CELL-DYN
®
3200 System Operator’s Manual
5-83
9140181H—October 2001
Section 5
Operating Instructions
Sample Collection and Handling
Anticoagulant
All performance claims given in this manual were generated from samples
collected in K
3
EDTA.
Sample Stability
Any refrigerator-stored specimens should be brought to room temperature before
processing. If specimens are to be run within eight hours after collection, storage
at room temperature is recommended. If specimens are to be run more than eight
hours after collection, storage at temperatures between 2° and 8° C is
recommended.
Stability studies show that, when specimens are refrigerated at temperatures
between 2° and 8° C, and brought to room temperature before mixing and
processing, results for the RBC, HGB, MCV, PLT are stable (±5%) for up to 36
hours after collection. The total WBC and MPV is stable (±5%) for up to 24 hours
after collection. The stability of the total WBC decreases to ±10% at 36 hours after
collection. An increase in false-positive Suspect Population Flags may be seen on
samples processed less than 30 minutes or more than 4 hours after collection time.
The stability of capillary samples collected in microtainers may vary depending on
the microtainer manufacturer. Refer to the manufacturer’s package insert for
stability claims.
Sample Collection
All samples should be collected using proper technique and following the tube
manufacturer’s recommendations.
NOTE:
For additional information on collecting venous and capillary samples,
refer to NCCLS Standards, H3-A3
1
and H4-A3
2
.
WARNING: Potential Biohazard.
Consider all specimens, reagents,
controls, calibrators, etc., that contain human blood or serum as potentially
infectious. Wear gloves, lab coats, and safety glasses, and follow other
biosafety practices as specified in the OSHA Bloodborne Pathogen Rule
(29 CFR Part 1910.1030) or other equivalent biosafety procedures.
Samples that will be run on the Sample Loader must have a minimum volume of
1.5 mL to ensure proper mixing by the Sample Loader.
Samples that will be run on the Closed Sampler model must have a minimum
volume of 1.5 mL to ensure proper sampling.
A minimum of 180 µL should be collected for capillary samples. This ensures an
adequate amount of blood for the Open Mode (150 µL) aspiration.
