GE AKTA GO User Manual Download

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ÄKTA

™

Operating Instructions

Original instructions

Summary of Contents for AKTA GO

Page 1: ...ÄKTA go Operating Instructions Original instructions ...

Page 2: ...als 42 4 2 Hardware installation 43 4 2 1 Connect the system 46 4 2 2 Install waste tubing 48 4 2 3 Prepare the pump rinsing system 51 4 2 4 Start the instrument 52 4 3 Software installation 53 4 3 1 Download and Install UNICORN 54 4 3 2 Download the Instrument Configuration 55 4 3 3 Adapt UNICORN to your system 57 4 4 Start UNICORN and connect to system 60 5 Prepare the system for a run 61 5 1 Pr...

Page 3: ... the main fuses 99 8 Troubleshooting 101 9 Reference information 102 9 1 System specifications 103 9 2 Chemical resistance specifications 106 9 3 Recycling information 108 9 4 Regulatory information 109 9 4 1 European Union and European Economic Area 110 9 4 2 Eurasian Customs Union 111 9 4 3 Regulatory statements 113 9 4 4 Declaration of Hazardous Substances DoHS 115 9 5 Health and Safety Declara...

Page 4: ... important information that must be read before operating the ÄKTA go system In this chapter See page Section 5 1 1 Important user information 6 1 2 About this manual 7 1 3 Associated documentation 4 ÄKTA go Operating Instructions 29360951 AC 1 Introduction ...

Page 5: ...tory staff members in research laboratories within academia and industry The ÄKTA go instrument must not be used in any clinical procedures or for diagnostic purposes The ÄKTA go is not intended for Reversed Phase Chromatography RPC since the EPDM membranes in the K9 Inlet valve are not compatible with buffers normally used in RPC Prerequisites In order to operate ÄKTA go in the way it is intended...

Page 6: ...Instructions cover the ÄKTA go system Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text for example Power Notes and tips A note is used to indicate information that is important for trouble free and optimal use of the ...

Page 7: ...ormation needed to install operate and maintain the ÄKTA go system in a safe way ÄKTA go Operating Instructions Translations of the original instructions are given in several languages Essential information to be kept near the ÄKTA go system ÄKTA go Cue Cards Additional detailed information on the system com ponent functions and maintenance Tips on how to get the most out of the system when runnin...

Page 8: ...p menu or press the F1 key on your keyboard The documents are available in the UNICORN Online Help and Documentation section Main contents Documentation Detailed instructions on how to install UNICORN UNICORN Quick Installation Guide 1 Overview and detailed description of network setup and complete software installation Administration of UNICORN and the UNICORN database UNICORN Administration and ...

Page 9: ...howing common workflows in the Evaluation module Overview of features of the Evaluation module Getting started with Evaluation accessed through help in the UNICORN Evaluation module Descriptions are displayed for the currently active pane or dialog box UNICORN Help 1 TheUNICORNQuickInstallationGuidecanbedownloadedfromwww gelifesciences com aktago 2 The current UNICORN version is also added to the ...

Page 10: ...er This chapter contains important information for your personal safety In this chapter See page Section 11 2 1 Safety precautions 14 2 2 Labels 15 2 3 Emergency procedures 10 ÄKTA go Operating Instructions 29360951 AC 2 Safety instructions ...

Page 11: ...NG WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood NOTIC...

Page 12: ...he product WARNING Do not use any accessories not supplied or recommended by GE Healthcare WARNING Do not use ÄKTA go if it is not working properly or if it has suffered any damage for example damage to the power cord or its plug damage caused by dropping the equipment damage caused by splashing liquid onto it WARNING Access to power plug Do not block access to the power outlet and power plug The ...

Page 13: ...s for safe operation and maintenance of this product WARNING Spread of biological agents The operator must take all necessary actions to avoid spreading hazardous biological agents The facility must comply with the national code of practice for biosafety WARNING High pressure The product operates under high pressure Wear protective glasses and other required Personal Protective Equip ment PPE at a...

Page 14: ...not open any covers or replace parts unless specifically stated in the user documentation Electrical rating Voltage VAC Voltage Electrical rating Frequency Hz Frequency Electrical rating Maximum power consumption VA Max Power Degree of protection provided by the enclosure Protection Class Year YYYY and month MM of manufacture Mfg Date 14 ÄKTA go Operating Instructions 29360951 AC 2 Safety instruct...

Page 15: ...ther the system is equipped with a Real Time Unit and whether the instrument or the computer is affected If power is lost to the ÄKTA go instrument with or without a Real Time Unit the run is interrupted immediately Run data collected up to the time of the power failure is saved in the UNICORN software If power is lost to the computer and the system is not equipped with a Real Time Unit the run is...

Page 16: ...t the instrument by pressing the On Off button on the instrument control panel 2 Start the computer and the UNICORN software 3 Re establish connection between UNICORN and the instrument 4 If the run has been aborted recover or discard remaining sample and flush the flow path as appropriate 5 16 ÄKTA go Operating Instructions 29360951 AC 2 Safety instructions 2 3 Emergency procedures ...

