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CELL-DYN
3000 System Operator’s Manual
9140240E — May 1995
Principles of Operation Chapter 3
Sample Aspiration
There are two modes of whole blood sample aspiration on the CELL-
DYN 3000. The operator selects the mode of aspiration from the
•
The open sampler mode is used to aspirate the sample from a
collection tube that has been opened and is held under the open
sample aspiration probe.
•
The manual closed sampler or automated Sample Loader mode is
used to aspirate the blood directly from a collection tube by
piercing the tube stopper.
The aspiration volumes are:
•
Open Mode 170 µL ± 5%
•
Closed Mode (CS) 230 µL ± 5%
•
Sample Loader (SL) 350 µL ± 5%
Once the mode of aspiration is selected, the whole blood sample is
aspirated into the Analyzer by the sample aspiration peristaltic pump.
The pump aspirates the sample through the shear valve. An optical
sensor checks the integrity of the sample.
Sample Analysis Cycle Overview
NOTE: Sample and reagent volumes given in this section are
stated as the nominal values. Slight differences between
instruments may cause these volumes to vary. These differences
are compensated for by factory-set internal dilution factors.
Sample Segments
The shear valve rotates in order to isolate three segments of the whole
blood sample which has been aspirated through it. The segments are:
32 µL for the WBC dilution
0.64 µL for the RBC/PLT dilution
12 µL for the HGB dilution
WBC Analysis
1.
The WBC Sheath syringe dispenses 1.6 mL of WBC Sheath
reagent through the shear valve where it picks up the 32 µL WBC
segment.
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