6-30
CELL-DYN
®
3200 System Operator’s Manual
9140181K—July 2002
Calibration Procedures
Auto-Cal Method
Section 6
Entering Reference Values
1. To enter reference values for each parameter to be calibrated:
a. If calibrator is used, if necessary, press
[CALIBRATOR]
, then enter the
corresponding reference (assay) value from the sheet enclosed with the
calibrator material.
b. If fresh whole blood is used, if necessary, press
[WHOLE BLOOD]
, then
enter the mean value obtained from the procedure in subsection
Fresh
Whole Blood
under
Determining Reference Values
above.
NOTE:
Enter the WBC mean as the reference value for both WOC and
NOC.
2. Press the Enter key after each entry to save the value and advance the cursor
to the next parameter.
NOTE:
To change a value after it was entered, place the cursor on the
appropriate field and enter the correct value. To clear a value, press
[QUIT]
followed by
[CONFIRM QUIT]
.
3. When all reference values have been entered, press
[START AUTO-CAL]
to
ready the instrument for calibration.
Processing Samples
1. Mix the sample well. If Calibrator, follow the directions on the package
insert. If Fresh Whole Blood, invert the sample ten times. Do not shake the
specimen. Place the well-mixed specimen in the Tube Retainer, close the
door, and press the Touch Plate. The instrument performs RUN 1 and
displays the values in the RUN 1 row.
2. Repeat step 1 above until the desired number of runs for either Calibrator or
Fresh Whole Blood has been completed.
Calibration Check
During the RUN cycle, if a parameter fails the internal reference/precision checks
for calibration, the value for that parameter is backlit in green on the run line, dashes
are displayed in the Current Factor field, and the Mean Factor is not updated.
Determining Which Parameters Need Calibration
1. After the
[ACCEPT]
key is displayed, you may continue processing samples
until the desired number of runs has been completed.
2. Observe the Factor % Diff value for each parameter being calibrated:
a. If the value is not highlighted (remains uncolored), that parameter does
not need calibration and the new calibration factor (Mean Factor)
displayed on the screen will not replace the existing calibration factor
when the
[ACCEPT]
key is pressed. An uncolored value indicates the
Factor % Diff is less than the value shown in column 2 of Worksheet 5,
Mode to Mode Calibration Range Criteria, provided in
Manual
Calibration Worksheets
at the end of this section.