Page 17: ...er This chapter gives an overview of the ÄKTA go instrument and the UNICORN software In this chapter See page Section 18 3 1 ÄKTA go 24 3 2 Available modules 27 3 3 UNICORN ÄKTA go Operating Instructions 29360951 AC 17 3 System description ...

Page 18: ...m for accessories on the left hand side of the instrument Run Exterior design The liquid handling modules are placed on the front of the instrument The instrument is equipped with trays to collect spillage and adjustable feet to level the instrument Buffer vessels can be placed on top of the instrument Air ventilation power cables and data cables are located at the rear of the instrument 18 ÄKTA g...

Page 19: ... 11 Outlet valve 12 Inlet valve 13 Bottom tray 14 Ready 8 9 10 11 12 13 14 2 1 6 3 4 5 7 Illustration of the instrument control panel The instrument control panel is located to the upper left on the front of the instrument It shows the current status of the system using LED light and status text The pause and continue buttons can be used to control an ongoing run Control panel buttons can be locke...

Page 20: ...us indicators The display and status indicators on the instrument control panel indicate the current status of ÄKTA go The table below describes the different states that can be displayed Description Display State The instrument is turned off Off 20 ÄKTA go Operating Instructions 29360951 AC 3 System description 3 1 ÄKTA go ...

Page 21: ...h the UNICORN instrument server Offline The display toggles between Offline the instrument IP address and the Instrument serial number The instrument is connecting to the UNICORN instrument server Connecting The status indicator flashes a white light The instrument is ready to use Ready ÄKTA go Operating Instructions 29360951 AC 21 3 System description 3 1 ÄKTA go ...

Page 22: ...te Hold A system wash or a pump wash instruction is ongoing Wash The instrument has been paused due to an alarm or an instrument error After examining the cause of the error acknowledge the alarm and continue the run in UNICORN Alarms and errors The status indicator flashes a red light 22 ÄKTA go Operating Instructions 29360951 AC 3 System description 3 1 ÄKTA go ...

Page 23: ...g mode Power save The status indicator is half lit with a white light flashing slowly A module is being re programmed during an instrument configuration installation Re program ming ÄKTA go Operating Instructions 29360951 AC 23 3 System description 3 1 ÄKTA go ...

Page 24: ...es the pressure directly after the pump Pressure monitor R9 A 1 mL static mixer that mixes the buffers delivered by the pump Mixer An injection valve that injects sample onto the column Injection valve V9 J A LED UV monitor that measures the UV absorbance at 280 nm of buffers and eluted proteins Includes a UV cell with a path length of 2 mm An optional UV cell with a path length of 5 mm is availab...

Page 25: ... two directions upflow and downflow Column valve V9 Cm An advanced column valve that can connect up to five columns to the instrument Directs the flow onto one col umn at a time and allows for flow in two directions upflow and downflow This module contains two pressure sensors that enable pre column pressure and delta column pres sure signals Column valve V9 C A pH valve that enables in line monit...

Page 26: ... or otherwise locked due to for example software updates The method is automatically downloaded to the Real Time Unit when the method is started Instructions are sent from the Real Time Unit to the instrument and data are collected in the Real Time Unit during the run The result is uploaded from the Real Time Unit to the computer when the connection is re stored 26 ÄKTA go Operating Instructions 2...

Page 27: ... Method Editor Predefined methods with built in application support Drag and drop functionto buildmethodswithrelevant steps Method text editing Start monitor and control runs The current flow path is illustrated in the Process Picture which allows manual interactions with the system and provides feedback on run parameters System Control Open results and evaluate runs Evaluation The Evaluationmodul...

Page 28: ...module 1 3 4 5 2 Description Part The toolbar buttons are used for quick access to instrument controls 1 For descriptions see System Control toolbar buttons below The run data field shows the value of run data in boxes This field is hidden by default To make this field visible go to View run data 2 The Chromatogram pane illustrates the chromatogram of the run 3 The interactive Process Picture pane...

Page 29: ...button permanently ends the run Customize Documentation This button opens a dialog containing information about the system and the current run This button opens the Cus tomize dialog box where curve settings run data groups and run log con tents can be set Connect to Systems Column Handling This button opens the Col umn Handling tool which contains a column list with parameters for GE Healthcare c...

Page 30: ...e using the Process Picture Click on the different parts of the Process Picture pane to interact with the system For a complete list of manual instructions go to Manual Execute manual instructions 30 ÄKTA go Operating Instructions 29360951 AC 3 System description 3 3 UNICORN ...

Page 31: ...erform an installation of ÄKTA go In this chapter This chapter contains the following sections See page Section 32 4 1 Site preparation 42 4 2 Hardware installation 52 4 3 Software installation 57 4 4 Start UNICORN and connect to system ÄKTA go Operating Instructions 29360951 AC 31 4 Installation ...

