6-10
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Calibration Procedures
Pre-Calibration Procedures
Section 6
CELL-DYN 3200
PRE-CALIBRATION PROCEDURES CHECK LIST
Instrument:
Date:
Operator:
1._______ Perform all required maintenance.
2._______ Verify that all reagent containers are at least 1/3 full and the waste container is less than 1/2 full.
3._______ Verify that the reagents have not reached the expiration date.
Diluent/Sheath:
Lot #____________
Exp. date _______
HGB Lyse:
Lot #____________
Exp. date _______
WBC Lyse:
Lot #____________
Exp. date _______
4._______ If applicable, verify that the calibrator has not reached the expiration date.
Lot #____________ Exp. date _______
5._______ After the maintenance has been completed, verify that the Background counts are within the
acceptable limits. Record the background counts below or attach a printout to this document.
WOC < 0.10 ________
NOC
< 0.10 ________
RBC
< 0.02 ________
HGB
< 0.10 ________
PLT
< 5.0
________
6._______ Verify the Open Mode precision by analyzing a fresh, normal whole blood sample 10 times in
succession. Select an empty QC file in the
SPECIMEN TYPE
menu. Make sure Open Mode is
selected. Run the sample 10 times. When the runs have been completed, write the CVs displayed
on the screen in the appropriate spaces below or attach a file printout to this document.
PARAMETER
CV% LIMIT
CV%
WOC
<
2.7%
________
NOC
<
2.7%
________
RBC
<
1.5%
________
HGB
<
1.0%
________
MCV
<
1.0%
________
PLT
<
4.0%
________
7.______
If the CV for all parameters fall within the limits, go to step 8 to verify Closed Mode precision. If a
parameter’s CV exceeds the limit, run the sample again. If the over-limit condition persists, call the
Abbott Hematology Customer Support Center for assistance.
