6-26
CELL-DYN
®
3200 System Operator’s Manual
9140181K—July 2002
Calibration Procedures
Auto-Cal Method
Section 6
Auto-Cal Procedure
¥
Open Mode
Displaying the Auto-Cal Screen
1. If necessary, go to the
RUN
screen and press
[CHANGE SAMPLER]
to select the
Open Mode.
2. In the
MAIN MENU
, press
[CALIBRATION]
to display the main
CALIBRATION
menu.
3. If necessary, press
[OPEN SAMPLER]
to display the Open Sampler Calibration
Factors.
NOTE:
This key is used to display either the current Open Sampler Factors
or Closed Sampler Factors. It does not change the mode.
4. Press
[PRINT]
to obtain a printout of the current Open Sampler Calibration Factors.
5. Press
[AUTO-CALIBRATE]
to display the
AUTO-CALIBRATION
screen.
Entering Reference Values
1. To enter reference values for each parameter to be calibrated:
a. If calibrator is used, if necessary press
[CALIBRATION]
, enter the
corresponding reference (assay) value from the sheet enclosed with the
calibrator material.
b. If fresh whole blood is used, if necessary press
[WHOLE BLOOD]
, enter the
mean value obtained from the procedure in subsection
Fresh Whole
Blood
under
Determining Reference Values
above.
NOTE:
Enter the WBC mean as the reference value for both WOC and
NOC.
2. Press the Enter key after each entry to save the value and advance the cursor
to the next parameter.
NOTE:
To change a value after it was entered, place the cursor on the
appropriate field and enter the correct value. To clear a value, press
[QUIT]
followed by
[CONFIRM QUIT]
.
3. When all reference values have been entered, press
[START AUTO-CAL]
to
ready the instrument for calibration. The message
Ready to aspirate
Cal sample
is displayed.
Processing Samples
1. Specimen preparation:
a. If using calibrator material, prepare the calibrator for use according to the
directions given in the package insert. Be certain to carefully read and
follow directions given for warming and mixing.
b. If using fresh whole blood, mix the sample well by inverting the tube at
least ten times. Do not shake the specimen.
2. Place the well-mixed specimen under the Open Mode Sample Probe and
press the Touch Plate. The sample is analyzed and the results are displayed.
3. Repeat steps 1 and 2 above until the desired number of runs for either
Calibrator or Fresh Whole Blood has been completed. Mix the sample well
between each run.