Page 32: ... be met only if the laboratory environ ment fulfills the requirements stated in this chapter In this section See page Section 33 4 1 1 Delivery storage and unpacking 35 4 1 2 Space requirements 37 4 1 3 Site environment 39 4 1 4 Power requirements 40 4 1 5 Computer requirements 41 4 1 6 Required materials 32 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 1 Site preparation ...

Page 33: ... special equipment is needed When you receive the delivery Record on the receiving documents if there is any apparent damage on the delivery box Inform your GE Healthcare representative of such damage Move the delivery box to a protected location indoors Storage requirements The delivery box should be stored in a protected place indoors The following storage requirements must be fulfilled for the ...

Page 34: ...er at the front Do not tip the instrument when lifting The illustra tion below shows the recommended way to lift the ÄKTA go instrument 34 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 1 Site preparation 4 1 1 Delivery storage and unpacking ...

Page 35: ...ug The power cord with plug must always be easy to disconnect Laboratory bench The bench must be clean flat and stable and of sufficient size for the ÄKTA go system with accessories The computer is located on the right side of the instrument to make room for accessories on the left side of the instrument Run ÄKTA go Operating Instructions 29360951 AC 35 4 Installation 4 1 Site preparation 4 1 2 Sp...

Page 36: ...Parameter 335 mm W width 482 mm H height 464 mm D depth 27 kg Weight 10 kg Allowed weight of buffer bottles on the top tray 36 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 1 Site preparation 4 1 2 Space requirements ...

Page 37: ...perature operating 25 C to 60 C for 48 h Ambient temperature storage 20 to 95 non condensing Relative humidity Up to 2000 m1 Altitude operating Pollution degree 2 Pollution degree of the intended environment 1 If the installation is situated at a higher altitude the customer is responsible for establishing that the ÄKTA go instrument can be used safely in accordance with local regulations Instrume...

Page 38: ...eat output Component Typical 100 W ÄKTA go instrument Max 150 W Power save 20 W For heat output of the computer refer to the manufacturer s specifications 38 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 1 Site preparation 4 1 3 Site environment ...

Page 39: ... approved plugs delivered or approved by GE Healthcare The following table specifies the power requirements for the ÄKTA go instrument For power requirements for the computer refer to the manufacturer s specifications Requirement Parameter 100 to 240 V Supply voltage 50 60 Hz Frequency Overvoltage category II Transient overvoltages 300 VA Max power consumption ÄKTA go Operating Instructions 293609...

Page 40: ...o liquids and moisture General computer specifications For information on computer specifications see http www gelifesciences com UNICORNPCspecifications For information about compatibilitybetween UNICORN versions the supported operating systems database versions and instrument configuration see the UNICORN compati bility matrix at http www gelifesciences com UNICORNcompatibility Network connectio...

Page 41: ...ovided at the installation site Scope of use Required volume Buffer solution System test Pump test and Mixer test 1 L Distilled water If applicable Fraction Collector F9 R test and or Column Valve V9 C test System test and Mixer test 1 L 1 0 acetone and 1 0 M NaCl in distilled water Priming of the pump rinsing sys tem 150 mL 20 ethanol in distilled water ÄKTA go Operating Instructions 29360951 AC ...

Page 42: ... go In this section This section contains the following subsections See page Section 43 4 2 1 Connect the system 46 4 2 2 Install waste tubing 48 4 2 3 Prepare the pump rinsing system 51 4 2 4 Start the instrument 42 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 2 Hardware installation ...

Page 43: ... with plug must always be easy to disconnect WARNING Protective ground The product must always be connected to a grounded power outlet WARNING Power cord Only use power cords with approved plugs delivered or approved by GE Healthcare WARNING Supply voltage Before connecting the power cord make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument ÄKTA go Oper...

Page 44: ...orrect power cord to be used Each instrument is delivered with 2 alternative power cords 1 Power cord with US plug 2 m Power cord with EU plug 2 m Recycle the power cord that is not to be used Connect the power cord to the Power input connector on the back of the instrument and to a grounded power outlet 100 240 V 50 60 Hz 2 Connect the computer to a power source using the manufacturer s instruc t...

Page 45: ... the computer 2 Make sure that the IP address of the instrument is on the same subnet as the IP address of the port used in the computer Note The IP address of the instrument is displayed on the instrument control panel when the instrument is powered on but not connected to UNICORN 3 If the computer is to be connected to a network connect a network cable between the computer and a network wall out...

Page 46: ...he waste tubing CAUTION Fasten the waste tubing Make sure that tubing is securely fas tened to the waste ports W W1 and W2 CAUTION Make sure that the waste vessel will hold all the produced volume of the run For ÄKTA go a suitable waste vessel should typically have a volume of 2 to 10 liters CAUTION Cut injuries The tubing cutter is very sharp and must be handled with care to avoid injuries NOTICE...

