product with the covers removed or unsecured FCC requirements WARNING Changes...

Page 5: ... can create exact repeatable conditions with gas mixtures within 0 5 of the required value The Anoxomat is designed to cultivate oxygen sensitive organisms in anaerobic jars using a system that reliably sustains environments for proper growth The Anoxomat is useful to the following disciplines Microbiological and bacteriological research Clinical microbiology laboratories Medical research Biotechn...

Page 6: ...prong electrical outlet capable of continuously supplying 2 amperes at 220V and 5 amperes at 100 110V If the instrument is not grounded properly its operation may be impaired and a safety hazard may exist Therefore be sure to test the outlet and record the results before operating your instrument 1 2 Additional items needed for installation Adjustable wrench to tighten the dual stage regulator 5 8...

Page 7: ...card at aicompanies com warranty Anoxomat III and accessories 1 4 Installing the gas supply To use the Anoxomat you ll need to connect at least one gas cylinder with a standard gas mixture used for anaerobic as well as for microaerophilic bacteria cultivation see Standard gas mixture on page 11 Before you begin make sure the gas cylinder valve is off until the gas supply is fully installed Caution...

Page 8: ...panel This image is for reference only Your Anoxomat system may have different connections This 6 Open the gas cylinder s main supply and set the regulator gas pressure to 1 75 15 bar 25 5 psi 2 psi 7 Repeat for each gas connection Connecting the gas supply to a 100V or 110V system See Installing the gas supply before you begin 1 Mount the regulator on the gas cylinder using the adjustable wrench ...

Page 9: ...twork optional If your Anoxomat includes a data interface to a computer or network Use a cross link Ethernet cable to connect the Anoxomat directly to the network card of the computer Use a standard patch cable to connect to a network See instructions for Activating the data interface optional on page 13 1 7 Power up the instrument 1 Connect the power cord to the Anoxomat and into a power outlet 2...

Page 10: ...Fig 1 1 and press Ok The main Setup screen displays Set up gas connection s The Anoxomat allows a maximum of three gas supply connections For each gas connection you need to specify the gas mixture on the Setup screen For Gas 1 select the gas composition of your gas from the corresponding pull down menu Specify the gas composition for each gas supply See Standard gas mixture on page 11 If your gas...

Page 11: ...d for the 100 CO2 cylinder and the third gas connection gas 3 is used for the H2 mixture cylinder WARNING Gas mixtures with hydrogen greater than 10 are considered flammable Gas mixtures with concentrations of oxygen above the normal 21 atmospheric significantly increases the risk of ignition and fire Never use such hazardous gas mixtures with the Anoxomat For other jar composition results see Sta...

Page 12: ...ient plates are scanned and processed in the jar Register users To limit access to the Anoxomat user IDs can be established to identify the technician before every recipe is run 1 On the Registration screen press Register Users 2 In the Add user section of the screen enter the user s full name as you want it to appear on the printout and in the database 3 Enter the user code for the user The user ...

Page 13: ...n the Setup screen press Interface 2 Enter the Data Interface Code from the red label Fig 1 1 and press Ok The Network Settings screen appears Connecting to a network Use a standard patch cable Ethernet Cable to connect to a network Select Obtain an IP address automatically and reboot the Anoxomat The IP Address and Subnet Mask appears in the network settings Connecting directly to a PC Use a cros...

Page 14: ...hets needed AJ9022 holds 6 plates 1 AJ9023 holds 12 plates 1 AJ9025 holds 12 plates microtiter plates 2 AJ9049 holds 12 plates 1 AJ9050 holds 24 plates 2 AJ9028 holds 36 plates 3 Use the sachet at room temperature Clip the disposable sachet under the standard jar s lid or place it in the ergonomic jar s designated slot Dispose of the sachet in accordance with lab procedures and local biohazard was...

Page 15: ...us run Jar passed the test Jar is ready to be disconnected and placed in the incubator In the Ready column this indicates the process is complete Jar failed the test Processing stops on that jar The jar may be disconnected Active Recipe screen Gas input test When the Gas input test fails this means the pressure supplied by the regulator to the Anoxomat is lower than 1 6 Bar or higher than 1 9 Bar ...

Page 16: ... should snap out of the coupling This closes the snap shut coupling and maintains air tightness of the jar Hold the black tube with your other hand when disconnecting from the jar To leave the process report screen press Start The Anoxomat returns to the Main screen and is ready for the next job The last performed recipe stays active You can check this on the Active Recipe screen ...

