ABX Pentra 80 RAB108EA, User Manual

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Description & Technology
Measuring principles
Pentra 80 - User Manual - RAB108EA
6–15
3.3. WBC and differential count
3.3.1. General counting principles
The WBC count is carried out twice by two different analysis methods which utilize the total
WBC count in both areas:
◆ Once in the BAS count chamber during the same time as the BAS count,
◆ Once in the optical chamber during the same time as the LMNE count.
The WBC reference count is the count which is obtained from the WBC/BAS count chamber.
3.3.2. BAS/WBC Count
Tab. 6–3: WBC/BAS count
▼ Results
WBC : The number of cells counted within a specified amount of time per volume x WBC co-
efficient of calibration.
BAS : The number of cells counted within a specified amount of time per volume x the WBC
calibration coefficient in a percentage as to the total number of leukocytes (Basophils and
WBC nuclei)
The measurement principle is exactly
the same as the RBC/PLT measurement.
The Differentiation between the BASO’s
and the other leukocytes is obtained by
the use of the
ABX BASOLYSE II
reagent
with it specific lysing action.
All the WBCs are counted between the
electronic thresholds from
<0> to
<BA3> .
The basophils are counted between
thresholds
<BA2> and <BA3> .
Fig. 6–17 WBC/BAS histogram
Technical characteristics of the WBC/BAS counts
Initial blood volume 10 µl (CBC or CBC/DIFF) Method Impedance
Vol. ABX BASOLYSE II 2000 µl Aperture diameter 80 µm
Final dilution rate** 1/200 Count vacuum 200 mb
Temperature of reaction 35°C Count period 2 X 6 seconds