Page 47: ...is important that the tubing is not bent 2 Note If the tubing is too short replace it with new tubing Do not lengthen the tubing as this might cause obstruction of the tubing Fasten all waste tubing to the waste vessel 3 ÄKTA go Operating Instructions 29360951 AC 47 4 Installation 4 2 Hardware installation 4 2 2 Install waste tubing ...

Page 48: ...e pump chamber and the drive mechanism of the pump Illustration of the pump rinsing system The illustration below shows the parts and tubing of the pump rinsing system 1 2 3 4 Description Part Inlet tubing 1 Outlet tubing 2 Rinsing solution tube holder 3 Rinsing solution tube 4 48 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 2 Hardware installation 4 2 3 Prepare the pump rinsing sys...

Page 49: ...w the rinsing solution tube back into the holder 3 Insert the inlet tubing into the solution in the rinsing solution tube Note Make sure that the inlet tubing reaches close to the bottom of the rinsing liquid tube 4 Connect a 25 to 30 mL syringe to the outlet tubing Draw liquid slowly into the syringe until the rinsing system tubing is filled 5 ÄKTA go Operating Instructions 29360951 AC 49 4 Insta...

Page 50: ...utlet tubing into the liquid in the rinsing solution tube 7 Unscrew the rinsing solution tube and fill it with 50 mL of 20 ethanol or aqueous buffer 8 50 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 2 Hardware installation 4 2 3 Prepare the pump rinsing system ...

Page 51: ...ument control panel displays a white light for approximately 2 seconds 2 The instrument is on but is not connected to the UNICORN instrument server 3 Result The displays toggles between Offline the instrument IP address and the instrument serial number ÄKTA go Operating Instructions 29360951 AC 51 4 Installation 4 2 Hardware installation 4 2 4 Start the instrument ...

Page 52: ...UNICORN system admin istrator at the site Detailed information about software installation and configuration is available in the UNICORN Administration and Technical Manual In this section This section contains the following subsections See page Section 53 4 3 1 Download and Install UNICORN 54 4 3 2 Download the Instrument Configuration 55 4 3 3 Adapt UNICORN to your system 52 ÄKTA go Operating In...

Page 53: ...the welcome dialog box click Next 3 Select installation type Full installation or Custom installation and click Next Note Any missing prerequisites will be installed The computer might need to be restarted several times during the installation 4 Download a e license from the e Delivery portal and configure the e license for the UNICORN installation 5 Adapt UNICORN to your system see Section 4 3 3 ...

Page 54: ...lifesciences com aktago 1 Click RELATED DOCUMENTS 2 Click SOFTWARE 3 Download the Instrument configuration software 4 Unzip the downloaded file to a folder on the local computer 5 Use the downloaded instrument configuration to define your system see Section 4 3 3 Adapt UNICORN to your system on page 55 6 54 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 3 Software installation 4 3 2 D...

Page 55: ... System Properties Define System 2 Choose the instrument configuration that was downloaded System Type and Setup Option Click Next 3 Choose a system name and enter the serial number of the instrument Note The serial number is found on the back of the instrument and on the instrument control panel when the instrument is offline 4 Click Finish 5 In the Administration module click System Properties E...

Page 56: ...apt UNICORN to your system including system setup is found in the UNICORN Administration and Technical Manual Note 56 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 3 Software installation 4 3 3 Adapt UNICORN to your system ...

Page 57: ... ment the e license needs to be downloaded and configured for the computer the system has to be defined with the correct modules in UNICORN Start UNICORN and log on Follow the instructions to start UNICORN and log on to the program Action Step Double click the UNICORN icon on the desktop 1 In the Log On dialog box select User Name and enter Password 2 Tick the boxes for the UNICORN modules that yo...

Page 58: ...tem Control module click the Connect to Systems button 1 In the Connect to Systems dialog box 2 Select a system checkbox Click Control for that system Click OK Tip If UNICORN is unable to connect to the selected instrument try restarting the computer See Chapter 8 Troubleshooting on page 99 for more information 58 ÄKTA go Operating Instructions 29360951 AC 4 Installation 4 4 Start UNICORN and conn...

Page 59: ... UNICORN Follow the steps below to activate the Power save function Action Step In System Control click System Settings Advanced 1 Turn on the Power save function 2 Enter a Time for the Power save 3 Click OK 4 ÄKTA go Operating Instructions 29360951 AC 59 4 Installation 4 4 Start UNICORN and connect to system ...

Page 60: ...tilation meets the local requirements CAUTION Fire hazard Before the system is turned on make sure that there is no unintentional leakage of flammable liquids in ÄKTA go In this chapter This chapter contains the following sections See page Section 61 5 1 Prepare the flow paths 64 5 2 Prime inlets and purge pump heads 70 5 3 Performance tests 74 5 4 Connect a column 77 5 5 Pressure alarms 78 5 6 Pr...

