CELL-DYN
®
3200 System Operator’s Manual
6-19
9140181H—October 2001
Section 6
Calibration Procedures
Auto-Cal Method
Overview
The Auto-Cal program provides an automated calibration method that prepares the
CELL-DYN 3200 System for calibration, calculates new calibration factors, and
calibrates the instrument.
NOTE:
Always complete the Pre-Calibration procedures before beginning any
calibration.
Methodology
The instrument should be calibrated in both Open and Closed Modes using either
the CELL-DYN Calibrator or normal, fresh whole blood.
NOTE:
Auto-Calibration is not available in the Closed Mode on the SL model. To
calibrate Closed Mode, see
Calibration Procedure — Mode to Mode
in
subsection
Enter Factor Method
.
The same reference values and the same samples should be used when calibrating
both modes. Either mode may be calibrated first, but it is recommended to calibrate
the primary mode first.
NOTE:
Calibrator is the preferred material for calibrating the CELL-DYN 3200
System.
When samples are run, Auto-Cal does the following:
1. Accepts up to ten consecutive sample runs for either calibrator or fresh whole
blood samples.
2. Compares sample results against internal precision and reference checks,
highlighting results that fail these checks.
3. Calculates the new calibration factors (Mean Factors) and Factor % Diff
values.
4. Compares the Factor % Diff values to ranges in an internal table to determine
which parameters require calibration.
NOTE:
These ranges are shown in the Calibration Range Criteria
worksheets (Worksheet 3 for Open Mode and Worksheet 6 for
Closed Mode) in
Manual Calibration Worksheets
at the end of this
section.
5. Highlights Factor % Diff values which require calibration or which are over-
limit.