10x Genomics 1000433, User Manual

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CG000542 | Rev A
User Guide | CytAssist Instrument | System Operation
Link to TOC | 10xgenomics.com
The Readiness Test calibrates the imaging parameters and verifies that
all systems are working optimally. No reagents are used (the Readiness
Test is the only dry run the instrument utilizes). Wear gloves when
performing the Readiness Test.
a.
During first-time setup, follow onscreen instruction to launch the
Readiness Test (~40 min).
To initiate a Readiness Test at any other time, navigate through the menu to System >
Alternate Modes > Readiness Test.
OR press the blue Run Start Button to initiate a run. Enter the Readiness Test serial
number (located on the Visium CytAssist Calibration Slide label).
b.
Locate the two blank slides and the Visium CytAssist Calibration
Slide in the Instrument Accessory Kit. Inspect for any chips or cracks.
Calibration slide may be cleaned with lens paper or laboratory wipes followed by
compressed air. Dirty slides may cause the readiness test to fail.
c. Enter the serial number on the calibration slide (using a different serial
number could result in poor calibration).
d. Open the instrument lid.
e. Follow onscreen instruction and load the blank slides and calibration
slide onto the instrument, as described in the Tips and Best Practices
section. DO NOT load reagents to the calibration slide for the Readiness
Test.
f.
Press the blue play button to start the test.
g.
A successful Readiness Test marks the end of setup.
Follow onscreen
instruction in the event of a failed or incomplete test.
Step 3:
Readiness Test
Spacer Spacer
Calibration Slide
Serial number for
readiness test on
Calibration Slide
label
Prior to running the
Readiness test, ensure
that the Shipping Bolt
has been removed.