Page 61: ...e used Shattered glass from falling bottles might cause injury Spilled liquid may cause fire hazard and personal injury CAUTION Max weight on top tray Do not place containers with a volume of more than 2 liters each on the top tray The total allowed weight on the top tray is 10 kg CAUTION Avoid spillage and overflow during collection Make sure that outlet tubing is connected to the outlet Out1 or ...

Page 62: ...ration is defined by the user in the UNICORN Administration module A B C Sample 2 1 3 4 5 6 7 8 9 10 11 Description Part Description Part UV monitor U9 L 7 Inlet valve K9 1 Conductivity monitor C9 8 Pump P9 S 2 Flow restrictor FR 902 9 Pressure monitor R9 3 Outlet valve V9 Os 10 Mixer 4 Fraction collector F9 R 11 Injection valve V9 J 5 Column 6 62 ÄKTA go Operating Instructions 29360951 AC 5 Prepa...

Page 63: ...n collector is used make sure that 400 mm tubing is connected between the fraction collector and the Frac port on the outlet valve and prepare the fraction collector for a run For more information see the Fraction collector F9 C and F9 R Operating in structions Plug unused valve ports It is recommended to plug all unused valve ports with stop plugs before starting a run See ÄKTA go User Manual for...

Page 64: ...rm that it is free from air Prime the inlet tubing Follow the steps below to prime all inlet tubing that is to be used during the run Action Step Make sure that all inlet tubing that is to be used during the run is placed in the correct buffer 1 Open the System Control module 2 In the Process Picture pane click on Inlet Valve and select one of the inlets to be primed 3 Result The inlet valve opens...

Page 65: ...owly into the syringe until liquid reaches the pump and the inlet tubing is filled with liquid 5 Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents 6 Repeat steps 3 to 6 for each inlet that is to be used during the run 7 ÄKTA go Operating Instructions 29360951 AC 65 5 Prepare the system for a run 5 2 Prime inlets and purge pump heads ...

Page 66: ...k Injection valve and select Waste 1 Result The injection valve switches to waste position and opens inlet A In the Process Picture pane click Inlet Valve and select the inlet that will be used at the beginning of the run 2 Result The inlet valve opens the selected inlet 66 ÄKTA go Operating Instructions 29360951 AC 5 Prepare the system for a run 5 2 Prime inlets and purge pump heads ...

Page 67: ...clockwise about one and a half turns Draw liquid slowly into the syringe at a rate of approximately 1 mL per second until there are no air bubbles in the liquid that reaches the sy ringe 5 Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents 6 ÄKTA go Operating Instructions 29360951 AC 67 5 Prepare the system for a run 5 2 Prime inlets and purge pump heads ...

Page 68: ...essure curve If the pressure does not stabilize within a few minutes there could be air left in the pump Note The pressure signal is considered stable if the fluctuation is no more than 5 up or down See examples below 8 Stable pressure no air in the Pump 68 ÄKTA go Operating Instructions 29360951 AC 5 Prepare the system for a run 5 2 Prime inlets and purge pump heads ...

Page 69: ... few minutes repeat the procedures to prime the inlet tubing and purge the pump If the pressure still does not stabilize see the ÄKTA go User Manual for further instructions 9 ÄKTA go Operating Instructions 29360951 AC 69 5 Prepare the system for a run 5 2 Prime inlets and purge pump heads ...

Page 70: ...ould be performed The Pump test is a shorter test that can be run to test the pump This is recommended if the pump has been replaced or undergone maintenance Details of the individual tests including purpose and required material are given in the method notes for each test method The performance tests are always run without a column Note Procedure Performance tests are provided with the instrument...

Page 71: ...in the ÄKTA go system Attempting to run a test for a module that is not in stalled will generate an error message Note The dialog for selecting and running a performance test includes a tab with the heading System Performance Report This tab does not contain the re ports for the performance tests Read the information in the Method Notes panel carefully Click Run Perfor mance Test 3 ÄKTA go Operati...

Page 72: ...t Click Next to continue Note A printer must be installed on the UNICORN Instrument Server to be able to print the report see the UNICORN Administration and Technical Manual for details 4 72 ÄKTA go Operating Instructions 29360951 AC 5 Prepare the system for a run 5 3 Performance tests ...

Page 73: ... test was passed or failed in the System Performance Report which is located at C Program Files x86 GE Healthcare UNI CORN UNICORN x x Temp where x x is the UNICORN version number The test result is stated at the top of the report If the test was failed refer to ÄKTA go User Manual for possible causes 7 The progress of the performance test is shown in the Chromatogram pane in the System Control mo...

Page 74: ...pressure of the column Before connecting a column to the ÄKTA go instrument read the instructions for use of the column CAUTION Cut injuries The tubing cutter is very sharp and must be handled with care to avoid injuries Methods automatically include a pressure alarm based on the specifications of the chosen column When performing a manual run you must set the pressure limits yourself See Section ...

