3Brain BioCAM X, User Manual

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Sterilization procedure for BioChips
As indicated in the previous section, BioChips cannot be put in autoclaves, ovens or under UV-light
exposure for sterilization purpose. These procedures risk to deteriorate the chips resulting in not optimal
recording performances and in a shorter lifetime.
If still not done, perform a cleaning procedure as indicated in the “Cleaning procedure for BioChips”
previous section and adopt the following procedure (that has to be performed under a biological hood)
to sterilize the chips:
1. Clean the area of the BioChips outside the glass ring (including the external part of the glass ring
itself) by using a tissue soaked with ethanol 96% and put inside sterile plastic petri dishes.
2. Fill the reservoir completely with ethanol 70%. Also dip with the same solution the border of the glass
ring (the tiny upward-oriented side of the ring). Hence, wait for 20 minutes.
3. Suck the ethanol with a pipette or with a vacuum pump always using sterile tips.
4. Fill 3/4 of the reservoir of the BioChips with sterile DDW, wait few seconds and suck the liquid always
with sterile tips. Repeat these operations 4 times to ensure to wash out completely the ethanol. When
removing sterile DDW for the last time, be sure to have completely dried the chamber. Aspirate the
liquid on the recording area always by keeping the tip close to the glue/CMOS border but not touch -
ing the CMOS to avoid any risk to damage the electrodes.