Page 17: ...d to input details for each connected jar If you marked any of the fields required during jar setup you cannot continue without completing the respective field Use the screen keyboard to specify details for each jar connection Use the horizontal scroll bar to display other options or use the Barcode scanner if the jars use the barcode labeling system 4 When finished press Start to continue the rec...

Page 18: ...as mixtures can be connected if more gas connections are available Gas Connection Cylinder Contents Usage Primary N2 Nitrogen 85 Standard anaerobic and microaerophilic recipe CO2 Carbon Dioxide 10 H2 Hydrogen 5 Secondary 1 CO2 Carbon Dioxide 100 Increased levels of CO2 Table 3 1 Primary and one secondary gas supply Gas Connection Cylinder Contents Usage Primary N2 Nitrogen 85 Standard anaerobic an...

Page 19: ... the gas in the secondary cylinder More than one solution is possible By changing the position of the scroll bars of Gas 1 and Gas 2 the result of the mixture becomes different 6 Define maximum evacuation level The catalyst test is only performed in case a required oxygen level of 0 has been chosen The No Catalyst Test button should be used when creating a new recipe and a catalyst is a not requir...

Page 20: ... to a web page Use this option to transfer the data into an existing external system e g LIMS software system as a CSV comma separated values file 1 Start your web browser 2 Connect to Anoxomat address Type anox and the serial number without the hyphen character e g anox0312001 in the address bar The serial number can be found on the red protection label Fig 1 1 and in the Anoxomat Setup screen If...

Page 21: ...ecipes Click Filter and choose one or more filter criteria Click Show to display only the filtered recipes To reset the recipe filter Click Reset Forms Confirm Remember to select Save whenever you close the file or any changes or new data is lost View Anoxomat statistics Select the day result sheet to view statistical information about the work done with the Anoxomat The table displays the quantit...

Page 22: ...Teflon in the snap shut coupling to keep the coupling lubricated Clean and apply acid free petroleum jelly on the rubber O ring of the lid only with the standard jars Clean and leave dry no petroleum the seal in the ergonomic jar lids To be certain that the jars are maintained correctly and regularly a small box with necessities to maintain all jars working with the Anoxomat standard jars or Ergon...

Page 23: ...re inspected frequently about once per month for signs of excessive wear Also closely inspect the jar if it fails the Jar Test or Seal Leak Test during processing And finally inspect the jar if the Pump Time Exceeded or Fill Time Exceeded message is displayed Deep horizontal cracks especially near the top are indications that the jar has been dropped or the clamps have been tightened excessively F...

Page 24: ...act with the human body or other conductor may damage system boards or static sensitive devices Never perform internal maintenance without following recommended static protection procedures The product is equipped with operator accessible fuses If a fuse blows it may be due to a power surge or failure of a component Replace the fuse only once If the fuse blows a second time it is probably caused b...

Page 25: ...rument Turn the power switch off and disconnect the power cord Use a small flat bladed screwdriver to pry open the fuse compartment door Slide out each fuse holder Check for a blown fuse Test the fuses with a continuity checker or ohmmeter or replace with new fuses Check error messages The software of your instrument is designed to display specific error messages that help you discover the source ...

Page 26: ...t e g pencil Before reconnecting the jar pull the outer ring of the snap shut coupling downward II Gas Connection Failure Gas connection has not passed the corresponding test No pressure from gas cylinder Check if gas cylinder is open Pressure supplied to Anoxomat should be 1 75 0 15 bar 25 5 2 0 psi Check output pressure on the regulator The regulator gauge does not indicate actual pressure Check...

Page 27: ... black tube including the insert must be replaced Contact your local Anoxomat distributor or Advanced Instruments Leaking jar tube black tube Visual check In case the jar tube is bent cracked or torn contact your local Anoxomat distributor or Advanced Instruments IV Jar Seal Test Failure Error during fill Jar lid not clamped Disconnect jar air out the jar reconnect and process Do not tighten or lo...

Page 28: ...d restart the recipe VI System Errors System does not start up No power Fuses are blown Check plugs Call Service engineer Pump time exceeded Jar was disconnected during evacuation Reconnect the jar Lid of jar was opened during recipe Close and retighten the clamp Pump is losing capacity Pump needs to be serviced Fill time exceeded Enough pressure but not enough gas flow Check for kinks in the gas ...

Page 29: ...the vacuum in the jar by pushing into the snap shut coupling using a pencil Anoxomat has detected a vacuum condition in the jar The jar has been partly processed Before reconnecting the jar pull the outer ring of the snap shut coupling downward Misidentified samples Incorrect sample ID entered manually Use barcode scanner to scan samples or manually recheck ID to reduce transcription errors Softwa...