Page 75: ...lve Note Use red fingertights for columns with a red top and black for other columns 3 In the Process Picture pane select Pump enter a low System flow e g 0 2 mL min and click Set 4 Result A system flow of 0 2 mL min starts Note If your system is equipped with a column valve make sure to start a flow in the correct position in the column valve and connect the column to those positions In the Proce...

Page 76: ...mns Overtightening might rupture the connectors or squeeze the tubing and thereby result in high back pressure Cut a piece of tubing with appropriate length to connect the bottom of the column to the UV monitor Remove the stop plug from the bottom of the column and connect this tubing in its place 8 When buffer is dripping from the tubing connected to the bottom of the column connect this piece of...

Page 77: ...he specifications of the chosen column When performing a manual run you must set the pressure limits yourself There is a default pressure limit of 2 MPa but this limit does not protect all columns Note Set a pressure alarm in a method In a method the pressure alarm is set in Method Settings See Section 6 1 Create a method on page 80 Set a pressure alarm in a manual run In a manual run the pressure...

Page 78: ...avoid overheating NOTICE Place the computer in room temperature If the ÄKTA go instru ment is placed in a cold room use a cold room compatible comput er or place the computer outside the cold room and use the Ether net cable delivered with the instrument to connect to the computer When the instrument is kept in a cold room it is important to retighten all tubing connectors and inlet connectors bec...

Page 79: ...our method In this chapter See page Section 80 6 1 Create a method 82 6 2 Prepare sample for loading 84 6 3 Start a method run 85 6 4 Monitor or interact with the run 86 6 5 Evaluate the run 87 6 6 Procedures after the run ÄKTA go Operating Instructions 29360951 AC 79 6 Run a method ...

Page 80: ...nformation Action Step Open the Method Editor module click the New Method button and choose the system and a suitable predefined method 1 Open the Phase Properties tab and confirm that all of the selections made in the phases correspond to your intended method by following the steps below 2 In the Method Settings phase select column type to get suitable values or enter correct values at Column vol...

Page 81: ...lume and concentration of buffer B and whether a fraction collection is to be performed 6 In the Elution phase set how to elute the sample the duration of the elution the concentration of buffer B and how the eluted sample will be collected 7 In the Re equilibration phase choose a suitable volume and concentration of buffer B 8 Click the save button choose the system name and location for your met...

Page 82: ...ion valve ports LoopF fill and LoopE empty 1 Fill a syringe with sample 2 Connect the syringe to the injection valve port Syr 3 In the Process Picture make sure that the injection valve is in position Load Note Load is the default position for the valve 4 Load sample into the sample loop To avoid sample loss due to siphoning leave the syringe in the port until the sample has been injected onto the...

Page 83: ...d select Sample Result The inlet valve opens the Sample inlet 2 Connect a 25 to 30 mL syringe to the purge valve of one of the pump heads 3 Open the purge valve by turning it counter clockwise one and a half turns Draw liquid slowly into the 25 to 30 mL syringe until the liquid reaches the inlet valve 4 Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents 5...

Page 84: ...pty volume No tubing is twisted and the flow path is free from leakage If a Fraction collector is being used that the correct tubes are used and loaded Start a method run Follow the steps below to start the method run Action Step Open the System Control module and click the button Open Method Navi gator 1 Select the method to run and click the Run button 2 Step through the displayed pages in the S...

Page 85: ... the Pause and Continue button on the instrument control panel When the method is completed the run stops automatically The pump stops the valves return to their default positions and the result is saved Which panes are visible in the System Control module can be customized in View To further customize these panes use the following steps Action Step Open the System Control module and click the Cus...

Page 86: ...and click Open 2 To save an evaluation file click File Save 3 Illustration of an evaluated run The image below shows an example of the features available in the Evaluation module in UNICORN 7 4 1 2 Function Part To integrate or remove integration click the fx button next to the curve name To adjust integration parameters go to the Peak tab 1 Data from the integration is shown in a Peak table below...

Page 87: ...lect the column being used 3 In Column CIP phase click get suggested steps Note The suggested steps are only available for some techniques and might not be optimized for your column Read the instructions provided with the column to get the recommended CIP steps 4 Click the save button choose the system name and location for your method and click Save 5 Run a column CIP To clean the column using th...

Page 88: ...plug the top and bottom of the column and place in a refrigerator 6 System storage If the system is not going to be used for a couple of days or longer clean the system using the System CIP method see Section 7 2 Perform system Cleaning In Place CIP on page 92 and then put the system in storage solution Start by removing the cleaned column from the system see above To put the system in storage fol...

Page 89: ...ress the On Off button on the instrument control panel The circular illumi nation flashes with white color while the instrument is shutting down 2 WARNING Power is still supplied to some internal electronics circuits when the instrument is switched off using the On Off button Disconnect the instrument from the power supply before maintenance or ser vice ÄKTA go Operating Instructions 29360951 AC 8...

