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4titude Ltd. • The North Barn • Surrey Hills Business Park • Damphurst Lane • Wotton • Surrey • RH5 6QT

T: +44 (0)1306 884885 • F: +44 (0)1306 884886 • E: [email protected] • www.4ti.co.uk

4ti-FL-0010  Quick User Guide a4S Automatic Heat Sealer VA  en1/0/25/07.2015  OP

Automatic Heat Sealer a4S

Quick User Guide • 

Version A

Note: This quick user guide is not a replacement for the a4S Operation Manual which must be read before using the heat sealer 

for the first time. All users must know and observe all safety instructions as detailed in the operation manual. 

Important!

Shipping Bracket  

 

The a4S is shipped with a transportation bracket to prevent the plate shuttle moving during shipping. It is essential that this 

bracket is removed before the unit is powered up for the first time.

Sensor Protection Screw 

 

To protect the rear foil sensor during transportation, the a4S is shipped with a protective screw highlighted with a white washer, 

inserted into the rear of the instrument. Before the unit is switched on, please make sure that the screw is removed from the 

instrument and retained with the other transportation packaging. 
Please also refer to the 

a4S Operation Manual

.

Operation of the a4S Automatic Heat Sealer

1.  Connect the power cord and turn on the device. 
2.  Place the seal roll, along with its holder, on the device 

locating the ends of the Axle in either Seal Roll Support 1 

or 2. The orientation of the seal roll should be as indicated 

by the purple arrow. 

Seal Roll Support 1

(seal roll with large diameter)

Seal Roll Support 2

(seal roll with small diameter)

3.  Perform the seal roll loading procedure (Please refer 

to the a4S Operational Manual, section 2.4 

Seal Roll 

Loading Procedure

).

4. 

Perform the fine adjustment of the seal position (Please 

refer to the a4S Operational Manual, section 2.5 

Fine 

Adjustment of the Seal Position

).

5. 

Adjust sealing temperature and time settings (Please see 

reverse page).

6.  Place the plate to be sealed on the plate carrier, using 

an appropriate adaptor if necessary (Please see section 

Plate Adaptors

).

7.  The  

Seal

  icon will remain grey while the unit is heating 

or cooling. Once the set temperature is reached, the icon 

will turn green. 

8.  Sealing can then commence by touching  

Seal

Plate Adaptors

Plate Type / Plate Height

Adaptor / Side of adaptor to use

96well PCR plates

0116

14.4 mm

0120

26.54 mm

0136

41.20 mm

0960

16.1 mm

0384

10.6 mm

Adaptor B

Side with 96 holes

384well PCR plates

0116

14.4 mm

0120

26.54 mm

0136

41.20 mm

0960

16.1 mm

0384

10.6 mm

Adaptor B

Side with 384 holes

1536well plates,  

standard height plates

0116

14.4 mm

0120

26.54 mm

0136

41.20 mm

0960

16.1 mm

0384

10.6 mm

  4mm ‒ 20mm

Adaptor B

Side with 384 holes

Mid-height plates

0116

14.4 mm

0120

26.54 mm

0136

41.20 mm

0960

16.1 mm

0384

10.6 mm

14mm ‒ 33mm

Adaptor A

Deep well plates

0116

14.4 mm

0120

26.54 mm

0136

41.20 mm

0960

16.1 mm

0384

10.6 mm

34mm ‒ 53mm

No adaptor needed

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