Page 30: ...nection ANX4J1G JP ANX4J1G 1 ANX4J1G 2 Anoxomat 4 Jar and 1 Gas Connection Part Description Jars AJ9025 Holds one stack of 12 Petri dishes 9 10 cm diameter Holds three Microtiter plates 13 x 9 cm Holds one stack of 12 Petri dishes 15 cm diameter AJ9049 Holds two stacks of 6 Petri dishes 9 10 cm diameter AJ9050 Holds two stacks of 12 Petri dishes 9 10 cm diameter AJ9028 Holds three stacks of 12 Pet...

Page 31: ...S Barcode scanner AN2ISC1 Registration input screen AN2DI Data interface AN2PPR Pre programmed recipes AN2TT Track and trace package AN2UPF User programming function AN2TP1 Recipe printer thermal paper or medical grade paper AN2TP3 Dot matrix printer for plain paper MT2000 Jar maintenance toolkit MT2001 Refill for jar maintenance toolkit AN3146 Palladox Disposable Sachet box of 40 ...

Page 32: ...linder Microaerophilic Anaerobic 10 Liter 150 bar 700 200 10 Liter 200 bar 950 250 50 Liter 150 bar 3600 1000 50 Liter 200 bar 4900 1400 Note Based on 12 plate jar Temperature and Humidity Operating Conditions 10 C to 32 C 50 F to 91 F Humidity 20 80 relative humidity non condensing Storage Conditions 0 C to 70 C 32 F to 158 F Dimensions inches centimeters Width 12 30 5 Depth 9 23 0 Height 13 33 0...

Page 33: ...uirements for Electrical Equipment for Measurement Control and Laboratory Use Part 2 101 Particular Requirements for In Vitro Diagnostic IVD Medical Equipment The C in the lower left of the ETL mark demonstrates these listings Europe Low Voltage Directive This product conforms to 2014 35 EU Low Voltage Directive Europe Electromagnetic Compatibility Directive 2014 30 EU This product conforms to Dir...

Page 34: ...f this warranty in no case include any obligation or responsibility for transportation charges or arrangements All results calibrations effectiveness medical diagnoses and interpretations are the responsibility of the user THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES REPRESENTATIONS AND CONDITIONS OF ANY KIND EXPRESSED OR IMPLIED IN FACT OR BY LAW IN NO EVENT SHALL ADVANCED INSTRUMENTS BE LIAB...

Page 35: ...sonnel if advised immediately by the user of the occurrence of any accident The user warrants that 1 The instrument will be used reasonably 2 The instrument will be regularly maintained according to this manual including a log of all service tests and repairs performed on the equipment and records will be kept of all requests for repair made to Advanced Instruments where such repairs were beyond t...

Page 36: ...dvanced Instruments assuming English reading personnel or supervision If neither the supervisor nor the operator reads English the user agrees to obtain accurate translations of the instrument labels instructions user s guides and or manuals provided Under direct on the job supervision of the supervisor or other professional in charge In which there are no known defects or uncorrected repairs Only...

Page 37: ...nformation The DB 9 RS 232 port on your instrument conforms to the DTE EIA 232 standard and can reliably communicate over shielded cable up to 10 meters in length This port is reserved for communication to the printer software updates and factory testing purposes ...

Page 38: ...e accompanying documentation On off Feed Interrupt Test Start Stop Record review Setup Calibration Cancel Delete Functional arrow Printer Enter RS232 Bar code Attention Caution hot surface Dangerous voltage Lifting hazard CAL Calibrator CONT Content Control CONTROL Negative control CONTROL Positive control Flammable Fragile Irritant Keep dry Date manufactured Sterile ...

Page 39: ...s conformity to relevant European directives Temperature limit See instructions Lot number Use by Expiration date Authorized representative Part number Sufficient for x tests Open here Low fluid level Keep hands clear Manufacturer LATEX Latex free Diluent See instructions for temperature guidelines Potential puncture hazard Indicates the product conforms to the requirements of EU Directive 2012 19...

Page 40: ... decision has been made to replace or dispose of this product Contact the supplier who sold you the product Whether this was Advanced Instruments itself or one of its authorized dealers this supplier should be knowledgeable about the national and local regulations governing disposal and recycling of products in your area In some cases this supplier may be legally obligated to accept the product fr...

Page 41: ...all rights reserved Under copyright laws this guide may not be reproduced in any form in whole or part without the prior written consent of Advanced Instruments Advanced Instruments has reviewed this guide thoroughly All material contained within is believed reliable but the accuracy and completeness are not guaranteed or warranted and are not intended to be representations or warranties concernin...

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