Page 90: ... see the ÄKTA go User Manual WARNING All maintenance procedures inside the instrument chassis must be performed by a GE Healthcare service representative In this chapter See page Section 91 7 1 Clean the instrument externally 92 7 2 Perform system Cleaning In Place CIP 96 7 3 Replace pump rinsing liquid 97 7 4 Replace the main fuses 90 ÄKTA go Operating Instructions 29360951 AC 7 Maintenance ...

Page 91: ...ent or 20 ethanol Instruction Follow the steps below to clean the instrument externally Action Step Turn off the instrument and disconnect the power cord from the wall socket 1 Wipe the surface with a damp cloth Wipe off stains using a mild cleaning agent or 20 ethanol Wipe off any excess 2 Remove the top and bottom tray for cleaning Put them back once they are cleaned 3 Let the instrument dry com...

Page 92: ...path WARNING Hazardous biological agents during run When using hazardous biological agents run System CIP to flush the entire system tubing with bacteriostatic solution 1 M NaOH followed by a neutral buffer before service maintenance and decommissioning CAUTION Hazardous substances When using hazardous chemical and bio logical agents take all suitable protective measures such as wearing protective...

Page 93: ...h water 1M NaOH and buffer from inlets Sample C and A respectively The inlets used can be changed in each phase Follow the steps below to create a System CIP method Action Step In the Method Editor module click the Create New Method button 1 In the New Method dialog select System System CIP in the Predefined Method drop down list Click OK 2 Result A System CIP method is opened comprising the Metho...

Page 94: ...lutions Note The default solutions to use are buffer for inlet A 1M NaOH for inlet C and water for the Sample inlet 2 In the System Control module select the System CIP method created above and start the run 3 For complete cleaning of the flow path clean the manual injection port of the injection valve manually see the instructions below 4 Clean the syringe port of the injection valve Follow the s...

Page 95: ...they are secured in a waste container 3 Fill a syringe with approximately 10 mL of an appropriate cleaning solution 1 M NaOH or buffer solution Connect the syringe to injection valve port Syr and inject the cleaning solution 4 Fill a syringe with distilled water Connect the syringe to injection valve port Syr and inject the distilled water 5 ÄKTA go Operating Instructions 29360951 AC 95 7 Maintena...

Page 96: ...uid once a week if you are using 20 ethanol and daily if you are using an aqueous buffer For instructions see Section 4 2 3 Prepare the pump rinsing system on page 48 96 ÄKTA go Operating Instructions 29360951 AC 7 Maintenance 7 3 Replace pump rinsing liquid ...

Page 97: ...lacement do not continue to use the instrument Contact an authorized service engineer Required tools Dimension Tool 2 to 3 mm Flat screwdriver Procedure Follow the instructions below to replace the fuses There are two identical fuses at the mains power inlet Replace both fuses even if only one is blown Note Action Step Disconnect the power cord from the power inlet 1 ÄKTA go Operating Instructions...

Page 98: ... 3 Remove the fuses from the fuse holder 4 Fit new fuses of the same size and rating1 5 Replace the fuse holder in the power inlet 6 NOTICE When replacing fuses make sure that the fuse holder is pushed fully into position Reconnect the power cord 7 1 Fuse rating T4AL 250 V 5 20 mm 98 ÄKTA go Operating Instructions 29360951 AC 7 Maintenance 7 4 Replace the main fuses ...

Page 99: ... page 64 There is air in the system Prime the inlets and purge the pump see Section 5 2 Prime inlets and purge pump heads on page 64 There is air in the pump Unstable conductivity signal Prime the inlets and purge the pump see Section 5 2 Prime inlets and purge pump heads on page 64 There is air in the pump Unstable pressure Lower the flow rate The pressure is too high possibly due to running the ...

Page 100: ...r report When you request troubleshooting assistance from GE Healthcare you should generate a System error report and submit it to your service representative Follow the instructions below to generate a System error report Action Step Select System Create System Error Report in the System Control module 1 Users with sufficient access rights can also create a System error report from the Administra...

Page 101: ...ion In this chapter See page Section 102 9 1 System specifications 103 9 2 Chemical resistance specifications 106 9 3 Recycling information 108 9 4 Regulatory information 115 9 5 Health and Safety Declaration Forms ÄKTA go Operating Instructions 29360951 AC 101 9 Reference information ...

Page 102: ...uter columns buffer bottles 100 to 240 V autorange Supply voltage 10 from the nominal voltage Maximum voltage fluctuation 50 60 Hz Frequency Rated max 300 VA1 Power consumption Max with all options 150 W2 Typical 100 W Power save 20 W IP 21 Enclosure protective class 60 dB A Acoustic noise level 1 ÄKTA go can deliver 300 VA 2 ÄKTA go equipped with all options consume 150 W Battery information The ...

Page 103: ...pplies to the ÄKTA go flow path Recommended solutions for the pump rinsing system are aqueous buffers or 20 ethanol Do not use other chemicals in the pump rinsing system Chemical resistance long term The following chemicals are suitable for continuous use EC no CAS no Concentration Chemical N A N A N A Aqueous buffers pH 2 to 12 200 662 2 67 64 1 10 Acetone 231 635 3 7664 41 7 30 Ammonia 235 186 4...

Page 104: ... 500 018 3 9005 64 5 1 Tween 20 200 315 5 57 13 6 8 M Urea Chemical resistance short term The following chemicals are suitable for up to 2 h contact time at room temperature EC no CAS no Concentration Chemical 200 835 2 75 05 8 70 Acetic acid N A N A 10 Decon 90 200 837 3 75 08 1 100 Ethanol 231 595 7 7647 01 0 0 1 M Hydrochloric acid1 200 661 7 67 63 0 100 Isopropanol 200 659 6 67 56 1 100 Methan...

Page 105: ... in the pressure monitor or in the column valve V9 C For other parts of the system up to 1 M HCl is acceptable for short periods of use For cleaning of columns with HCl concentrations exceeding 0 1 M manually fill a loop with HCl and inject the cleaning agent ÄKTA go Operating Instructions 29360951 AC 105 9 Reference information 9 2 Chemical resistance specifications ...

Page 106: ...sonal protective equipment when de commissioning the equipment Decontamination The product must be decontaminated before decommissioning All local regulations must be followed with regard to scrapping of the equipment Disposal of the product When taking the product out of service the different materials must be separated and recycled according to national and local environmental regulations Recycl...

Page 107: ...erning the decommissioning of the equipment Disposal of batteries Waste batteries must not be disposed of as unsorted municipal waste and must be collected separately Follow applicable local regulations for recycling of batteries If equipped with a Real Time Unit the instrument contains a lithium battery which must not be disposed of in fire ÄKTA go Operating Instructions 29360951 AC 107 9 Referen...

Page 108: ...thcare Bio Sciences AB Name and address of manufacturer Björkgatan 30 SE 751 84 Uppsala Sweden 46 771 400 600 Telephone number of manufacturer In this section See page Section 109 9 4 1 European Union and European Economic Area 110 9 4 2 Eurasian Customs Union 111 9 4 3 Regulatory statements 113 9 4 4 Declaration of Hazardous Substances DoHS 108 ÄKTA go Operating Instructions 29360951 AC 9 Referen...

Page 109: ...ded with the product a copy of the EU Declaration of Conformity is available on request CE marking The CE marking and the corresponding EU Declaration of Conformity is valid for the in strument when it is used according to the Operating Instructions or user manuals and used in the same state as it was delivered except for alterations described in the Operating Instructions or user manuals ÄKTA go ...

Page 110: ... Importer and or company for obtain ing information about importer GE Healthcare Life Sciences Presnenskaya nab 10C 12th floor RU 123 317 Moscow Russian Federation Telephone 1 7 495 411 9714 Fax nr 7 495 739 6932 Email LSrus ge com Description of symbol on the system label The following symbol may be present on the system label Meaning Label Eurasian Conformity mark the single conformity mark indi...

Page 111: ...care could void the user s authority to operate the equipment Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide rea sonable protection against harmful interference when the equipment is operated in a commercial environment This equipmentgenerates uses and can radiateradio fr...

Page 112: ...use in a business environment When used in a residential environment there is a concern of radio interference 주의사항 A급 기기 업무용 방송통신 기자재 이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받은 기기 로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습니 다 112 ÄKTA go Operating Instructions 29360951 AC 9 Reference information 9 4 Regulatory information 9 4 3 Regulatory statements ...

Page 113: ...Use Period EFUP which indicates the period during which the hazardous substances contained in electrical and elec tronic products will not leak or mutate under normal operating conditions so that the use of such electrical and electronic products will not result in any severe environmen tal pollution any bodily injury or damage to any assets The unit of the period is Year In order to maintain the ...

Page 114: ...B T 26572规定的限量要求此表所列数据为发布时所能获得的最佳信息 0 Indicates that this hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in GB T 26572 X Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB T 26572 Data listed in the table represents best information a...

Page 115: ...pe testorothersuitablesurvey Adequate space andclearance is providedto allowsafe access forinstrumentservice repairor installation Insome cases this mayrequirecustomerto move equipmentfrom normaloperatinglocation prior to GE arrival Consumables suchas columns orgels have beenremovedor isolatedfromthe instrument andfrom any areathat may impede access tothe instrument All buffer waste vessels are la...

Page 116: ...ious orhazardous biological substances pleasespecify OtherHazardous Chemicals please specify Equipment must be decontaminatedpriortoservice return Pleaseprovide atelephone number whereGE cancontact youfor additionalinformationconcerningthe system equipment Telephone No Liquid and orgas in equipment is Water Ethanol None empty Argon Helium Nitrogen Liquid Nitrogen Other please specify Equipment typ...

Page 117: ...h supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 100 Results Way Marlborough MA 01752 USA GE Healthcare ...

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